Agilent 1100 Series HPLC System (Refurbished, Certified Pre-Owned)

Overview

The Agilent 1100 Series HPLC System is a robust, modular high-performance liquid chromatography platform originally engineered for method development, quality control, and routine analytical applications in regulated and research environments. Based on dual-piston reciprocating pump technology with pulse-dampened flow delivery, it delivers stable solvent gradients across a wide pressure range (up to 400 bar), supporting reversed-phase, normal-phase, ion-exchange, and size-exclusion separations. This certified pre-owned unit has undergone full functional validation—including leak testing, flow accuracy verification, gradient composition linearity assessment, baseline noise/stability evaluation, and retention time reproducibility checks—per Agilent’s Refurbished Equipment Quality Assurance Protocol. All critical wear components (seals, check valves, rotor seals, lamp housings) have been replaced; the UV-Vis detector lamp has been calibrated against NIST-traceable standards; and the system firmware is updated to the latest compatible revision (Rev. A.03.x or higher). It operates under ChemStation software (B.04.03 or later), enabling full instrument control, data acquisition, peak integration, and report generation.

Key Features

• Quaternary solvent delivery system with high-pressure mixing and real-time gradient programming (0.1–100% B, 0.1% increment)
• Autosampler with 100-position vial capacity, injection volume range 0.1–100 µL (with precision ≤ ±0.5% RSD at 10 µL)
• Column compartment with temperature control from ambient +5 °C to 80 °C (±0.5 °C stability)
• Variable-wavelength UV-Vis detector (190–600 nm), featuring deuterium and tungsten lamps, 1 nm resolution, and < 0.0005 AU/hr baseline drift
• Integrated online degasser (four-channel, membrane-based) ensuring consistent solvent saturation and minimized bubble formation
• Modular architecture supporting future expansion (e.g., addition of fluorescence or conductivity detectors, fraction collectors, or preparative modules)

Sample Compatibility & Compliance

The Agilent 1100 accommodates standard 3.9–4.6 mm ID analytical columns (up to 250 mm length) and is compatible with C18, C8, phenyl-hexyl, HILIC, and silica-based stationary phases. It supports mobile phases including aqueous buffers (e.g., phosphate, acetate, ammonium formate), organic modifiers (acetonitrile, methanol), and ion-pairing reagents. The system meets foundational requirements for GLP-compliant laboratories and aligns with key regulatory expectations outlined in USP , ICH Q2(R2), and ASTM E2623–19. While not inherently 21 CFR Part 11 compliant in its base configuration, ChemStation B.04.03+ supports optional audit trail activation, electronic signature modules, and user access level management—enabling qualification for GMP environments when deployed with documented IQ/OQ/PQ protocols.

Software & Data Management

Controlled via Agilent ChemStation for LC Systems (B.04.03 or newer), the platform provides intuitive method setup, real-time chromatogram display, automated peak detection and integration, calibration curve generation (linear, quadratic, weighted), and customizable reporting templates. Raw data (.D files) are stored in a hierarchical directory structure with embedded metadata (instrument parameters, sequence logs, user IDs). ChemStation supports export to CSV, PDF, and XML formats; integrates with LIMS via ODBC drivers; and permits secure remote monitoring through Agilent’s Web-Based Remote Access module (requires local network configuration). All software licenses included are perpetual and transferable per Agilent’s pre-owned equipment licensing terms.

Applications

This system is routinely deployed in pharmaceutical QC labs for assay and impurity profiling of APIs and finished dosage forms; in environmental labs for PAH, pesticide, and pharmaceutical residue analysis in water and soil extracts; in food safety testing for mycotoxin quantification (e.g., aflatoxins, ochratoxin A); and in academic research for natural product isolation, polymer characterization, and metabolomics sample preparation. Its proven reliability, broad method compatibility, and extensive literature support make it especially suitable for laboratories transitioning from legacy HPLC platforms or scaling up analytical throughput without capital-intensive investment.

FAQ

Is this unit eligible for extended warranty or service contracts?
Yes—Agilent-certified refurbished systems qualify for Agilent Care Extended Service Plans (ESP), including Preventive Maintenance Contracts and Priority Response Support.
Are original documentation and calibration certificates provided?
Yes—each unit ships with a full refurbishment dossier, including system test reports, lamp calibration certificates (NIST-traceable), and a signed Certificate of Conformance.
Can the system be integrated into an existing LabNetwork or connected to a central LIMS?
Yes—standard Ethernet connectivity (10/100BASE-T) and ChemStation’s ODBC interface enable seamless LIMS integration and centralized data archiving.
What consumables and spare parts are recommended for initial operation?
We recommend stocking seal kits (G1312-68705), purge valve assemblies (G1312-60012), UV lamp (G1314-60101), and column frits (5063-6591) prior to commissioning.
Is method transfer from other Agilent platforms supported?
Yes—ChemStation ensures backward compatibility with methods developed on Agilent 1050, 1100, and 1200 series instruments, minimizing revalidation effort.