Revvity Cellometer X2 Dual-Fluorescence Cell Counter
| Brand | Revvity |
|---|---|
| Origin | USA |
| Manufacturer | Revvity, Inc. |
| Product Type | Imported Instrument |
| Model | Cellometer X2 |
| Price | Contact for Quote |
Overview
The Revvity Cellometer X2 is a dual-fluorescence automated cell counter engineered for high-precision, rapid quantification and viability assessment of heterogeneous cell populations. It operates on the principle of digital image-based cytometry, combining brightfield microscopy with two independent fluorescence excitation/emission channels (typically 488 nm/520 nm for FITC/GFP and 532 nm/585 nm for PI or 7-AAD). Unlike flow cytometers requiring hydrodynamic focusing or dilution-based hemocytometers lacking morphological context, the Cellometer X2 captures high-resolution static images of cells immobilized on disposable counting slides—enabling simultaneous morphometric analysis (cell diameter, circularity, clump detection) and fluorescence intensity quantification per cell. Its 10× objective lens delivers an effective pixel resolution of ~1.6 µm, making it uniquely suited for sub-20 µm biological particles including platelets, yeast, microalgae, and bacterial aggregates—without reliance on centrifugation or staining optimization beyond standard protocols.
Key Features
- Dual-channel fluorescence imaging system with independently controlled LED excitation sources and bandpass-filtered detection paths for simultaneous live/dead discrimination using standard dyes (e.g., acridine orange/propidium iodide or calcein-AM/ethidium homodimer)
- Automated image acquisition and analysis powered by Revvity’s proprietary Cellometer Vision software—capable of distinguishing viable from non-viable cells based on membrane integrity and intracellular esterase activity
- Integrated 10× objective lens with fixed working distance and optimized depth of field to ensure consistent focus across slide chambers, minimizing user-induced variability
- Disposable precision-molded counting slides with defined chamber volume (20 µL), eliminating pipetting errors and cross-contamination risks associated with reusable hemocytometers
- Onboard calibration verification via NIST-traceable fluorescent microspheres; system performance logs are stored with each run for GLP-compliant recordkeeping
- Compact benchtop footprint (29 × 34 × 28 cm) with integrated touchscreen interface—no external PC required for routine operation
Sample Compatibility & Compliance
The Cellometer X2 supports a broad range of suspension-based biological samples, including mammalian cell lines (CHO, HEK293), primary human PBMCs, yeast (Saccharomyces cerevisiae, Pichia pastoris), microalgae (Chlamydomonas, Nannochloropsis), bacterial cultures (E. coli, Bacillus subtilis), and platelet-rich plasma. It complies with ISO 21530:2020 (Biotechnology — Cell counting — Principles and methods) and supports audit-ready workflows aligned with FDA 21 CFR Part 11 requirements when used with validated software configuration and electronic signature modules. All counting slides are manufactured under ISO 13485-certified conditions and supplied sterile and pyrogen-free.
Software & Data Management
Cellometer Vision v4.x provides intuitive workflow-driven operation—from sample loading guidance to final report generation. Each analysis session automatically records metadata including date/time, operator ID, slide lot number, exposure settings, and fluorescence gain values. Quantitative outputs include total cell count, viable/non-viable counts, concentration (cells/mL), mean cell diameter (µm), coefficient of variation (CV%) for size distribution, and viability percentage. Data export options include CSV, PDF, and XML formats compatible with LIMS integration. For regulated environments, optional software validation packages include IQ/OQ documentation, electronic audit trail activation, and role-based access control.
Applications
- Bioprocess development: Monitoring yeast and microbial culture viability during fermentation scale-up in synthetic biology and biofuel production
- Cell therapy QC: Rapid release testing of CAR-T and NK cell products prior to infusion, per USP & Ph. Eur. monographs on cell viability assays
- Food safety microbiology: Enumeration of probiotic bacteria and spoilage organisms without colony-forming unit (CFU) lag time
- Environmental monitoring: Quantification of phytoplankton and cyanobacteria in wastewater and aquaculture systems
- Academic research: High-throughput screening of antimicrobial compounds using bacterial viability kinetics
FAQ
What types of fluorescent dyes are compatible with the Cellometer X2?
Standard amine-reactive (FITC, TRITC), nucleic acid-binding (PI, DAPI, Hoechst), and enzymatic substrates (calcein-AM, CFDA-SE) are supported—provided their excitation/emission profiles align with the instrument’s dual-channel optical filters.
Can the Cellometer X2 differentiate between cell types in mixed populations?
Yes—when combined with cell-type-specific fluorescent labeling (e.g., CD45-PE for leukocytes, GFP-tagged algae), the system enables semi-quantitative gating based on fluorescence intensity and size thresholds.
Is calibration required before each use?
No routine calibration is needed; however, daily performance verification using supplied fluorescent microsphere standards is recommended to maintain traceability and meet ISO 21530 compliance.
Does the system support GMP documentation requirements?
With optional software licensing and configured electronic signatures, the Cellometer X2 meets core elements of FDA 21 CFR Part 11 for electronic records and signatures in pharmaceutical manufacturing and clinical labs.
