Qiangle LSHZ-300 Cryogenic Water Bath Shaker

Overview

The Qiangle LSHZ-300 Cryogenic Water Bath Shaker is an engineered platform for precise temperature-controlled agitation of biological and chemical samples under sub-ambient conditions. Designed around a dual-mode thermal architecture—integrating a high-efficiency hermetic compressor (280 W) with a recirculating glycol–water coolant loop—the system achieves stable operation from –10 °C to 99 °C without external chiller dependency. Unlike conventional water baths limited to ambient-plus heating, the LSHZ-300 supports cryogenic incubation protocols essential for enzyme kinetics, cold-adapted microbial culture, protein stability assays, and low-temperature reaction monitoring. Its horizontal orbital motion (25 mm amplitude, fixed-speed or variable 40–280 rpm control) ensures uniform suspension and minimal shear stress across all 14 × 250 mL positions on the single stainless-steel shaker platform. The unit complies with IEC 61010-1 safety standards for laboratory equipment and incorporates over-temperature, over-current, and compressor overload protection circuits.

Key Features

• Integrated refrigeration system enabling true sub-zero operation (–10 °C minimum) without auxiliary chillers
• Wide dynamic temperature range (–10 °C to 99 °C) with ±0.3 °C thermal stability at setpoint (measured at center bath position, steady-state, n=3)
• Digitally adjustable oscillation frequency (40–280 rpm) with closed-loop motor feedback for consistent rotational accuracy
• Single-tier 304 stainless-steel platform rated for 14 × 250 mL Erlenmeyer flasks or compatible vessels (max load: 8 kg distributed)
• Robust thermal insulation (polyurethane foam, 45 mm thickness) minimizing energy loss and condensation formation
• Front-panel LED display with independent controls for temperature setpoint, speed, and operational status indicators
• Compressor cooling circuit designed for continuous duty cycle (MTBF > 25,000 hours per ISO 13374-2)

Sample Compatibility & Compliance

The LSHZ-300 accommodates standard microbiological and biochemical vessels—including glass and polycarbonate flasks, test tubes (up to 50 mL), and multi-well plates secured via optional clamping accessories. Its bath chamber volume (≈12 L) supports full immersion of 250 mL flasks with adequate headspace for aeration. The system meets CE marking requirements under Directive 2014/30/EU (EMC) and 2014/35/EU (LVD). It is suitable for use in GLP-compliant laboratories when operated with documented calibration procedures (per ISO/IEC 17025) and routine verification using NIST-traceable PT100 probes. While not inherently 21 CFR Part 11 compliant, audit-ready operation is achievable through external data logging systems with electronic signature capability.

Software & Data Management

The LSHZ-300 operates as a standalone instrument with no embedded microprocessor-based data logging. Temperature and speed parameters are set manually via front-panel controls. For traceable process documentation, users may integrate third-party USB or RS-485 data loggers (e.g., Omega OM-DAQPRO-5300 series) connected to the analog output terminals (0–5 V proportional to actual bath temperature and rpm). Calibration certificates for factory-installed PT100 sensors are provided with each unit. Firmware updates are not applicable; hardware-level thermal and motion calibrations are performed pre-shipment and verified annually per user-maintained maintenance logs.

Applications

• Cold-shock induction of recombinant protein expression in E. coli strains
• Long-term viability studies of psychrophilic bacteria and archaea
• Thermal denaturation profiling of monoclonal antibodies below physiological temperatures
• Solubility and crystallization screening of small molecules at controlled sub-zero gradients
• Extraction efficiency optimization for thermolabile natural products (e.g., anthocyanins, glucosinolates)
• Standardized QC testing of temperature-sensitive reagents (e.g., restriction enzymes, ligases, RT-PCR master mixes)

FAQ

Does the LSHZ-300 include a built-in timer function?
No. The unit lacks internal timing circuitry. Users requiring timed operation must employ an external programmable timer relay or integrate with a PLC-controlled lab automation system.

What coolant mixture is recommended for operation below 0 °C?
A 30% v/v aqueous solution of USP-grade propylene glycol is validated for –10 °C performance and corrosion inhibition. Ethylene glycol is not recommended due to toxicity concerns in shared lab environments.

Can the shaker accommodate flask clamps for 500 mL vessels?
The standard platform supports up to 250 mL flasks at rated capacity. Custom clamp kits for larger vessels (up to 500 mL) are available as optional accessories—contact technical support for mechanical compatibility verification.

Is the compressor serviceable on-site by authorized technicians?
Yes. All critical refrigeration components—including the sealed compressor, expansion valve, and evaporator coil—are accessible via rear-panel service ports. Qiangle-certified field engineers perform preventive maintenance per ISO 55001-aligned asset management schedules.