Hanuo SHZ-82AB Digital Display Refrigerated Air-Bath Orbital Shaker

Overview

The Hanuo SHZ-82AB Digital Display Refrigerated Air-Bath Orbital Shaker integrates precise temperature control with stable orbital agitation to support reproducible incubation of biological and biochemical samples under controlled thermal and mechanical conditions. Unlike water-bath shakers, this air-bath system employs forced convection via a high-efficiency fan and Peltier-assisted cooling (or resistive heating) to achieve rapid thermal equilibration across the chamber—enabling operation from ambient down to 0 °C without refrigerant compressors. Its orbital motion (20 mm diameter) generates gentle yet uniform mixing ideal for suspension cultures, microbial growth, enzyme assays, and protein expression studies where shear-sensitive cells or aggregates require minimal turbulence. Engineered for precision, the unit maintains ±0.1 °C temperature stability at setpoint and delivers speed control with digital feedback across its full 0–300 rpm range—critical for method transfer between labs and long-term stability testing per ISO/IEC 17025 guidelines.

Key Features

• Dual-mode temperature control: refrigerated cooling (0 °C minimum) and heating (up to 50 °C), calibrated to ±0.5 °C accuracy across the full operating range
• High-resolution digital display showing real-time speed (rpm) and chamber temperature (°C) with independent setpoint inputs
• Orbital shaking mechanism with balanced motor drive and low-vibration bearing assembly, ensuring consistent motion profile over extended duty cycles
• Stainless steel inner chamber (AISI 304) resistant to corrosion from aqueous buffers, ethanol, and mild disinfectants—compatible with GLP-compliant cleaning protocols
• Adjustable mechanical timer (0–120 minutes) with audible end-of-cycle alert; optional continuous operation mode
• Oxygen enrichment port integrated into chamber wall to support aerobic culture requirements during prolonged incubation
• Universal spring-loaded platform accommodates standard Erlenmeyer flasks (50–2000 mL), test tubes, petri dishes, and multi-well plates without adapter kits

Sample Compatibility & Compliance

The SHZ-82AB supports a broad spectrum of sample formats used in life science workflows—including liquid cultures (E. coli, yeast, mammalian cells), solid-phase immunoassays, antigen-antibody binding kinetics, and environmental microbiology enrichment broths. Its sealed air-bath architecture prevents condensation on vessel exteriors and eliminates cross-contamination risks associated with water baths. The device conforms to IEC 61010-1:2010 for safety in laboratory equipment and meets electromagnetic compatibility (EMC) requirements per EN 61326-1. While not certified for medical device manufacturing (ISO 13485), it is routinely deployed in QC/QA laboratories performing method validation under USP Analytical Instrument Qualification and adheres to general GMP principles for equipment suitability documentation.

Software & Data Management

This model operates as a standalone instrument with no embedded software or network interface. All operational parameters are configured manually via front-panel membrane keys and verified through the dual-line LCD. For audit-trail compliance in regulated environments, users may integrate external data loggers (e.g., Omega OM-DAQPRO-5300 series) connected to the analog output terminals (0–5 V DC proportional to temperature and speed). The absence of proprietary firmware simplifies 21 CFR Part 11 compliance when paired with validated third-party acquisition systems that provide electronic signatures, change controls, and secure archival—common practice in pharmaceutical stability studies per ICH Q5C.

Applications

• Microbial growth curve analysis under defined thermal and agitation conditions
• Plasmid amplification and recombinant protein expression in shake flasks
• Biochemical reaction optimization (e.g., ligase activity, restriction digests)
• Suspension cell passaging and viability assessment in bioprocess development
• Environmental sample enrichment (e.g., coliform detection per APHA Standard Methods 9221)
• Soil extract preparation and heavy metal leaching studies per EPA Method 1311
• Antibiotic susceptibility testing using broth microdilution formats

FAQ

Does the SHZ-82AB include refrigeration capability? Why is “Refrigerated” in the name?

Yes—the unit incorporates thermoelectric (Peltier) cooling to reach 0 °C without compressor-based refrigerants, enabling low-temperature incubation while maintaining compact footprint and low acoustic noise.
Is the temperature calibration traceable to NIST standards?

Factory calibration is performed using metrologically verified reference thermometers; users may perform in-house verification with a NIST-traceable RTD probe inserted via the chamber’s auxiliary port.
Can the shaker accommodate 2-L flasks?

Yes—the platform supports vessels up to 2000 mL when centrally loaded; maximum recommended load is 15 kg distributed evenly across the tray.
What maintenance is required for long-term reliability?

Annual inspection of fan airflow, motor brush wear (if applicable), and door gasket integrity is recommended; stainless steel chamber requires only routine wipe-down with 70% ethanol or isopropanol.
Is there an option for RS-232 or USB connectivity?

No—this is a manually operated instrument without digital communication ports. For automated data capture, external analog-to-digital interfaces must be used.