RWD D165H/D165HT Gas-Jacketed CO₂ Incubator
| Brand | RWD |
|---|---|
| Origin | Shenzhen, China |
| Manufacturer Type | Direct Manufacturer |
| Instrument Type | Gas-Jacketed CO₂ Incubator |
| CO₂ Detection | Infrared (IR) Sensor |
| Temperature Control Range & Accuracy | ±0.1 °C at 37 °C |
| Temperature Uniformity | ±0.25 °C at 37 °C |
| CO₂ Concentration Control Range & Accuracy | 0–20% with ±0.1% accuracy at 5% setpoint |
| Internal Chamber Capacity | 3.8 L |
| Internal Dimensions (W×D×H) | 47.0 × 57.0 × 60.0 cm |
Overview
The RWD D165H/D165HT is a compact, gas-jacketed CO₂ incubator engineered for precision cell culture in demanding life science applications—including primary cell isolation, stem cell expansion, and embryology workflows. Its core architecture employs independent gas jacket heating combined with IR-based CO₂ sensing and optional O₂ monitoring to maintain tightly regulated tri-gas environments (CO₂, O₂, N₂). Unlike water-jacketed systems, the gas-jacket design ensures rapid thermal recovery and eliminates condensation risks while delivering exceptional temperature stability (±0.1 °C at 37 °C) and uniformity (±0.25 °C across the chamber). The incubator supports physiologically relevant hypoxic conditions—critical for modeling in vivo microenvironments—via an optional zirconia-based O₂ sensor module calibrated for long-term drift resistance and repeatability.
Key Features
- 180 °C Dry-Heat Sterilization Cycle: Validated to achieve ≥12-log reduction in microbial bioburden (SAL ≤10⁻¹²), including bacterial endospores, fungi, and molds—per ISO 14937 and EN 556-1. Full cycle completes in ~12 hours; requires only HEPA filter removal—no disassembly of sensors, fans, or shelves.
- Integrated 3-L Reservoir Humidification System: Delivers humidity recovery to >95% RH within 10 minutes after a 30-second door opening—5× faster than conventional water pan designs. Real-time water level monitoring triggers low-humidity mode to suppress condensate formation and microbial proliferation.
- Tri-Gas Capability (Optional O₂ Module): Zirconia electrochemical O₂ sensor enables stable, trace-level oxygen control (0.1–20% O₂) with <±0.1% accuracy. Supports normoxic, hypoxic, and anoxic culture protocols aligned with USP , ISO 13485, and GLP-compliant workflows.
- Three-Panel Inner Door Design: Minimizes chamber air exchange by up to 67% per access event versus full-width doors. Reduces thermal and CO₂ fluctuations, lowers energy consumption, and maintains sterility integrity during routine handling.
- R-Link Cloud-Based Control System: Web-enabled platform supporting remote monitoring, alarm push notifications (SMS/email), and audit-trail logging compliant with FDA 21 CFR Part 11 requirements—including electronic signatures, immutable operation logs, and role-based access (Admin/Operator/Guest).
Sample Compatibility & Compliance
The D165H/D165HT accommodates standard tissue culture vessels—including T25/T75 flasks, 6-/12-/24-well plates, and Petri dishes—within its 3.8 L internal volume. Its stainless-steel interior (electropolished AISI 304), copper-enriched fan housing, and HEPA-filtered recirculation airflow (≥99.99% @ 0.3 µm) meet ISO 14644-1 Class 5 cleanroom equivalency. The system complies with IEC 61010-1 (safety), IEC 61326-1 (EMC), and EN 60601-1 (medical device interoperability). Sterilization validation reports and IQ/OQ documentation are available upon request for GMP/GLP-regulated laboratories.
Software & Data Management
R-Link firmware implements secure TLS 1.2 encrypted communication and stores all operational data locally on an industrial-grade SD card (16 GB) with automatic rollover. Logged parameters include chamber temperature, CO₂/O₂ concentrations, humidity, door status, sterilization cycle timestamps, and user authentication events. Audit trails retain metadata (user ID, timestamp, action, pre/post values) for ≥10 years. Data export supports CSV and PDF formats compatible with LIMS integration. Role-based permissions enforce separation of duties: Admins configure settings and manage users; Operators execute runs; Guests view real-time metrics only.
Applications
- Primary human cell culture under physiological O₂ (1–5%) to improve viability and differentiation fidelity
- Embryonic stem cell maintenance requiring strict CO₂ (5%) and O₂ (2–8%) control
- Microbiological co-culture studies involving anaerobic or microaerophilic bacteria
- Regulatory-compliant stability testing per ICH Q5C guidelines
- High-value sample storage where environmental continuity is critical (e.g., CAR-T manufacturing support)
FAQ
Does the D165H/D165HT support FDA 21 CFR Part 11 compliance?
Yes—R-Link enforces electronic signatures, audit-trail immutability, and role-based access controls required for Part 11 adherence.
What is the maximum allowable O₂ concentration range when the optional module is installed?
The zirconia sensor supports 0.1% to 20% O₂ with ±0.1% accuracy across the full span.
Can the 180 °C sterilization cycle be validated internally using biological indicators?
Yes—the cycle is compatible with standard Geobacillus stearothermophilus spore strips (e.g., ATCC 7953); third-party validation reports are provided with each unit.
Is the humidity reservoir accessible without removing shelves or disturbing cultures?
Yes—refill and drainage are performed via front-panel quick-connect ports; no shelf repositioning is required.
How does the gas-jacket design improve temperature recovery compared to water-jacketed incubators?
Gas jackets eliminate thermal mass lag; combined with PID-controlled heating elements, they achieve <5-minute recovery to 37 °C after a 30-second door opening.
