Qiangle HZL-X100 Dual-Chamber Constant-Temperature Orbital Incubator Shaker

Overview

The Qiangle HZL-X100 Dual-Chamber Constant-Temperature Orbital Incubator Shaker is an integrated life science platform engineered for simultaneous temperature-controlled incubation and uniform orbital agitation of biological samples. Designed specifically for microbiological culture expansion, cell suspension maintenance, enzyme reaction kinetics, and aerobic microbial growth studies, the system employs a dual-layer vertical architecture to maximize workspace efficiency without compromising thermal or mechanical stability. Its core operation relies on precision PID-controlled heating elements combined with forced-air convection and a low-vibration brushless DC drive mechanism, ensuring consistent orbital motion (25 mm diameter) across the full speed range (30–300 rpm). The chamber maintains temperature uniformity within ±0.5 °C at setpoints between 0 °C and 60 °C — a performance benchmark aligned with ISO 13485-compliant environmental control requirements for routine QC microbiology workflows.

Key Features

• Dual-layer vertical design offering 100 L total internal volume with independent access doors for upper and lower chambers — optimized for parallel experiments or staged incubation protocols.
• Intelligent microprocessor controller with high-resolution LED display, real-time parameter monitoring (temperature, speed, elapsed time), and non-volatile memory for up to 10 user-defined programs.
• Brushless DC motor drive delivering high torque at low speeds, extended service life (>20,000 hours MTBF), and minimal electromagnetic interference — critical for adjacent sensitive instrumentation.
• Forced-air circulation system with rear-mounted centrifugal fan and precisely positioned baffles to ensure uniform heat distribution and eliminate cold/hot spots per ASTM E1751-22 guidelines for incubator validation.
• Integrated humidity control (20–60 % RH) via adjustable water reservoir and passive evaporation management — suitable for non-sterile, moisture-sensitive cultures requiring moderate ambient humidity.
• Comprehensive safety architecture including independent over-temperature cutoff (mechanical backup), motor overload detection, door-open interlock, and audible/visual alarm indicators.

Sample Compatibility & Compliance

The HZL-X100 accommodates standard laboratory vessels including Erlenmeyer flasks (up to 2 L), test tubes (15–50 mL), multi-well plates (deep-well format), and culture bottles — all secured via universal spring-loaded clamps compatible with round-bottom and flat-bottom configurations. It supports common microbiological media (e.g., LB, TSB, MRS), mammalian suspension cultures (e.g., CHO, Sf9), and enzymatic assay buffers. While not certified for sterile operation or ISO Class 5 environments, the unit meets general-purpose GLP laboratory requirements for environmental monitoring and non-aseptic cultivation. Its temperature control accuracy and repeatability comply with ISO/IEC 17025 clause 5.4.2 for equipment used in accredited testing laboratories. Documentation includes factory calibration certificates traceable to NIM (National Institute of Metrology, China).

Software & Data Management

The HZL-X100 operates as a standalone instrument with embedded firmware; no external PC software is required for basic operation. However, optional RS-232 serial interface enables connection to LIMS or lab automation systems for remote parameter logging and event-triggered data capture. All operational parameters (setpoint, actual temperature, speed, runtime) are logged internally with timestamping and retained for ≥30 days. Audit trail functionality supports GLP/GMP-aligned record retention, including operator ID entry (via numeric keypad), parameter change history, and alarm event logs — fully compliant with FDA 21 CFR Part 11 principles when paired with validated third-party data acquisition software.

Applications

• Microbial growth curve analysis under controlled thermal and agitation conditions for antibiotic susceptibility testing (AST) and bioburden assessment.
• Suspension cell culture propagation for transient transfection studies and recombinant protein expression in shake-flask bioreactors.
• Enzyme kinetics assays requiring constant mixing and precise temperature stabilization (e.g., lipase activity, polymerase fidelity studies).
• Environmental microbiology applications including BOD incubation, soil respiration assays, and wastewater microbial community enrichment.
• Educational use in undergraduate and graduate teaching labs for hands-on instruction in fermentation dynamics and environmental physiology.

FAQ

Is the HZL-X100 suitable for CO₂-dependent cell cultures?
No — this model does not include CO₂ regulation or gas-tight sealing; it is intended for ambient-air aerobic or facultative anaerobic applications only.
Can the humidity function be disabled independently of temperature control?
Yes — the humidity module operates separately and can be turned off while maintaining full temperature and shaking functionality.
What is the maximum flask size supported on each shelf?
Each chamber layer accepts up to four 2 L Erlenmeyer flasks or sixteen 500 mL flasks using the standard universal clamp tray configuration.
Does the unit include validation documentation for IQ/OQ protocols?
Factory-provided IQ/OQ templates and calibration reports are included; final PQ must be performed by the end-user per local SOPs and ISO/IEC 17025 Annex A.3.
Is remote monitoring via Ethernet or Wi-Fi available?
Not natively — only RS-232 serial communication is provided; Ethernet/Wi-Fi integration requires external gateway hardware and custom middleware development.