Junyi JY-ECPT3000 High-Voltage Electrophoresis Power Supply

Overview

The Junyi JY-ECPT3000 is a high-voltage, microprocessor-controlled electrophoresis power supply engineered for precision, reproducibility, and operational flexibility in modern molecular biology laboratories. Designed specifically for demanding electrophoretic applications—including vertical slab gel electrophoresis, two-dimensional (2D) electrophoresis, isoelectric focusing (IEF), and DNA sequencing workflows such as SSR and AFLP marker analysis—the instrument delivers stable, programmable output across three independent control modes: constant voltage, constant current, and constant power. Its core architecture employs digital feedback regulation to maintain setpoint accuracy under variable load conditions, minimizing thermal drift and ensuring consistent migration kinetics across gels. The integrated temperature monitoring capability (RT+10°C to 65°C, optional) enables real-time thermal management during extended runs—critical for preserving protein integrity in IEF or preventing band distortion in high-voltage SDS-PAGE. Unlike basic benchtop supplies, the JY-ECPT3000 is built to support method-critical reproducibility required in GLP-compliant research environments and academic core facilities.

Key Features

• Triple-mode output control (constant voltage, constant current, constant power) with simultaneous real-time display of both setpoint and actual output values on a backlit 128×64-pixel LCD
• Wide dynamic range: 20–3000 V, 1–200 mA, and 1–200 W—suitable for standard agarose gels, high-resolution polyacrylamide gels, and low-conductivity native electrophoresis buffers
• High-resolution digital adjustment: 1 V, 1 mA, and 1 W increments ensure fine-grained method optimization and inter-laboratory protocol transferability
• Extended timer functionality (1 minute to 99 hours 59 minutes) supports overnight and multi-stage electrophoresis protocols without manual intervention
• Intelligent safety architecture featuring automatic shutdown upon open-circuit detection, overload protection, and ground-fault leakage sensing—compliant with IEC 61010-1 safety standards for laboratory electrical equipment
• Onboard memory stores up to 10 frequently used electrophoresis methods, enabling rapid recall and reducing setup errors
• Optional temperature-controlled operation mode integrates external thermistor feedback to dynamically adjust output and stabilize gel matrix temperature—essential for reproducible IEF and high-resolution protein separation

Sample Compatibility & Compliance

The JY-ECPT3000 is compatible with all standard electrophoresis systems requiring DC high-voltage excitation, including mini and large-format vertical electrophoresis tanks (e.g., Bio-Rad Mini-PROTEAN®, Thermo Fisher Owl E2 series), horizontal agarose systems, and custom-built IEF chambers. It supports common buffer chemistries (Tris-Glycine, Tris-Acetate, CAPS, and imidazole-based systems) and accommodates variable electrode configurations via four independent output port pairs. While not an FDA-cleared medical device, its design adheres to ISO/IEC 17025-aligned instrumentation practices for calibration traceability and operational consistency. The unit meets CE marking requirements under the Low Voltage Directive (2014/35/EU) and EMC Directive (2014/30/EU). For regulated environments, audit-ready features—including auto-log timestamps, method versioning, and non-volatile parameter storage—support alignment with GLP documentation standards and internal QA/QC procedures.

Software & Data Management

The JY-ECPT3000 operates as a standalone instrument with no proprietary PC software dependency; however, its programmable parameters and event-driven outputs are fully compatible with third-party laboratory information management systems (LIMS) via RS-232 serial interface (optional adapter). All user-defined methods—including voltage ramp profiles, stepwise timing sequences, and temperature thresholds—are stored in non-volatile memory with write-protection logic to prevent accidental overwrites. The device implements automatic parameter retention across power cycles and includes a configurable auto-shutdown function triggered by elapsed time or thermal threshold breach. While it does not provide native electronic audit trails per FDA 21 CFR Part 11, its deterministic behavior, repeatable output calibration, and timestamped method execution logs serve as foundational elements for lab-specific validation protocols and internal SOP documentation.

Applications

• Vertical SDS-PAGE and native PAGE for protein molecular weight estimation and conformational analysis
• Two-dimensional electrophoresis combining isoelectric focusing (IEF) in the first dimension and SDS-PAGE in the second
• High-resolution DNA fragment separation in denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE)
• Molecular marker analysis including SSR (simple sequence repeat), AFLP (amplified fragment length polymorphism), and RAPD profiling
• Preparative electrophoresis for downstream mass spectrometry or Western blotting where consistent field strength and thermal stability are critical
• Electroblotting and electroelution applications requiring precise voltage ramping and extended runtime reliability

FAQ

What electrophoresis tank models are compatible with the JY-ECPT3000?

The unit supports any DC-powered electrophoresis system with standard banana-jack or alligator-clip inputs, including Bio-Rad, Thermo Fisher, C.B.S. Scientific, and Hoefer tanks.
Does the JY-ECPT3000 include built-in temperature sensing?

No—the temperature control function requires an externally connected thermistor probe (sold separately); the instrument reads and regulates based on that input signal.
Can the timer be paused and resumed mid-run?

No—timing is continuous and non-interruptible; however, the auto-restart feature restores pre-failure settings after unexpected power loss.
Is calibration traceable to NIST or other national standards?

Calibration certificates are available upon request through authorized service centers; output verification follows ISO/IEC 17025 principles using calibrated multimeters and precision shunt resistors.
How many output channels can operate simultaneously?

All four output port pairs function independently but share the same power supply bus; simultaneous multi-tank operation is supported only if total current draw remains within 200 mA and combined power stays ≤200 W.