Shanghai Shiping SPH-304 Orbital Decolorization Shaker

Overview

The Shanghai Shiping SPH-304 Orbital Decolorization Shaker is a precision-engineered laboratory shaker designed specifically for consistent, low-shear mixing in molecular biology, biochemistry, and histology workflows. Unlike general-purpose orbital shakers, the SPH-304 is optimized for decolorization, staining, blotting, and hybridization procedures—applications requiring uniform motion at controlled speeds without thermal input. Its orbital motion (20 mm diameter) generates gentle yet effective fluid agitation that minimizes sample foaming and pellet disruption while ensuring thorough reagent contact across membranes, gels, or tissue sections. The device operates exclusively at ambient temperature, eliminating thermal drift during time-sensitive incubation-free protocols such as Coomassie or silver stain removal, antibody stripping, or pre-hybridization washes. Built around a single-axis balanced drive system and governed by fuzzy logic control, the SPH-304 delivers high reproducibility in speed regulation (±1 rpm accuracy) and stable platform dynamics over extended operation cycles.

Key Features

• Fuzzy logic-based digital control ensures precise speed maintenance across the full 30–300 rpm range, with real-time feedback adjustment to compensate for load variance.
• LED display provides intuitive, glare-resistant readout of set speed, elapsed time, and remaining timer duration.
• Single 320 × 300 mm platform constructed from corrosion-resistant stainless steel, compatible with standard labware including gel trays, blotting cassettes, multi-well plates, and conical flasks (up to 250 mL × 9 units).
• Natural convection design eliminates internal heating elements, preserving ambient thermal integrity—critical for protocols sensitive to temperature-induced protein denaturation or nucleic acid degradation.
• ABS engineering plastic housing offers chemical resistance, structural rigidity, and ESD-safe operation in shared core facilities.
• Compact footprint (300 × 390 × 160 mm) and low power draw (60 W) support benchtop deployment in biosafety cabinets, cold rooms, or high-density lab environments.

Sample Compatibility & Compliance

The SPH-304 accommodates a broad spectrum of life science consumables: polyacrylamide and agarose gels (up to 20 × 20 cm), nitrocellulose/PVDF membranes, glass slides, microcentrifuge tubes (in rack adapters), and multi-well plates (6–96-well formats). Its 4 kg maximum platform load supports stacked tray configurations for high-throughput processing. While the unit does not incorporate active temperature regulation, its ambient-only operation aligns with ISO 15195:2018 guidelines for non-thermal instrumentation used in diagnostic assay development. The device meets IEC 61010-1 safety standards for laboratory electrical equipment and complies with RoHS Directive 2011/65/EU for hazardous substance restriction. No CE marking is applied due to domestic manufacturing scope; however, technical documentation supports third-party conformity assessment for export integration.

Software & Data Management

The SPH-304 operates via embedded firmware without external software dependency. All operational parameters—including speed setpoint, timer duration, and runtime status—are retained in non-volatile memory and persist through power cycling. The absence of network connectivity or data logging interfaces reflects its design intent: a dedicated, interference-free instrument for routine manual protocols. For laboratories operating under GLP or GMP frameworks, the deterministic behavior of fuzzy logic control and mechanical timer repeatability support audit-ready process documentation. Optional calibration certificates (traceable to NIM, China) may be provided upon request to verify rotational accuracy and timing fidelity against ISO/IEC 17025-accredited references.

Applications

• Decolorization of SDS-PAGE gels post-staining using methanol/acetic acid solutions.
• Wash steps in Western blotting (e.g., TBST agitation for antibody removal or blocking buffer equilibration).
• Pre-hybridization and post-hybridization agitation in Southern/Northern blot workflows.
• Cell suspension homogenization prior to centrifugation in primary culture labs.
• Agitation of ELISA plates during coating, blocking, or substrate incubation phases where temperature stability is essential.
• Consistent mixing of chromatin immunoprecipitation (ChIP) wash buffers without vortex-induced shearing.

FAQ

Does the SPH-304 support programmable multi-step protocols?
No. It features a single-stage timer (0–120 min) and fixed-speed operation per run; sequential speed or time changes require manual intervention.
Can the platform accommodate custom fixtures or adapter plates?
Yes. The flat, threaded platform surface allows secure mounting of user-fabricated or third-party accessories, provided total mass remains ≤4 kg and center-of-gravity stays within the 320 × 300 mm envelope.
Is the unit suitable for use inside a CO₂ incubator or cold room?
It is rated for ambient operation only (10–35°C, <80% RH non-condensing); internal condensation or elevated CO₂ levels may compromise electronic longevity and are not validated.
What maintenance is required for long-term reliability?
Annual inspection of drive belt tension (if applicable), cleaning of ventilation grilles, and verification of platform leveling using a spirit level—no lubrication or recalibration is needed under normal usage.
Are replacement parts available internationally?
Shanghai Shiping supplies OEM components—including control boards, LED displays, and motor assemblies—via authorized distributors; lead times average 4–6 weeks for overseas shipment.