ROCKER Lafil 300 BioDolphin Portable Vacuum Liquid Aspiration System

Overview

The ROCKER Lafil 300 BioDolphin Portable Vacuum Liquid Aspiration System is an integrated, benchtop-scale vacuum aspiration solution engineered for safe, contamination-free handling of biological and chemical waste liquids in cell culture, microbiology, molecular biology, and general laboratory workflows. Unlike conventional vacuum setups requiring separate pumps, traps, and filtration assemblies, the Lafil 300 integrates a sealed oil-free piston vacuum pump, ergonomic handheld controller, and modular bottle-and-filter assembly into a single compact unit. Its operation relies on negative pressure generation via a dry-running piston mechanism—eliminating lubricant carryover, hydrocarbon contamination, or maintenance-related downtime. With an ultimate vacuum level of 120 mbar (equivalent to –680 mmHg) and a maximum volumetric flow rate of 20 L/min, the system delivers sufficient suction force for rapid aspiration of culture media, supernatants, wash buffers, and other low-viscosity aqueous solutions while maintaining stable performance across extended use cycles.

Key Features

• Oil-free piston vacuum pump: Eliminates risk of oil vapor contamination in sensitive biological applications; requires no lubrication, routine oil changes, or scheduled maintenance.
• Dual anti-overflow protection: Combines a mechanical float valve inside the PES waste bottle with a hydrophobic 0.2 µm PTFE disc filter positioned between the bottle and pump chamber—preventing liquid ingress into the vacuum source under overfill or sudden pressure-drop conditions.
• Compact industrial design: Occupies only 33.5 × 23 × 29.5 cm of bench space—approximately 50% less footprint than traditional pump + trap + bottle configurations—optimized for crowded biosafety cabinets and laminar flow hoods.
• Rail-style bottle retention platform: Features an integrated ABS enclosure with raised sidewalls that physically secure the 1200 mL PES waste bottle, minimizing accidental tipping during active aspiration or transport.
• Ergonomic handheld controller with lock function: Designed for prolonged one-handed operation; includes a tactile toggle switch to engage continuous aspiration without sustained finger pressure.
• Single-hand tip ejection mechanism: Utilizes an octopus-style (eight-claw) POM tip adapter enabling rapid, tool-free removal of pipette tips or tubing connectors using thumb pressure alone.
• Quick-connect modular accessories: All interface components—including stainless steel (SUS304) and POM adapters—attach via standardized push-fit couplings for rapid reconfiguration between applications.
• Autoclavable components: Waste bottles, tip adapters, and all fluid-path POM/SUS304 parts are rated for repeated steam sterilization at 121°C, 15 psi for 20 minutes—ensuring compliance with GLP and GMP environmental control requirements.

Sample Compatibility & Compliance

The Lafil 300 is validated for aspiration of non-corrosive, non-explosive aqueous-based liquids including cell culture supernatants, PBS washes, enzyme digestion mixtures, and spent growth media. It is not intended for use with organic solvents, strong acids/bases, or viscous suspensions exceeding 100 cP. The system conforms to EN 61000-6-3 (EMC Emission), EN 61000-6-2 (EMC Immunity), and EN 61010-1:2010 (Safety Requirements for Electrical Equipment for Measurement, Control, and Laboratory Use). CE marking confirms compliance with EU Machinery Directive 2006/42/EC and Low Voltage Directive 2014/35/EU. All wetted materials—including PES bottles, PTFE filters, and POM/SUS304 adapters—meet USP Class VI biocompatibility standards and are free of leachable plasticizers or heavy metals.

Software & Data Management

The Lafil 300 operates as a standalone electromechanical device with no embedded firmware, touchscreen interface, or digital connectivity. This architecture ensures operational reliability, eliminates cybersecurity vulnerabilities, and supports audit-ready manual documentation practices required under FDA 21 CFR Part 11–aligned quality systems. Users maintain full traceability through standard lab notebook entries documenting date, operator ID, application type, bottle lot number, and sterilization cycle logs. Optional accessories—including serialized bottle labels and calibrated flow verification kits—are available to support ISO/IEC 17025-compliant calibration protocols.

Applications

• Aspiration of spent culture media during subculturing of adherent and suspension mammalian cell lines
• Removal of supernatant in centrifugation workflows prior to pellet resuspension
• Drainage of wash buffers following immunostaining or ELISA plate processing
• Decanting of clarified lysates in protein purification pipelines
• General-purpose vacuum-assisted liquid transfer in BSL-2 laboratories where sterility and containment integrity are critical

FAQ

Is the Lafil 300 suitable for use inside a biosafety cabinet?
Yes—the compact dimensions, low acoustic noise (<52 dB(A)), and absence of heat-generating components make it compatible with standard Class II A2 and B2 biosafety cabinets.
Can the PES waste bottle be reused after autoclaving?
Yes—each 1200 mL PES bottle is rated for ≥50 autoclave cycles at 121°C without structural degradation or loss of hydrophobic integrity.
What is the service life of the 0.2 µm PTFE filter under normal usage?
Under typical cell culture workflows (≤2 L/day), the filter maintains >95% efficiency for ≥6 months; replacement is recommended upon visible saturation or measurable flow reduction.
Does the system support regulatory audit trails?
While the device itself does not generate electronic records, its mechanical simplicity, consistent performance, and compatibility with sterilization logbooks and manual SOPs facilitate full traceability in GLP, GMP, and ISO 13485 environments.
Are spare parts and consumables supplied with batch-specific certificates of conformance?
Yes—ROCKER provides CoA documentation for all PTFE filters, PES bottles, and SUS304 adapters upon request, including material composition, bioburden testing, and endotoxin limits per USP .