Agilent 1260 Infinity II Used High-Performance Liquid Chromatograph
| Brand | Agilent Technologies |
|---|---|
| Origin | USA |
| Model | LC-1260 |
| Maximum Pressure | 600 bar |
| Pump Delay Volume | as low as 120 µL |
| Flow Rate Range (Quaternary Pump) | 0.001–10 mL/min |
| DAD Data Acquisition Rate | up to 80 Hz |
| UV Noise Level | < ±0.6 mAU/cm (with 60 mm flow cell) |
| Typical Flow Precision | <0.15% RSD |
| Usage Age | 6–8 years |
| Warranty | 1 year post-installation |
Overview
The Agilent 1260 Infinity II HPLC system is a rigorously validated, pre-owned analytical platform engineered for high-resolution, high-throughput liquid chromatography in regulated and research environments. Built upon the proven architecture of the Agilent 1200 Series, this second-hand instrument delivers robust performance at 600 bar maximum system pressure—enabling method transfer from conventional HPLC to rapid resolution liquid chromatography (RRLC) without re-optimization. Its modular design supports full backward compatibility with established HPLC and RRLC methods, ensuring continuity in QC/QA workflows, stability-indicating assays, and impurity profiling across pharmaceutical, environmental, and food safety laboratories. The system operates on core principles of high-pressure gradient elution, precise solvent delivery via dual-piston positive displacement pumping, and real-time UV-Vis spectral acquisition using diode array detection (DAD), delivering reproducible retention time accuracy (<0.02 min RSD), peak area precision (<0.5% RSD), and baseline stability essential for trace-level quantitation.
Key Features
- 600 bar pressure capability supports sub-2 µm particle columns for enhanced resolution and reduced analysis time
- Low dwell volume pump configuration (≤120 µL) minimizes gradient delay and improves method scalability across column diameters (2.1 mm to 4.6 mm i.d.)
- Agilent’s proprietary capillary-flow cell DAD with 60 mm optical path achieves ultra-low noise (<±0.6 mAU/cm) and high sensitivity for trace analyte detection
- Infinity II autosampler employs a flow-through needle seat design—eliminating quantitative loop swaps while maintaining <0.25% RSD injection precision across volumes from 0.1 µL to 100 µL
- Integrated thermostat-controlled column compartment (5–80 °C) ensures retention time reproducibility and method robustness
- Full compliance-ready firmware supporting audit trail, electronic signatures, and user access control per FDA 21 CFR Part 11 requirements
Sample Compatibility & Compliance
The Agilent 1260 Infinity II accommodates a broad range of sample matrices—including aqueous extracts, organic solvent-solubilized APIs, plasma supernatants, and environmental water extracts—when paired with appropriate column chemistries (C18, phenyl-hexyl, HILIC, or ion-exchange). It meets critical regulatory benchmarks for analytical instrumentation: ISO/IEC 17025:2017 method validation readiness, USP chromatographic system suitability criteria, and ASTM E2913-21 guidelines for HPLC data integrity. All refurbished units undergo full functional verification per Agilent’s Factory Service Protocol—including pressure leak testing, gradient accuracy calibration, UV wavelength verification (using holmium oxide filter), and detector linearity assessment (0.001–2.0 AU)—prior to shipment. Documentation includes Certificate of Conformance, IQ/OQ summary report, and full service history log.
Software & Data Management
Controlled via OpenLab CDS ChemStation Edition or optional OpenLab ECM, the system provides secure, role-based workflow management with configurable electronic signatures, automated backup, and encrypted raw data storage (.D format). Audit trails record all method edits, sequence changes, and result modifications with timestamp, operator ID, and reason-for-change fields—fully traceable for GLP/GMP inspections. Data export supports CSV, PDF, and AIA/ANDI formats for LIMS integration. Optional 21 CFR Part 11 compliance package includes digital certificate management, biometric login support, and immutable archive generation compliant with ALCOA+ principles.
Applications
- Pharmaceutical QC: Assay, related substances, residual solvents, and dissolution testing per ICH Q2(R2) guidance
- Biopharmaceutical characterization: Monoclonal antibody purity assessment, glycan profiling, and charge variant analysis
- Environmental monitoring: Pesticide residue screening (EPA Method 531.1), PFAS quantification, and PAH analysis
- Food & beverage safety: Mycotoxin detection (aflatoxins, ochratoxin A), preservative quantitation, and vitamin stability studies
- Academic & contract research: Method development, forced degradation studies, and kinetic modeling of chromatographic separations
FAQ
Is this unit certified for GxP-regulated environments?
Yes—each refurbished Agilent 1260 Infinity II undergoes IQ/OQ verification and ships with documentation suitable for inclusion in validation protocols under FDA, EMA, and PMDA requirements.
What does the 1-year warranty cover?
The warranty covers parts and labor for defects in materials or workmanship under normal laboratory use; excludes consumables (seals, frits, lamps), damage from misuse, power surges, or unauthorized modification.
Can legacy 1200-series methods be run without modification?
Yes—hardware and software are fully backward-compatible; method transfer typically requires only minor dwell volume adjustment in the gradient program.
Is remote diagnostics supported?
Yes—when connected to a secure corporate network, Agilent Remote Assist enables authorized engineers to perform health checks, firmware updates, and troubleshooting with customer consent.
Are original Agilent service records available?
All units include complete service history from prior ownership, including maintenance logs, lamp usage hours, pump seal replacement dates, and most recent calibration certificates.
