Sanotac ePurify100 High-Pressure Preparative Liquid Chromatography System
| Origin | Shanghai, China |
|---|---|
| Manufacturer Type | Authorized Distributor |
| Domestic Origin | Yes |
| Model | ePurify100 |
| Application Level | Laboratory-Scale |
| Instrument Type | High-Pressure Preparative LC |
| Flow Rate Range | 0.01–100.00 mL/min |
| Flow Accuracy | ±1% |
| Flow Precision (RSD) | ≤0.5% |
| Maximum Pressure | 10 MPa |
| Wavelength Range | 190–800 nm |
| Wavelength Accuracy | ±1 nm |
| Wavelength Reproducibility | 0.2 nm |
| Baseline Noise | ±0.75×10⁻⁵ AU at 254 nm, TC = 1 s |
| Data Acquisition Rate | 10 Hz |
Overview
The Sanotac ePurify100 is a high-pressure preparative liquid chromatography (PLC) system engineered for precise, scalable isolation and purification of bioactive compounds from complex natural matrices—particularly functional food ingredients, nutraceuticals, herbal extracts, and synthetic intermediates. Operating on the principle of reversed-phase or normal-phase partition chromatography under controlled high-pressure conditions (up to 10 MPa), the system delivers reproducible separation performance across analytical-to-preparative flow regimes (0.01–100.00 mL/min). Its modular architecture integrates a dual-piston, microprocessor-controlled gradient pump, a dual-wavelength UV-Vis detector with real-time spectral monitoring, and an intelligent fraction collector—all coordinated via Sanochrom chromatography software compliant with FDA 21 CFR Part 11 requirements for electronic records and signatures. Designed for laboratory-scale process development, the ePurify100 supports method translation from analytical HPLC to milligram-to-gram scale purification without re-optimization, enabling rapid isolation of thermolabile or low-abundance actives such as polyphenols, flavonoids, carotenoids, and terpenoids.
Key Features
- High-precision dual-piston pump with cavity pressure feedback compensation, minimizing solvent compressibility effects and ensuring stable flow delivery across the full dynamic range.
- Advanced pulse-dampening algorithm based on cam-profile flow compensation, reducing baseline noise to ±0.75×10⁻⁵ AU (254 nm, 1 s time constant) for enhanced detection sensitivity and quantitative reliability.
- Dual-wavelength simultaneous detection (190–800 nm) with programmable wavelength stepping, stop-flow scanning, and real-time absorbance ratio calculation—enabling selective tracking of target analytes amid co-eluting impurities.
- Intelligent fraction collector with hybrid linear-rotary motion mechanics, supporting time-, peak-, threshold-, and slope-triggered collection modes; configurable for sequential, cyclic, or conditional fractionation.
- Modular column interface compatible with stainless-steel preparative columns (e.g., 10–50 mm ID) and medium-pressure glass columns (up to 100 mm ID), facilitating seamless transition between purification strategies.
- Sanochrom control software featuring audit-trail logging, user-level access control, electronic signature capability, and full compliance with GLP/GMP documentation standards.
Sample Compatibility & Compliance
The ePurify100 accommodates diverse sample formats—including crude plant extracts, fermented broths, hydrolyzed protein digests, and synthetic reaction mixtures—via flexible injection options (10 mL fixed-loop manual valve, optional loop autosampler). It supports both solid-phase and liquid-phase loading protocols, with optimized dwell volume management to preserve peak integrity during gradient elution. All wetted components—including 316L stainless-steel pump heads, PEEK-compatible fluidic paths, and quartz flow cells—are chemically inert toward common organic modifiers (acetonitrile, methanol, THF) and aqueous buffers (pH 2–9). The system meets ISO/IEC 17025 calibration traceability requirements and is routinely validated per ASTM E2631 (Standard Guide for Validation of Chromatographic Methods) and USP Chromatography. Full 21 CFR Part 11 compliance ensures data integrity for regulatory submissions in dietary supplement, food safety, and early-stage pharmaceutical development workflows.
Software & Data Management
Sanochrom software provides unified control of pump gradients, detector parameters, and fraction collection logic through an intuitive Windows-based graphical interface. Method files store complete instrument configuration—including flow profiles, wavelength programs, collection triggers, and delay volume corrections—enabling rapid recall and cross-system transfer. Raw chromatograms and fraction metadata are saved in vendor-neutral .csv and .cdf formats, supporting third-party integration with LIMS platforms (e.g., LabVantage, Thermo Fisher SampleManager). Audit trails record all user actions (method edits, run starts/stops, parameter changes) with timestamps and operator IDs. Electronic signatures are implemented using PKI-based certificate authentication, satisfying FDA-mandated requirements for regulated environments. Optional remote monitoring via secure HTTPS enables off-site system supervision without compromising network security.
Applications
- Purification of functional food actives: anthocyanins from berry extracts, curcuminoids from turmeric, resveratrol from Polygonum cuspidatum, and omega-3 fatty acid ethyl esters from fish oil hydrolysates.
- Isolation of natural pigments (e.g., betalains, chlorophyll derivatives) and flavor compounds (e.g., vanillin precursors, lactones) for clean-label ingredient development.
- Downstream processing of botanical drug candidates: ginsenosides, withanolides, and iridoid glycosides requiring high-resolution separation under mild conditions.
- Preparative-scale chiral resolution of racemic synthons used in nutraceutical formulation and prodrug synthesis.
- Method scouting and optimization for subsequent scale-up to pilot- or production-scale chromatography systems (e.g., Sanotac Pilot series).
FAQ
What column types are supported by the ePurify100?
The system accepts standard 1/4″ and 1/8″ stainless-steel preparative columns (up to 50 mm ID) and medium-pressure glass columns (up to 100 mm ID) via adaptable fittings and pressure-rated manifolds.
Does the system support gradient method development with more than two solvents?
Yes—the quaternary gradient pump allows programming of up to four mobile phase solvents, enabling complex multi-step elution profiles including step gradients, linear ramps, and re-equilibration segments.
Can I perform method validation per ICH Q2(R2) guidelines?
Absolutely. With documented system suitability testing (SST), retention time precision (RSD ≤ 0.5%), peak area repeatability (RSD ≤ 1.0%), and linearity verification across 0.1–100% of target concentration, the ePurify100 meets core ICH Q2(R2) criteria for preparative method validation.
Is the software compatible with Windows 11 and 64-bit architectures?
Yes—Sanochrom v4.2+ is fully certified for Windows 10 and Windows 11 (64-bit), with native USB 3.0 and RS-232 driver support.
What maintenance intervals are recommended for the UV detector lamp and flow cell?
Deuterium and tungsten lamps are rated for ≥2,000 hours; routine cleaning of the quartz flow cell with methanol/water (1:1) is advised after every 50 runs involving viscous or particulate-laden samples.
