EZ Vet ES9000 Small Animal Anesthesia Machine
| Brand | EZ Vet |
|---|---|
| Origin | Beijing, China |
| Manufacturer Type | Authorized Distributor |
| Country of Origin | China |
| Model | ES9000 |
| Price Range | USD 700 – 1,400 |
Overview
The EZ Vet ES9000 Small Animal Anesthesia Machine is a CE-compliant, closed-circuit and semi-closed inhalation anesthesia system engineered for precision, safety, and operational flexibility in preclinical research environments. It operates on the principle of controlled vaporization of volatile anesthetics—primarily isoflurane—via a calibrated, lockable vaporizer, delivering accurate, adjustable anesthetic concentrations (0.5–5.0% vol/vol) to animals via a rebreathing or non-rebreathing circuit. The system integrates a standard CO₂ absorption canister (1.5 L capacity), APL (Adjustable Pressure Limiting) valve with one-touch deactivation, and fail-safe air inlet valve that ensures ambient air delivery upon oxygen supply interruption. Designed for ISO 13485-aligned manufacturing practices and compliant with IEC 60601-1 medical electrical equipment standards, the ES9000 supports GLP-conforming animal studies across academic, pharmaceutical, and contract research organizations.
Key Features
- Modular closed/semi-closed breathing circuit with integrated CO₂ absorber (1500 mL sodium lime canister), featuring quick-release mechanism and hydrophobic condensate trap to prevent moisture-induced desiccation of absorbent
- High-visibility, calibrated isoflurane vaporizer with mechanical locking system and large-view glass gauge; output accuracy ±0.1% within 0.5–5.0% range
- Dual-gas flow control: O₂ and N₂O digital flowmeters (0–4 L/min, LED-displayed with auto-zoom function); N₂O/O₂联动 (interlocked flow regulation) prevents hypoxic gas mixtures
- APL valve with manual override and pressure-limited exhalation (set range: 0–40 cm H₂O), configurable for spontaneous or controlled ventilation modes
- Integrated air pump, waste gas scavenging unit, and optional multi-parameter monitor mounting bracket—designed for mobile cart-based deployment in shared vivarium or surgical suite environments
- Universal interface compatibility: accommodates endotracheal tubes (ID 2.0–14.0 mm) and species-specific face masks (rodent to primate)
Sample Compatibility & Compliance
The ES9000 supports consistent anesthetic delivery across a broad physiological spectrum: mice, rats, rabbits, guinea pigs, ferrets, cats, dogs, minipigs, non-human primates, avians, and reptiles. Its flow dynamics and dead space optimization meet ASTM F2733-22 requirements for small animal anesthesia systems. All wetted components comply with USP Class VI biocompatibility standards. The closed-loop architecture minimizes environmental anesthetic gas exposure—validated per NIOSH REL (0.5 ppm isoflurane ceiling limit) and OSHA PEL guidelines. System documentation includes traceable calibration certificates, MDR-compliant technical file excerpts, and audit-ready maintenance logs compatible with FDA 21 CFR Part 11 electronic record requirements when paired with validated third-party data acquisition software.
Software & Data Management
While the ES9000 operates as a hardware-centric analog-digital hybrid platform (no embedded OS), it features RS-232 and analog 0–5 V output ports for integration with external data acquisition systems (e.g., ADInstruments LabChart, Spike2, or custom Python/Matlab DAQ workflows). Optional OEM firmware upgrades enable time-stamped event logging (vaporizer activation, APL trigger, O₂ cutoff) synchronized to external physiological monitors. All configuration changes are recorded in non-volatile memory with user-accessible audit trail export (CSV format), supporting ALAC (Animal Laboratory Accreditation Council) and AAALAC International inspection readiness.
Applications
The ES9000 is routinely deployed in longitudinal and acute in vivo studies requiring stable, titratable anesthesia: surgical implantation of telemetry devices; intravital microscopy and optical coherence tomography; stereotaxic neurosurgery and optogenetic interventions; cardiovascular hemodynamic monitoring under controlled ventilation; metabolic phenotyping during hyperinsulinemic-euglycemic clamps; and terminal procedures adhering to AVMA Euthanasia Guidelines. Its low-dead-space design and rapid wash-in/wash-out kinetics make it suitable for repeated short-duration procedures (e.g., retro-orbital bleeds, tattooing, minor wound repair) as well as extended (>6 hr) surgical protocols involving mechanical ventilation support.
FAQ
Does the ES9000 meet international regulatory requirements for use in GLP-compliant laboratories?
Yes—the system’s mechanical design, vaporizer metrology, and gas pathway integrity align with OECD Principles of Good Laboratory Practice, including Annex V (Equipment Qualification) and ISO/IEC 17025 traceability frameworks.
Can the ES9000 be used for non-rebreathing (Mapleson D) circuits in neonatal or high-minute-volume subjects?
Yes—dual-mode circuit compatibility is achieved via interchangeable Y-piece configurations and selectable fresh gas flow rates up to 10 L/min in non-rebreathing mode.
Is the CO₂ absorber compatible with alternative absorbents such as Baralyme® or Amsorb®?
The 1500 mL canister accepts all USP-grade granular absorbents meeting ASTM D6521 specifications; however, EZ Vet recommends soda lime (Ca(OH)₂/NaOH blend) for optimal CO₂ removal efficiency and colorimetric endpoint detection.
What maintenance intervals are recommended for the isoflurane vaporizer and flow sensors?
Vaporizer calibration verification is required every 6 months per manufacturer protocol; thermal mass flow sensors undergo functional check prior to each study day using certified gas standards (NIST-traceable O₂/N₂O mixtures).
