Hanuo SPE-12 Manual Solid Phase Extraction System

Overview

The Hanuo SPE-12 Manual Solid Phase Extraction System is a precision-engineered benchtop vacuum manifold designed for reproducible, scalable sample preparation in analytical laboratories. It operates on the fundamental principle of solid-phase extraction—selective adsorption, washing, and elution of analytes from complex liquid matrices using functionalized sorbent cartridges. Unlike automated platforms, the SPE-12 delivers full operator control over critical parameters including flow rate, solvent sequence, and vacuum application timing—making it especially suitable for method development, validation studies, and low-to-medium throughput applications where flexibility and cost-efficiency are prioritized. Its modular architecture supports standardized 1 mL, 3 mL, 6 mL, and 12 mL SPE cartridges, enabling compatibility with silica-based, reversed-phase (C18, C8), ion-exchange (SCX, SAX), and mixed-mode sorbents widely adopted in regulated workflows.

Key Features

• Twelve independently controllable flow paths, each equipped with a high-precision imported needle valve permitting fine-tuned flow regulation between 0.1 and 30 mL/min—critical for optimizing retention, washing efficiency, and elution recovery.
• Spiral-disk support plate system with infinite vertical adjustability, allowing secure positioning of collection tubes ranging from 12 mm microcentrifuge tubes to 30 mm wide-mouth vials without adapter kits.
• Monolithic borosilicate glass vacuum chamber and solvent reservoirs—chemically resistant to common organic solvents (e.g., methanol, acetonitrile, dichloromethane, ethyl acetate) and aqueous acidic/basic media—ensuring long-term dimensional stability and minimal extractable interference.
• Dual-seal anti-cross-contamination design: isolated vacuum channels prevent inter-cartridge vapor migration; integrated anti-fogging baffles suppress solvent misting during high-flow elution, preserving sample integrity and operator safety.
• Integrated analog vacuum gauge with 0–0.1 MPa range provides real-time visual feedback of system pressure—essential for monitoring column dry-down, verifying seal integrity, and troubleshooting flow inconsistencies.
• Optional accessories include large-capacity fraction collectors (up to 500 mL), nitrogen-assisted evaporation modules, and vacuum-compatible drying chambers—enabling seamless transition from SPE to concentration and reconstitution steps.

Sample Compatibility & Compliance

The SPE-12 accommodates standard 12-position SPE cartridge formats compliant with ASTM D7963-15 (Standard Practice for Solid-Phase Extraction of Organic Compounds from Water), ISO 17025-accredited laboratory requirements, and EPA Method 500/600-series protocols. Its all-glass wetted path eliminates metal leaching risks—particularly advantageous for trace-level analysis of metals, pharmaceuticals, or endocrine disruptors in environmental water or biological fluids. The system supports GLP-compliant documentation when used in conjunction with validated SOPs and manual logbook entries; while not inherently 21 CFR Part 11 compliant (as a non-software-controlled device), it integrates seamlessly into audit-trail-enabled LIMS environments via operator-recorded metadata (e.g., lot numbers, solvent batches, vacuum duration).

Software & Data Management

As a manually operated platform, the SPE-12 does not incorporate embedded firmware or digital interfaces. All operational parameters—including flow rates, solvent sequences, vacuum hold times, and cartridge conditioning steps—are documented externally per institutional SOPs. This design aligns with laboratories maintaining paper-based or hybrid electronic record systems (e.g., ELN-integrated workflows). For users requiring traceability, Hanuo provides a companion Excel-based SPE protocol template supporting batch-level tracking of cartridge lots, analyst IDs, and QC check points—compatible with internal quality review cycles and external accreditation audits (e.g., CAP, UKAS, CNAS).

Applications

The SPE-12 serves as a foundational tool across regulated and research-driven domains: environmental labs use it for pre-concentration of pesticides, PAHs, and PFAS from surface water per EPA 525.3; clinical chemistry labs apply it to isolate cortisol, vitamin D metabolites, or opioids from serum/plasma prior to LC-MS/MS; food safety laboratories employ it for mycotoxin cleanup in grain extracts (AOAC 2007.01); forensic toxicology units rely on its reproducibility for benzodiazepine screening in urine; and pharmaceutical QC labs utilize it for residual solvent removal in API intermediates per ICH Q3C guidelines.

FAQ

Is the SPE-12 compatible with polypropylene or PTFE-lined SPE cartridges?
Yes—the glass manifold and polymer tray exert no chemical incompatibility with standard commercial cartridges, including those with polypropylene housings or fluoropolymer frits.
Can the vacuum chamber be sterilized for microbiological applications?
Borosilicate glass components may be autoclaved at 121 °C for 20 minutes; however, the polymer support plate and valves are not autoclavable—disinfection via 70% ethanol wipe-down is recommended.
What vacuum pump specifications are recommended for optimal performance?
A two-stage oil-lubricated rotary vane pump delivering ≥30 L/min free air displacement and ultimate vacuum ≤0.01 MPa is advised to maintain stable –0.08 MPa operation across all 12 channels simultaneously.
Does Hanuo provide method transfer support for validated SPE protocols?
Yes—application engineers offer complimentary protocol adaptation guidance, including flow optimization curves, breakthrough volume assessments, and recovery spike studies for user-defined analyte/sorbent combinations.
Are replacement valves and glass parts available as spare components?
All consumable and wear-prone components—including needle valves, O-rings, glass reservoirs, and support disks—are stocked and supplied with full part numbers for direct reorder through authorized distributors.