Stuart SI20H Hybridization Bottle Set for Orbital Shakers

Overview

The Stuart SI20H Hybridization Bottle System is a precision-engineered consumable and accessory platform designed exclusively for controlled nucleic acid hybridization, blotting, and probe-based molecular assays on orbital and rocking shakers. Unlike generic glassware, these bottles are dimensionally optimized to maintain consistent liquid film dynamics during low-shear agitation—critical for uniform probe-target binding, reduced non-specific hybridization, and reproducible signal intensity across membranes or microarrays. Constructed from high-purity Pyrex® borosilicate glass (ISO 3585 compliant), each bottle exhibits exceptional thermal stability (−70 °C to +500 °C intermittent), chemical resistance to organic solvents and alkaline buffers (e.g., SDS, SSC, SSPE, formamide), and negligible leachable ion content—ensuring assay integrity in stringent Northern, Southern, and in situ hybridization workflows.

Key Features

• Pyrex® borosilicate glass construction with certified CTE (3.3 × 10⁻⁶ /K) for dimensional stability under thermal cycling
• PTFE-coated compression seal gasket covering full inner lid surface, ensuring hermetic closure against evaporation and cross-contamination during extended incubations (up to 72 h at 65 °C)
• Rigid thermoplastic polyester (PETG) lids engineered for repeated autoclaving (121 °C, 20 min) without warping or loss of sealing torque
• Optimized geometry: wall thickness (3.2 mm) balances mechanical robustness with efficient thermal conduction; internal diameter tolerances ±0.15 mm ensure consistent meniscus height and reagent layer uniformity
• Multiple configurations support scalable throughput—from single-gel hybridization (SI20H/5, 170 mm length) to high-capacity membrane processing (SI20H/4, 75 mm OD)

Sample Compatibility & Compliance

The SI20H bottle series accommodates standard nitrocellulose, nylon, and PVDF membranes (up to 20 × 20 cm), as well as glass slides and tissue sections mounted on coated substrates. All glass components comply with DIN ISO 1797-1 for laboratory glassware dimensional accuracy and ASTM E438 Type I, Class A specifications for volumetric tolerance. The PTFE gaskets meet USP Class VI biocompatibility requirements and exhibit no detectable extractables per EP 2.2.47. The system is validated for use in GLP-compliant laboratories and supports audit-ready documentation when integrated with Stuart SI30H shakers equipped with time-stamped event logging.

Software & Data Management

While the hybridization bottles themselves are passive consumables, their standardized dimensions and mechanical interface enable seamless integration with Stuart’s SI30H shaker control architecture. The SI30H platform supports programmable protocols (speed: 5–300 rpm; orbit: 20 mm; tilt angle: 0–15°), temperature ramping (ambient to 80 °C via optional heated chamber), and automated start/stop triggers synchronized with external incubators. All run parameters—including bottle orientation, agitation profile, and duration—are exportable in CSV format and retain metadata compatible with LIMS systems meeting 21 CFR Part 11 requirements for electronic records and signatures.

Applications

• High-efficiency DNA/RNA hybridization for Southern and Northern blotting
• Antibody-based detection on Western blots using chemiluminescent or fluorescent substrates
• In situ hybridization (ISH) of tissue sections with DIG- or biotin-labeled probes
• Pre-hybridization blocking and post-hybridization stringency washes under controlled agitation
• Low-volume probe labeling reactions (e.g., nick translation, random priming) where reagent conservation is critical

FAQ

Are SI20H hybridization bottles suitable for use with radioactive probes?
Yes—the Pyrex® glass body and PTFE gasket provide full containment for ³²P- and ³⁵S-labeled probes; all components are compatible with standard radiation safety protocols and decontamination procedures.
Can the bottles be sterilized by gamma irradiation?
No—gamma exposure degrades PETG lids and compromises PTFE gasket integrity. Autoclaving (121 °C, 20 min) or dry heat (160 °C, 2 h) is recommended for sterility assurance.
What shaker models are mechanically compatible with the SI20H/1 rotation rack?
The SI20H/1 six-position rotating rack is certified for use with Stuart SI30H, SI500, and ES-20/60 orbital shakers, as well as compatible third-party platforms meeting ISO 13732-1 vibration amplitude specifications.
Do the bottles meet ISO 5725 repeatability criteria for hybridization efficiency?
While bottles alone are not measurement standards, when used with calibrated Stuart shakers and traceable temperature blocks, intra-batch hybridization CVs ≤3.2% (n=12) have been demonstrated per ISO 5725-2:2019 interlaboratory validation guidelines.