Biohit mLINE 725080 Single-Channel Manual Adjustable Pipette
| Brand | Biohit |
|---|---|
| Origin | Finland |
| Model | 725080 |
| Type | Single-channel manual adjustable pipette |
| Channel count | 1 |
| Volume range | 1–10 mL |
| Sterilization | Full autoclaving (121 °C, 20 min) |
| Operating principle | Air displacement |
| Spring mechanism | Patented tension-spring piston system |
| Weight | Lightest in class (≤95 g for 10 mL variant) |
| Tip ejection | Independent mechanical push-off |
| Filtration | Integrated replaceable conical filter (≥10 µL compatible) |
| Grip design | Ergonomic, ambidextrous, with hanging hook |
| Volume lock | Dedicated volume locking collar |
| Material | Thermally insulating polymer housing |
Overview
The Biohit mLINE 725080 is a single-channel, manually operated, air-displacement pipette engineered for precision liquid handling across research, clinical, and quality control laboratories. Designed and manufactured in Finland, it operates on a patented tension-spring piston mechanism—distinct from conventional compression-spring systems—delivering uniform actuation force across its full 1–10 mL volume range. This architecture reduces required plunger pressure by up to 75% compared to standard pipettes, significantly lowering operator fatigue during high-throughput workflows. Its ultra-lightweight construction (≤95 g), thermally insulating housing, and ergonomic grip minimize thermal transfer to the internal piston assembly, preserving volumetric accuracy under variable ambient conditions. The device complies with ISO 8655-2:2022 for gravimetric performance testing and meets functional requirements outlined in ISO/IEC 17025 for accredited calibration laboratories.
Key Features
- Patented tension-spring piston technology ensures consistent, low-force aspiration and dispensing—regardless of set volume—reducing musculoskeletal strain during repetitive use.
- Full autoclavability at 121 °C for 20 minutes without disassembly; validated per ISO 17665-1 for steam sterilization integrity.
- Integrated conical filter (≥10 µL compatibility) acts as a final barrier against aerosol and liquid ingress into the piston chamber; filters are user-replaceable via dedicated plunger button.
- Ergonomic, ambidextrous handle with integrated hanging hook enables secure storage and minimizes bench clutter.
- Dedicated volume-lock collar prevents accidental volume drift during transport or multi-step protocols.
- Independent mechanical tip ejection mechanism decouples tip removal from plunger operation—eliminating unintended tip loss during dispense cycles.
- Thermally insulated polymer body mitigates heat transfer from hand to piston, maintaining metrological stability during extended handling.
Sample Compatibility & Compliance
The mLINE 725080 is compatible with universal low-retention and standard conical tips meeting ISO 8655-3 specifications. It supports aqueous solutions, buffers, diluted acids/bases, and common organic solvents (e.g., ethanol, isopropanol) within defined chemical resistance limits of its sealing materials (EPDM and PTFE). The device conforms to EN 61326-1 for electromagnetic compatibility and EN 61010-1 for laboratory equipment safety. While not intrinsically certified for hazardous environments, its non-sparking construction and static-dissipative housing meet general lab safety expectations. For GLP/GMP-regulated environments, traceable calibration certificates (per ISO/IEC 17025) and maintenance logs are supported through Biohit’s service network.
Software & Data Management
As a manually operated instrument, the mLINE 725080 does not incorporate onboard electronics or digital connectivity. However, it is fully compatible with external calibration management systems and electronic lab notebooks (ELNs) via documented calibration records and maintenance logs. Biohit provides downloadable calibration templates aligned with ISO 8655-5 and USP Analytical Instrument Qualification frameworks. All service documentation—including autoclave cycle validation reports and filter replacement logs—supports audit readiness for FDA 21 CFR Part 11-compliant workflows when maintained in controlled electronic systems.
Applications
This pipette is routinely deployed in applications requiring high repeatability and operator endurance, including cell culture media preparation, ELISA plate loading, PCR master mix assembly, QC sample dilution in pharmaceutical manufacturing, and environmental water analysis. Its stable thermal performance makes it suitable for cold-room or laminar flow hood use where hand temperature variability would otherwise compromise accuracy. The conical filter integration supports work with infectious agents (BSL-2), volatile compounds, and viscous samples prone to aerosol generation.
FAQ
Is the mLINE 725080 suitable for sterile applications?
Yes—full autoclavability at 121 °C for 20 minutes ensures sterility without compromising mechanical integrity or metrological performance.
How often should the conical filter be replaced?
Replace after each use involving potentially contaminated or volatile liquids; at minimum, inspect and replace every 100 pipetting cycles or upon visible saturation or deformation.
Can this pipette be calibrated in-house?
Yes—gravimetric calibration per ISO 8655-2 is feasible using Class A distilled water, certified analytical balances (≥0.0001 g resolution), and temperature-controlled environment (20 ± 0.5 °C). Biohit provides calibration SOPs and uncertainty budget templates.
Does the tension-spring mechanism affect long-term accuracy?
No—accelerated life testing (≥100,000 cycles) confirms no measurable hysteresis or drift in piston return force or volumetric delivery, validating sustained metrological reliability.
What tip types are recommended for optimal performance?
Use Biohit FlexiTip or other ISO 8655-3 compliant low-retention tips; avoid third-party tips with inconsistent cone geometry or excessive friction, which may compromise seal integrity or filter function.
