Beckman Coulter F0650 Fixed-Angle Rotor for Allegra 64R Benchtop Centrifuge
| Brand | Beckman Coulter |
|---|---|
| Origin | USA |
| Manufacturer Type | Authorized Distributor |
| Origin Category | Imported |
| Model | F0650 |
| Instrument Type | Benchtop Centrifuge |
| Maximum Capacity | 6 × 50 mL |
| Maximum Speed | 21,000 rpm (with Allegra 64R) |
| Maximum RCF | 41,400 × g (with Allegra 64R) |
| Rotor Type | Fixed-Angle |
| k-Factor | 795 |
| BioC Certification | Not Applicable |
Overview
The Beckman Coulter F0650 fixed-angle rotor is a precision-engineered centrifugation component designed exclusively for use with the Allegra 64R benchtop centrifuge platform. Engineered to deliver high-performance sedimentation under controlled gravitational forces, this rotor operates on the principle of fixed-angle centrifugation—where sample tubes are held at a constant angle (typically 35°–45°) relative to the axis of rotation. This geometry enables rapid pelleting of particles—including cellular organelles, viruses, nucleic acids, and subcellular fractions—by maximizing radial acceleration while minimizing run time and thermal load. The F0650 rotor supports up to six 50 mL conical tubes (e.g., polypropylene or polycarbonate), making it ideal for mid-throughput laboratory workflows in molecular biology, clinical diagnostics, and bioprocessing laboratories requiring reproducible, scalable separation.
Key Features
- Optimized fixed-angle geometry (35°) for enhanced pelleting efficiency and reduced resuspension during deceleration
- High-strength aluminum alloy construction with anodized surface finish for corrosion resistance and long-term mechanical stability
- Validated compatibility with standard 50 mL conical centrifuge tubes (including Beckman Coulter Polyallomer and Ultra-Clear variants)
- k-factor of 795 — enabling precise calculation of sedimentation time across varying sample viscosities and particle densities
- Autoclavable design (up to 121°C, 15 psi, 20 min) for sterility assurance in GMP-compliant environments
- Integrated imbalance detection interface compatible with Allegra 64R’s real-time rotor monitoring system
Sample Compatibility & Compliance
The F0650 rotor accommodates a broad range of tube materials and formats, including polypropylene (PP), polycarbonate (PC), and polyallomer (PA) tubes rated for ≥41,400 × g. It is routinely employed in protocols compliant with ISO 15189 (medical laboratories), CLSI EP05-A3 (precision evaluation), and USP (dissolution testing sample preparation). While not certified under BioC (a discontinued Beckman Coulter-specific validation program), the rotor meets all applicable IEC 61010-2-020 safety standards for centrifugal equipment and conforms to FDA 21 CFR Part 11 requirements when used in conjunction with Allegra 64R’s audit-trail-enabled software suite. Its design supports GLP/GMP documentation workflows through traceable rotor ID encoding and firmware-logged usage history.
Software & Data Management
When operated with the Allegra 64R centrifuge, the F0650 rotor benefits from integrated firmware-level control via Beckman Coulter’s CentriLog™ software. This platform provides full parameter logging—including speed, time, temperature, RCF, and cumulative rotor hours—with export capability to CSV and PDF formats. All runs are timestamped and user-authenticated, supporting 21 CFR Part 11 compliance through electronic signatures, audit trails, and role-based access controls. CentriLog™ also includes rotor life tracking, automatically calculating remaining operational cycles based on accumulated g-force exposure and historical usage data—ensuring adherence to manufacturer-recommended service intervals.
Applications
- Preparative isolation of mitochondria, lysosomes, and microsomes from tissue homogenates
- Clarification of bacterial and mammalian cell lysates prior to chromatographic analysis
- Concentration of viral vectors and exosomes for downstream transduction or characterization
- Routine pelleting of PCR products, plasmid preps, and RNA samples in molecular diagnostics labs
- Phase separation in solvent extraction workflows (e.g., phenol-chloroform purification)
- Quality control centrifugation in biopharmaceutical manufacturing (e.g., removal of aggregates from mAb formulations)
FAQ
Is the F0650 rotor compatible with centrifuges other than the Allegra 64R?
No—the F0650 rotor is mechanically and electronically keyed for exclusive use with the Beckman Coulter Allegra 64R benchtop centrifuge. Interchangeability with other models (e.g., Avanti J-Series or Optima MAX) is not supported.
What is the recommended maximum fill volume per 50 mL tube when using this rotor?
For optimal balance and safety, tubes should be filled to no more than 45 mL to prevent overflow during acceleration and ensure uniform heat dissipation.
Does the F0650 rotor require routine calibration?
Rotor calibration is not user-performed; however, periodic verification of speed accuracy and RCF output using NIST-traceable tachometers is recommended annually as part of preventive maintenance.
Can this rotor be used for ultracentrifugation applications?
No—it is classified as a high-speed benchtop rotor, not an ultracentrifuge rotor. Its maximum RCF (41,400 × g) falls below the 100,000 × g threshold defining ultracentrifugation.
How is rotor lifetime determined and monitored?
Lifetime is tracked via CentriLog™ software using cumulative g-hour exposure; Beckman Coulter specifies a service life of 15,000 hours or 10 years (whichever occurs first), subject to documented maintenance records.
