KnowItAll Microplastics Automated Identification Software

Overview

KnowItAll Microplastics Automated Identification Software is a purpose-built spectral data analysis module developed by Wiley for high-throughput identification of microplastic particles using Raman spectroscopy. Engineered for laboratories engaged in environmental monitoring, water quality assessment, and regulatory compliance testing, this software implements chemometric pattern-matching algorithms to automate the interpretation of Raman spectra against curated, peer-reviewed spectral libraries. Unlike generic spectral search tools, the module integrates domain-specific preprocessing—such as fluorescence background subtraction, cosmic ray spike removal, and baseline correction—optimized for the low-signal, heterogeneous nature of microplastic spectra collected via confocal or micro-Raman instrumentation. It operates on the fundamental principle that microplastics (particles <5 mm) exhibit characteristic vibrational modes in the Raman fingerprint region (400–1800 cm⁻¹), enabling polymer-type classification (e.g., polyethylene, polypropylene, PET, PS, PVC) with high specificity when coupled with high-resolution spectral reference data.

Key Features

• Fully automated batch processing of Raman spectral files (e.g., .spa, .spc, .csv) from microscope-coupled Raman systems
• Integrated workflow: spectral import → pre-processing → library matching → confidence-weighted ranking → PDF report generation
• Proprietary match scoring algorithm incorporating spectral correlation, peak intensity consistency, and shape fidelity metrics
• Pre-loaded, validated Raman spectral library containing >1,200 reference spectra of virgin and environmentally aged microplastic polymers, additives, and common contaminants (e.g., cellulose, silicates)
• Customizable match thresholds and reporting templates compliant with ISO 24001:2023 and ASTM D8332-22 guidelines for microplastic identification
• Support for multi-point mapping datasets: auto-aggregates results per particle location, enabling spatial distribution analysis within filter mounts or sediment sections
• Export-ready outputs include annotated spectra overlays, polymer assignment tables, and statistical summaries (e.g., polymer type frequency, size-class distribution if paired with image metadata)

Sample Compatibility & Compliance

The software accepts spectral data acquired from any Raman spectrometer compatible with standard file formats (including Horiba LabSpec, Renishaw WiRE, Thermo Fisher OMNIC, and Bruker OPUS exports). It is validated for use with filter-based sample preparation methods per EPA Method 1613B adaptations and EU JRC Reference Protocol for Microplastics in Water. All spectral matches are traceable to NIST-traceable reference materials where available. The software architecture supports audit trails, user authentication, and electronic signature capabilities—enabling alignment with GLP (Good Laboratory Practice) and FDA 21 CFR Part 11 requirements when deployed in regulated environments. No hardware modifications or proprietary acquisition drivers are required; integration occurs at the post-acquisition data layer.

Software & Data Management

KnowItAll Microplastics Automation runs natively on Windows 10/11 (64-bit) and requires no internet connection during spectral matching—ensuring data sovereignty for sensitive environmental or forensic datasets. Spectral libraries are stored locally and version-controlled; users may extend the database with in-house reference spectra following ISO/IEC 17025-compliant curation protocols. The software logs all processing steps—including parameter settings, library versions, and operator IDs—in a tamper-evident audit log. Batch reports are generated in PDF/A-2u format for long-term archival integrity. Optional integration with LIMS platforms (e.g., Thermo SampleManager, LabVantage) is supported via RESTful API for automated result ingestion into enterprise data workflows.

Applications

• High-volume screening of wastewater effluent, drinking water, and marine sediment samples for regulatory reporting (e.g., EU Directive 2020/2184, US EPA Draft Method 1690)
• Research-grade quantification of polymer composition shifts across environmental aging gradients (UV exposure, biofouling, mechanical abrasion)
• Forensic microplastic sourcing in ecological impact assessments and source-tracking studies
• Validation of filtration efficiency in membrane-based water treatment systems
• Cross-platform method transfer between Raman instruments in multi-lab collaborative studies (e.g., GEOTRACES, GESAMP)
• Teaching laboratories requiring standardized, reproducible spectral interpretation workflows for undergraduate and graduate coursework

FAQ

Does the software require a subscription or annual license renewal?
No. The KnowItAll Microplastics Automation Module is licensed as a perpetual, node-locked software license with optional maintenance plans covering library updates and technical support.

Can I add my own reference spectra to the database?
Yes. Users may curate custom spectral collections using the built-in spectral editor and validation wizard, with metadata tagging for polymer grade, manufacturing process, and environmental conditioning history.

Is spectral calibration verification included?
Yes. The software includes automated wavelength and intensity calibration checks using internal reference peaks (e.g., silicon 520 cm⁻¹) and provides pass/fail flags aligned with ISO 17025 clause 5.9.2.

What level of technical support is provided post-purchase?
Wiley offers 24-month complimentary email and remote desktop support, plus access to application notes, webinar archives, and an online knowledge base with troubleshooting guides specific to microplastic spectral artifacts.