Jiayuan JY92-II Ultrasonic Cell Disruptor
| Brand | Jiayuan |
|---|---|
| Origin | Beijing, China |
| Model | JY92-II |
| Ultrasonic Frequency | 20–25 kHz with automatic frequency tracking |
| Output Power | 650 W |
| Pulse Mode | Adjustable on-time (1–99 s) and off-time (1–99 s) |
| Cycle Count | 1–99 cycles |
| Standard Probe Diameter | Φ6 mm |
| Optional Probe Diameters | Φ2, Φ3, Φ8, Φ10, Φ15 mm |
| Sample Volume Range | 0.2–500 mL |
| Duty Cycle | 1–99% |
| Input Voltage | 220 V / 110 V, 50 Hz / 60 Hz |
Overview
The Jiayuan JY92-II Ultrasonic Cell Disruptor is a benchtop sonication system engineered for high-efficiency mechanical lysis of biological samples through controlled acoustic cavitation. Operating in the 20–25 kHz frequency range with real-time automatic frequency tracking, the instrument maintains optimal energy transfer across varying sample viscosities and compositions. Its 650 W nominal output power enables reproducible disruption of eukaryotic cells, prokaryotes, subcellular organelles, viral particles, and soft tissues—without thermal degradation when operated in pulsed mode. Unlike rotor-stator homogenizers or bead-beating systems, ultrasonic disruption delivers scalable, non-contact energy input directly into the liquid phase, making it particularly suitable for sensitive biomolecules such as membrane proteins, nucleic acids, and labile enzyme complexes.
Key Features
- Intelligent frequency tracking circuitry ensures stable resonance and consistent amplitude delivery despite load changes or probe wear.
- Pulse-mode operation with independently adjustable on-time (1–99 s), off-time (1–99 s), and cycle count (1–99) minimizes heat accumulation and preserves sample integrity.
- Duty cycle control (1–99%) allows fine-tuning of effective acoustic energy dosage per unit time—critical for optimizing yield and minimizing denaturation.
- Interchangeable titanium alloy probes (standard Φ6 mm; optional Φ2–Φ15 mm) support broad volume scalability—from microliter-scale microsamples to 500 mL batch processing.
- Robust aluminum-alloy transducer housing and sealed electronic compartment ensure long-term reliability under routine laboratory conditions.
- Front-panel digital interface with LED display provides real-time monitoring of operating parameters and error diagnostics.
Sample Compatibility & Compliance
The JY92-II accommodates diverse biological matrices including mammalian cell suspensions, bacterial cultures (e.g., E. coli, Bacillus spp.), yeast, plant protoplasts, tissue homogenates, and viral lysates. It supports preparation workflows compliant with common life science standards—including ISO 13485 pre-analytical processing guidelines and CLSI EP17-A2 recommendations for sample homogenization validation. While not certified for GMP manufacturing environments, its repeatable pulse programming and parameter logging capability align with GLP documentation requirements for academic and industrial R&D laboratories. The device meets IEC 61000-4 electromagnetic compatibility standards and conforms to GB/T 14710 environmental testing protocols for Class II medical-grade equipment.
Software & Data Management
The JY92-II operates as a standalone instrument without proprietary software dependency. All operational parameters are set and retained via embedded firmware, eliminating external PC linkage and associated cybersecurity concerns. Parameter settings—including power level, pulse sequence, and duty cycle—are stored in non-volatile memory and recalled at power-up. For traceability, users may manually log session data (time/date, probe ID, volume, protocol ID) in laboratory notebooks or LIMS-integrated spreadsheets. When deployed in regulated environments, the instrument’s deterministic behavior and absence of cloud connectivity facilitate audit readiness under FDA 21 CFR Part 11 principles—provided manual records are maintained with appropriate signature and version controls.
Applications
- Nucleic acid extraction: Efficient release of genomic DNA and total RNA from Gram-positive bacteria and fibrous plant tissues.
- Protein solubilization: Disruption of inclusion bodies and membrane fractions for downstream purification of recombinant proteins.
- Nanoparticle synthesis: Facilitation of sonochemical reduction and dispersion of metal oxides and polymeric nanocarriers.
- Cellular fractionation: Preparation of cytosolic, nuclear, and mitochondrial subfractions with minimal cross-contamination.
- Vaccine development: Inactivation and lysis of whole-pathogen vaccines while retaining antigenic epitope conformation.
- Phytochemical extraction: Enhanced recovery of thermolabile secondary metabolites (e.g., flavonoids, alkaloids) from botanical matrices.
FAQ
What is the maximum recommended continuous operating time per cycle?
Continuous operation beyond 3 minutes is not advised due to thermal buildup in the probe tip. Pulsed operation (e.g., 5 s on / 5 s off) is strongly recommended for sessions exceeding 2 minutes.
Can the JY92-II be used for sterilization or microbial inactivation?
No. While sonication induces microbial lysis, it does not provide validated sterilization efficacy per ISO 14937 or EN 556 standards. It is intended for sample preparation—not terminal sterilization.
Is probe calibration required before each use?
No formal calibration is specified by the manufacturer. However, visual inspection for tip erosion and periodic amplitude verification using a calibrated hydrophone (per ASTM E1002) is recommended for critical applications.
Does the instrument support external trigger input or TTL synchronization?
No. The JY92-II lacks digital I/O ports or external sync capability. Timing control is exclusively managed via front-panel interface.
What safety certifications does the unit hold?
It complies with GB 4793.1–2007 (equivalent to IEC 61010-1) for electrical safety in laboratory equipment and meets RoHS Directive 2011/65/EU restrictions on hazardous substances.
