Beifen Ruili UV-1801 Dual-Beam Ultraviolet-Visible Spectrophotometer (Refurbished)

Overview

The Beifen Ruili UV-1801 is a refurbished dual-beam ultraviolet-visible (UV-Vis) spectrophotometer engineered for precision absorbance, transmittance, and concentration measurements across research, quality control, and routine analytical laboratories. Based on the Beer–Lambert law, the instrument employs a split-beam optical architecture to simultaneously direct light through both sample and reference paths—enabling real-time compensation for source intensity drift, lamp aging, and environmental fluctuations. Its wavelength coverage spans 190 nm to 1100 nm, fully encompassing the far-UV, near-UV, visible, and near-infrared spectral regions critical for nucleic acid quantification (e.g., A₂₆₀/A₂₈₀), protein analysis (e.g., Bradford, Lowry), enzyme kinetics, dissolution testing, and colorimetric assays per USP , ISO 6223, and ASTM E275. The fixed 2 nm spectral bandwidth ensures compliance with pharmacopeial resolution requirements for identity and purity verification in pharmaceutical QC environments.

Key Features

• Dual-beam optical system with real-time background correction for enhanced photometric stability and measurement reproducibility
• Imported deuterium and tungsten-halogen lamps delivering stable output across full spectral range; lamp lifetime monitored and displayed via firmware
• High-sensitivity photodiode array detector with optimized signal-to-noise ratio and linear dynamic range of 0–3.0 A
• Motorized auto-scanning mechanism enabling programmable wavelength sweeps at selectable speeds (60–300 nm/min)
• Non-volatile memory retains up to 100 user-defined methods, calibration curves, and measurement datasets after power loss
• Expandable measurement modes including fixed-wavelength, time-based kinetics, multi-wavelength quantitation, derivative spectroscopy (1st–3rd order), dual/triple wavelength ratioing, and dedicated DNA/protein calculation protocols
• Modular interface support: standard RS-232 for legacy PC integration; optional RS-485 for multi-instrument daisy-chaining or USB for modern data logging

Sample Compatibility & Compliance

The UV-1801 accommodates standard 10 mm square cuvettes as well as extended-pathlength cells up to 100 mm—ideal for low-concentration samples or highly absorbing matrices. Its four-position manual sample holder supports rapid sequential analysis without re-alignment. Instrument design conforms to IEC 61010-1 safety standards for laboratory electrical equipment. All measurement functions—including calibration, validation, and data export—support GLP/GMP-aligned workflows. Audit trail functionality (when operated via compliant PC software) meets FDA 21 CFR Part 11 requirements for electronic records and signatures. Performance verification follows ISO/IEC 17025 principles and can be documented using NIST-traceable neutral density filters and holmium oxide reference standards.

Software & Data Management

The spectrophotometer operates independently via its integrated 6-inch blue LCD interface, supporting immediate absorbance, %T, and concentration readouts. For advanced analysis, optional PC-based software enables full instrument control, spectral overlay, peak identification, baseline correction, and kinetic curve fitting. Raw spectral data exports in CSV or ASCII format for post-processing in MATLAB, OriginLab, or Python-based analytical pipelines. All exported files include embedded metadata: timestamp, operator ID (if entered), instrument serial number, lamp hours, and calibration status. Data integrity is preserved via checksum-verified transfers and configurable auto-backup intervals.

Applications

• Pharmaceutical QC: Assay validation, excipient interference screening, and dissolution profile monitoring per USP
• Biotechnology: Quantification of dsDNA, ssDNA, RNA, and proteins using extinction coefficients; purity assessment via A₂₆₀/A₂₈₀ and A₂₆₀/A₂₃₀ ratios
• Environmental Testing: Nitrate, phosphate, and heavy metal speciation using colorimetric reagents (e.g., APHA Standard Methods)
• Food & Beverage: Color index determination (e.g., AOAC 990.15), antioxidant capacity (FRAP/DPPH), and preservative quantification
• Academic Teaching: Fundamental spectroscopy experiments, Beer’s law verification, and comparative analysis of chromophore behavior
• Clinical & Public Health: ELISA endpoint reading, bacterial growth monitoring, and serological assay development

FAQ

Is this unit factory-refurbished or dealer-certified?
This UV-1801 unit undergoes a comprehensive refurbishment protocol conducted by Beifen Ruili–authorized service centers, including optical alignment verification, wavelength accuracy recalibration against certified standards, lamp replacement, and full functional testing per QCP-023.
Does it include a certificate of calibration?
Yes—each unit ships with a traceable calibration report signed by an ISO/IEC 17025-accredited metrology lab, covering wavelength accuracy (±0.5 nm at 200/600/1000 nm), photometric linearity (0–2.5 A), and stray light performance.
Can it be integrated into a LIMS environment?
Via RS-232 or optional USB-to-serial adapter, the instrument supports ASCII command protocols compatible with common LIMS middleware (e.g., LabVantage, Thermo SampleManager) for automated result ingestion and audit-ready data routing.
What is the expected lamp lifetime under typical usage?
Deuterium lamp: ≥1,000 hours; tungsten-halogen lamp: ≥2,000 hours. Firmware displays cumulative lamp-on time and triggers maintenance alerts at 90% rated life.
Are cuvettes and accessories included?
Standard quartz and UV-grade glass cuvettes (10 mm pathlength) are available as optional add-ons. The base configuration includes one pair of matched 10 mm quartz cells and a dust cover.