Panlab Oxylet Pro Animal Metabolic Caging System
| Brand | Panlab |
|---|---|
| Origin | Spain |
| Manufacturer Type | Authorized Distributor |
| Origin Category | Imported |
| Model | Oxylet Pro |
| Pricing | Upon Request |
Overview
The Panlab Oxylet Pro Animal Metabolic Caging System is a fully modular, research-grade platform engineered for comprehensive in vivo metabolic phenotyping in rodents. Built upon validated open-circuit indirect calorimetry principles, the system quantifies oxygen consumption (VO₂), carbon dioxide production (VCO₂), respiratory exchange ratio (RER), and energy expenditure in real time—enabling precise assessment of substrate utilization, thermogenesis, and metabolic flexibility. Designed for longitudinal studies under controlled environmental conditions, the Oxylet Pro integrates seamlessly with standardized housing protocols while maintaining strict adherence to NIH, AAALAC, and EU Directive 2010/63/EU guidelines for animal welfare and experimental reproducibility. Its architecture supports concurrent measurement of behavioral activity, food intake, and excretory output—making it suitable for obesity, diabetes, circadian rhythm, pharmacological intervention, and aging-related metabolism studies.
Key Features
- Modular configuration: Interchangeable Physiocage (for home-cage metabolic monitoring), Excretory Cage (for synchronized urine/fecal collection and metabolic gas analysis), and Treadmill Module (single-lane, motorized, with programmable incline/decline)
- Physiocage: Low-profile design with integrated load cells for continuous food/water intake measurement (±0.01 g resolution) and infrared beam arrays for fine-scale locomotor activity tracking (horizontal/vertical counts, rearing, ambulation)
- Excretory Cage: Patented dual-compartment waste separation system with temperature- and humidity-controlled collection zones; compatible with automated timed urine sampling and fecal pellet counting
- Treadmill Module: Individually adjustable electrical stimulation intensity per lane; speed range 0–150 cm/s; incline control from –25° to +25°; integrated VO₂/VCO₂ sampling ports aligned with treadmill exhaust stream
- Gas analysis subsystem: Dual-channel paramagnetic O₂ and NDIR CO₂ analyzers with <100 ms response time, temperature/pressure compensation, and automatic zero/span calibration
- Environmental control: Optional integration with climate chambers for ambient temperature (10–35°C), humidity (30–70% RH), and light-cycle programming
Sample Compatibility & Compliance
The Oxylet Pro is validated for use with mice (C57BL/6, BALB/c, db/db, ob/ob) and rats (Sprague-Dawley, Wistar, Zucker), accommodating body weights from 15 g to 500 g. All cages comply with IACUC-specified floor area and height requirements (e.g., NIH Guide Appendix A). Gas sampling lines meet ISO 8573-1 Class 2 purity standards for trace moisture and hydrocarbon contamination. Data acquisition conforms to GLP-compliant audit trail requirements when used with METABOLISM software configured for 21 CFR Part 11 compliance (electronic signatures, user access levels, immutable raw data logging). System validation documentation supports ISO/IEC 17025-aligned laboratory accreditation workflows.
Software & Data Management
METABOLISM software v5.2 serves as the central acquisition, visualization, and analytical engine. It provides synchronized multi-parameter time-series alignment (gas exchange, activity, intake, treadmill events) with sub-second timestamp resolution. Real-time monitoring includes dynamic waveform viewers, customizable dashboards, and live RER/energy expenditure heatmaps. Batch processing supports automated normalization to lean mass (via optional DEXA integration), circadian binning (ZT0–ZT24), and ANOVA-ready export to CSV, MATLAB (.mat), or HDF5 formats. Raw data files are stored in vendor-neutral HDF5 containers with embedded metadata (animal ID, protocol version, calibration logs, environmental setpoints), ensuring FAIR (Findable, Accessible, Interoperable, Reusable) data stewardship. Audit logs record all user actions—including parameter edits, calibration events, and report generation—with timestamps and operator IDs.
Applications
- Preclinical evaluation of anti-obesity therapeutics targeting brown adipose tissue activation or mitochondrial uncoupling
- Characterization of metabolic inflexibility in genetic models of insulin resistance and type 2 diabetes
- Circadian disruption studies assessing misalignment between feeding behavior and respiratory quotient rhythms
- Exercise physiology investigations into training-induced shifts in fuel preference (carbohydrate vs. lipid oxidation)
- Toxicology screening for drug-induced changes in basal metabolic rate and thermoregulatory capacity
- Microbiome–host interaction studies correlating fecal metabolite profiles with real-time energy expenditure patterns
FAQ
Can the Oxylet Pro be used for chronic studies exceeding 72 hours?
Yes—hardware components are rated for continuous operation up to 14 days; cage materials are autoclavable or ethylene oxide–sterilizable, and gas sensors include drift-compensation algorithms for extended stability.
Is METABOLISM software compatible with third-party physiological monitoring systems?
It supports TTL-triggered synchronization and TCP/IP-based data ingestion from external devices (e.g., telemetry ECG, core temperature loggers) via configurable API endpoints.
Does the system meet FDA requirements for IND-enabling toxicology studies?
When deployed under validated SOPs with full 21 CFR Part 11 configuration, the Oxylet Pro + METABOLISM platform satisfies data integrity expectations for nonclinical study reports submitted to regulatory agencies.
What maintenance is required for long-term reliability?
Bi-weekly zero/span calibration of gas analyzers, monthly inspection of airflow sensors and cage seals, and annual factory recalibration of load cells and treadmill encoders are recommended per Panlab’s Maintenance Protocol MP-OX-2023.
