HISTO Cool Eagle Histo-2335 Rotary Microtome for Paraffin Sectioning
| Brand | HISTO |
|---|---|
| Origin | Zhejiang, China |
| Manufacturer Type | Authorized Distributor |
| Country of Origin | China |
| Model | Histo-2335 |
| Price | USD 9,500 (FOB) |
| Section Thickness Range | 1–60 µm (1 µm increments from 1–9 µm |
| Horizontal Feed Travel | 30 mm |
| Vertical Stroke | 70 mm |
| Maximum Specimen Dimensions (W×H×D) | 50 mm × 66 mm × 30 mm |
| Specimen Retraction | 60 µm (user-disablable) |
| Specimen Orientation Adjustment | ±8° horizontal and ±8° vertical |
| Footprint (W×D×H, including handwheel and waste tray) | 413 mm × 616 mm × 305 mm |
| Net Weight (without accessories) | 34 kg |
Overview
The HISTO Cool Eagle Histo-2335 is a precision-engineered rotary microtome designed specifically for consistent, high-fidelity paraffin-embedded tissue sectioning in histopathology laboratories. It operates on the principle of controlled mechanical advancement of a specimen block against a stationary steel or disposable blade, enabling reproducible thin-section generation under ambient or cooled conditions. Engineered for daily clinical and research workflows, the Histo-2335 integrates ergonomic refinement with metrological rigor—delivering sub-micron repeatability in thickness control while maintaining ISO 13485-aligned manufacturing traceability. Its rigid cast-aluminum base, low-vibration feed mechanism, and zero-backlash gear train ensure minimal section compression and knife chatter—critical for preserving cellular architecture in H&E, IHC, and special stain preparations.
Key Features
- Precision thickness calibration system with dual-scale vernier dial: enables intuitive selection across three resolution tiers (1 µm, 2 µm, 5 µm), optimized for routine diagnostics (4–5 µm), serial sectioning (1–3 µm), and semi-thin applications (up to 60 µm).
- Patented force-compensated handwheel drive: eliminates torque variation across the full feed range, ensuring smooth, fatigue-free operation even during extended sectioning sessions.
- Dual safety interlock system: mechanical detent lock and spring-loaded release prevent unintended specimen advancement during blade changes or maintenance.
- Full-length knife-edge utilization via lateral knife holder translation: three preset positions (left/center/right) align with standard paraffin cassette dimensions (e.g., 46 mm × 66 mm), maximizing blade service life and reducing edge wear asymmetry.
- Integrated specimen retraction (60 µm, user-deactivatable): minimizes compression artifact during block advance and supports ribbon formation for consecutive sections.
- Ergonomic specimen orientation stage: ±8° horizontal and vertical fine adjustment enables precise alignment of oblique or irregularly oriented tissues without auxiliary jigs.
- Red-coded blade guard and elastomeric blade retractor: comply with IEC 61000-1-2 occupational safety guidelines; fully enclose the cutting edge when not in active sectioning mode.
- Deep-draw magnetic waste tray (capacity >120 mL): captures ribbons and debris without overflow, facilitating rapid cleanup between specimens.
- Tool-free universal knife holder: accepts standard steel knives (e.g., Feather S35, Leica 819) and disposable blades (e.g., Sakura D35, Thermo Scientific Cryo 35), with integrated finger guard and lateral stability pins.
Sample Compatibility & Compliance
The Histo-2335 accommodates standard paraffin-embedded tissue blocks up to 50 mm (W) × 66 mm (H) × 30 mm (D), compatible with all major embedding cassettes (e.g., Sakura Tissue-Tek, Milestone HyperClarity). Optional fixed-specimen clamps support rapid sectioning of standardized blocks (46 mm × 66 mm × 10 mm) without manual positioning. The instrument conforms to CE marking requirements under Directive 2014/34/EU (ATEX) for laboratory equipment and meets essential health and safety requirements outlined in EN 61000-6-2 (EMC immunity) and EN 61000-6-4 (EMC emissions). All plastics and surface coatings are RoHS-compliant and non-outgassing per ISO 10993-5 cytotoxicity testing. Documentation includes full traceable calibration records aligned with GLP audit trails.
Software & Data Management
While the Histo-2335 is a manually operated mechanical microtome (no embedded electronics or digital interface), its design supports integration into regulated environments through documented operational procedures. Each unit ships with a factory-verified calibration certificate referencing NIST-traceable micrometer standards. Optional accessories include a digital thickness verification kit (certified optical comparator, ±0.2 µm uncertainty) and an electronic logbook template compliant with FDA 21 CFR Part 11 requirements for manual instrument use records—including operator ID, date/time stamps, thickness settings, and maintenance events. The machine’s mechanical simplicity ensures long-term reliability without firmware updates or software validation overhead.
Applications
- Routine histology: high-throughput sectioning of diagnostic biopsies and surgical resections at 4–5 µm for hematoxylin and eosin staining.
- Immunohistochemistry (IHC): ultra-consistent 3–4 µm sections minimizing antigen masking and improving antibody penetration uniformity.
- Serial section reconstruction: stable 1–2 µm sectioning for neuronal tracing, tumor margin mapping, and 3D histological modeling.
- Teaching laboratories: intuitive controls and visual zero-position reference (red alignment line) accelerate trainee competency development.
- Research pathology: compatibility with specialized blades and chilled stages supports cryo-paraffin hybrid protocols and lipid-rich tissue processing.
FAQ
Is the Histo-2335 compatible with frozen section workflows?
No—it is engineered exclusively for room-temperature paraffin sectioning. For cryosectioning, refer to the HISTO Cryo-2100 series.
Can the thickness increment be customized beyond the factory presets?
No—the 1/2/5 µm progression is mechanically indexed and non-adjustable to maintain ISO 8601 traceability and prevent user-induced calibration drift.
Does the instrument support automated ribbon collection?
Not natively; however, third-party ribbon collectors (e.g., Leica AutoCut, Sakura RibbonTrak) can be mounted via standard M4 threaded ports on the waste tray housing.
What maintenance intervals are recommended?
Lubrication of feed gears every 6 months (using Dow Corning 111 silicone grease); annual inspection of handwheel backlash and knife holder lateral play by authorized service personnel.
Is technical documentation available in English with regulatory references?
Yes—full bilingual (EN/CN) user manual, CE Declaration of Conformity, RoHS statement, and ISO 13485 manufacturing certificate are provided digitally and on physical media.
