Hanuo HWS-150 Temperature and Humidity Controlled Incubator
| Brand | Hanuo |
|---|---|
| Origin | Shanghai, China |
| Model | HWS-150 |
| Capacity | 150 L |
| Temperature Range (with humidity active) | 0–50 °C |
| Temperature Uniformity | ±0.5 °C |
| Humidity Range | 30–95 %RH |
| Humidity Control Accuracy | ±2 %RH |
| Construction | Stainless steel interior chamber, triple-pane insulated glass door with mechanical lock |
Overview
The Hanuo HWS-150 is a precision-engineered temperature and humidity controlled incubator designed for reproducible environmental simulation in life science laboratories. It operates on a dual-loop control architecture—separate PID-regulated systems for thermal and hygral management—ensuring stable setpoint maintenance under dynamic load conditions. The unit employs resistive heating elements combined with refrigeration-based dehumidification and ultrasonic humidification to achieve continuous, non-condensing moisture delivery across the full operational range. Its 150 L working volume is optimized for medium-throughput cell culture, microbial growth studies, BOD incubation, seed germination assays, and stability testing of pharmaceutical intermediates. The chamber’s stainless steel construction meets ISO 14644-1 Class 8 cleanroom compatibility requirements for non-sterile but controlled environments, while its triple-pane insulated glass door minimizes thermal bridging and supports real-time visual monitoring without compromising parameter stability.
Key Features
- Independent dual PID control for temperature (0–50 °C) and relative humidity (30–95 %RH), enabling simultaneous, decoupled regulation
- ±0.5 °C temperature uniformity across the entire chamber volume (per ASTM E1757-22 test protocol)
- ±2 %RH humidity accuracy verified at 25 °C/60 %RH reference point using NIST-traceable hygrometer calibration
- Stainless steel interior (AISI 304, 1.2 mm thickness) with seamless welded corners and radiused edges for easy decontamination
- Triple-pane low-emissivity glass door with integrated mechanical locking mechanism for secure access control
- Real-time digital display with intuitive menu navigation and password-protected parameter modification
- Over-temperature and over-humidity cut-off protection with audible/visual alarm outputs compliant with IEC 61000-6-2 EMC immunity standards
Sample Compatibility & Compliance
The HWS-150 accommodates standard laboratory vessels including Petri dishes (up to 150 mm diameter), multi-well plates (6–96-well), Erlenmeyer flasks (up to 2 L), and tissue culture roller bottles. Its chamber geometry supports stacked shelving (three adjustable stainless steel trays included) with ≥60 mm vertical clearance between levels for unimpeded air and vapor circulation. The unit conforms to key regulatory frameworks applicable to GLP and GMP-aligned workflows: it supports audit-ready operation per FDA 21 CFR Part 11 when paired with optional data logging software (see Software & Data Management), meets ISO 17025 traceability requirements for environmental monitoring equipment, and satisfies EN 61000-3-2 harmonic current emission limits. All materials in contact with the chamber atmosphere are RoHS-compliant and non-outgassing under sustained operation at 40 °C/80 %RH.
Software & Data Management
An optional USB-connected data logger (HWS-DL150) enables continuous recording of temperature and humidity values at user-selectable intervals (1 min to 24 h). Exported CSV files include timestamp, sensor ID, raw readings, and status flags (e.g., “alarm active”, “door open”). When deployed with Hanuo’s LabLink™ v3.2 software suite (Windows 10/11 compatible), the system supports electronic signature capture, role-based user permissions, and automated report generation aligned with ISO/IEC 17025 clause 7.7 documentation requirements. Audit trails retain all configuration changes, calibration events, and alarm history for ≥36 months—fully compliant with ALCOA+ data integrity principles. Remote monitoring via Ethernet interface (optional module) permits integration into centralized facility management systems adhering to BACnet MS/TP or Modbus TCP protocols.
Applications
- Biochemical oxygen demand (BOD5) testing per APHA Standard Methods 5210B and ISO 5815-1
- Microbial enumeration and viability assessment in food safety, water quality, and clinical microbiology labs
- Plant tissue culture and seed germination studies requiring precise hygrothermal cycling
- Stability testing of excipients and formulated APIs under ICH Q1A(R2) long-term and accelerated conditions
- Environmental stress simulation for ecotoxicology and aquatic toxicology assays (e.g., OECD 201, 210)
- Quality control of probiotic cultures, yeast propagation, and fungal sporulation protocols
FAQ
What is the minimum operating temperature when humidity is enabled?
The unit maintains humidity control down to 0 °C; however, condensation risk increases below 5 °C at >80 %RH. For sub-ambient applications, consult the optional low-temperature upgrade kit.
Is the chamber suitable for CO₂-enriched environments?
No—the HWS-150 is not equipped with gas-tight seals or CO₂ sensors. For CO₂ incubation, refer to Hanuo’s CCI series.
How often does the unit require recalibration?
Annual verification against NIST-traceable references is recommended; internal sensor drift remains within ±0.3 °C and ±1.5 %RH over 12 months under typical lab conditions.
Can the incubator be validated per IQ/OQ protocols?
Yes—Hanuo provides IQ/OQ documentation templates and factory-issued as-built drawings compliant with Annex 15 and EU GMP guidelines.
What power supply specifications are required?
220 V AC ±10 %, 50 Hz, single-phase, with dedicated 16 A circuit breaker and grounded outlet (IEC 60320 C14 inlet).
