YG-1000 Cylindrical Pressure-Driven Sterile Filtration System
| Origin | Zhejiang, China |
|---|---|
| Manufacturer Type | Authorized Distributor |
| Origin Category | Domestic (China) |
| Model | YG-1000 |
| Price | USD 195 (FOB) |
| Maximum Operating Pressure | 0.6 MPa |
| Test Pressure for Flow Rate | 0.2 MPa |
| Membrane Pore Size (for flow rating) | 0.22 µm |
| Capacity | 1000 mL |
| Compatible Filter Disc Diameter | Φ100 mm |
| Inlet Port Diameter | 6 mm |
| Outlet Port Diameter | 7.8 mm |
| Vertical Height from Outlet to Work Surface | 18 cm / 27 cm (adjustable) |
| Total Height | 45 cm |
| Water Flow Rate | 0.7 L/min |
Overview
The YG-1000 Cylindrical Pressure-Driven Sterile Filtration System is a benchtop, stainless-steel filtration unit engineered for reliable, repeatable sterile and clarifying filtration under controlled positive pressure. Designed in accordance with fundamental principles of cross-flow and dead-end membrane filtration, the system operates via externally applied gas pressure—typically nitrogen or compressed air—to drive liquid samples through certified microporous membranes. Its cylindrical housing geometry ensures uniform pressure distribution across the filter surface, minimizing channeling and maximizing membrane utilization. The device is not a vacuum-driven unit; rather, it functions exclusively as a pressurized filtration assembly compliant with standard laboratory-scale aseptic processing workflows. It is intended for use in environments requiring reproducible, low-particulate, non-denaturing filtration—including preparation of sterile culture media, clarification of biological therapeutics, and pre-filtration of analytical samples prior to HPLC or cell-based assays.
Key Features
- Robust construction using electropolished 304 or optional 316L stainless steel—ensuring corrosion resistance against common solvents, buffers, and sterilizing agents (e.g., 0.1 M NaOH, 70% ethanol, saturated steam)
- Smooth internal surface finish (Ra ≤ 0.4 µm) to prevent microbial adhesion and facilitate cleaning-in-place (CIP) protocols
- Modular, tool-free disassembly for rapid membrane replacement and validation-compatible inspection
- Pressure-rated housing certified for continuous operation up to 0.6 MPa (87 psi), with integrated pressure relief mechanism
- Two fixed outlet height configurations (18 cm and 27 cm) to accommodate varied receiver vessel sizes while maintaining ergonomic workflow alignment
- Standardized Φ100 mm filter disc interface compatible with commercially available sterile-grade membranes (0.1 µm, 0.22 µm, 0.45 µm, and 1.2 µm pore sizes) from leading suppliers including Pall, Sartorius, and Merck Millipore
Sample Compatibility & Compliance
The YG-1000 supports aqueous and mildly organic solutions commonly encountered in bioprocessing and pharmaceutical labs—including cell culture media, serum-supplemented formulations, protein-containing buffers, and low-viscosity therapeutic eluates. It is not suitable for high-solids suspensions (>0.5% w/v), viscous polymer solutions (e.g., >10 cP at 25°C), or aggressive oxidizers (e.g., undiluted hydrogen peroxide). While the unit itself carries no regulatory certification (e.g., FDA 510(k), CE-IVD), its materials comply with USP Class VI biocompatibility requirements, and its design permits integration into GLP- and GMP-aligned processes when paired with qualified membranes and documented operational procedures. Users are responsible for validating filter integrity (via bubble point or diffusion test) and process consistency per ISO 13408-1 and ASTM F838-22 standards.
Software & Data Management
The YG-1000 is a manually operated, analog filtration system with no embedded electronics, firmware, or digital connectivity. It requires external pressure regulation via calibrated needle valve or regulated gas supply. As such, it does not generate electronic records and is inherently compliant with environments where data integrity concerns preclude automated instrumentation—for example, in early-stage process development labs operating under ALCOA+ principles without 21 CFR Part 11-compliant audit trails. All operational parameters (inlet pressure, filtration time, volume processed) must be recorded manually in lab notebooks or LIMS-integrated paper logs. This architecture eliminates cybersecurity risks and reduces qualification burden during facility audits.
Applications
- Sterile filtration of heat-labile culture media (e.g., DMEM, RPMI-1640) prior to cell line expansion
- Clarification of monoclonal antibody harvests and viral vector preparations in academic and contract development labs
- Final filtration of ophthalmic and injectable pharmaceutical solutions under ISO Class 5 hood conditions
- Pre-filtration of serum, plasma, or ascites fluid prior to ELISA or mass spectrometry analysis
- Removal of mycoplasma and bacteria from enzyme stock solutions used in CRISPR editing workflows
FAQ
Can the YG-1000 be autoclaved?
Yes—the entire assembled unit (including lid, base, and support ring) is rated for repeated steam sterilization at 121°C for 20 minutes. Ensure all O-rings are silicone-grade and verify torque specifications before each cycle.
Is a pressure regulator included with the system?
No. A precision pressure regulator (0–0.6 MPa range, ±0.01 MPa accuracy) and compatible gas tubing must be sourced separately to ensure safe and reproducible operation.
What validation documentation is provided?
A material compliance certificate (304/316L SS traceability), dimensional drawing, and maximum pressure rating test report are supplied. Full IQ/OQ/PQ protocols are user-defined and must align with site-specific validation master plans.
Can I use track-etched polycarbonate membranes with this unit?
Yes—provided the membrane diameter is Φ100 mm and the support grid is compatible with the unit’s stainless-steel filter holder. Note that hydrophobic membranes require pre-wetting with alcohol prior to aqueous filtration.
