Sanotac 0506 PEEK Biocompatible Medium-Pressure HPLC/Chromatography Pump for Protein Purification
| Brand | Sanotac |
|---|---|
| Origin | Shanghai, China |
| Manufacturer Type | Direct Manufacturer |
| Model | 0506 |
| Pressure Range | 0–2 MPa / 0–6 MPa / 0–10 MPa |
| Flow Rate Range | 0.1–10 mL/min / 0.1–50 mL/min / 0.1–100 mL/min / 1–200 mL/min |
| Wetted Materials | PEEK, Sapphire, Zirconia Ceramic |
| Compliance | Designed for GLP/GMP-aligned protein purification workflows |
Overview
The Sanotac 0506 is a precision-engineered medium-pressure reciprocating piston pump designed specifically for life science applications requiring chemical inertness, biological compatibility, and long-term operational stability. Built upon a dual-piston, pulse-dampened architecture, it delivers highly reproducible, continuous flow under constant pressure—critical for gradient elution in preparative chromatography, FPLC-based protein purification, nucleic acid isolation, and analytical-scale biomolecule separation. Unlike standard stainless-steel or PTFE-based pumps, the 0506 integrates chemically resistant wetted materials—including PEEK (polyether ether ketone) pump heads, sapphire check valves, and zirconia ceramic plungers—to ensure zero metal leaching, minimal adsorption of sensitive biomolecules, and full compatibility with aggressive solvents (e.g., 100% acetonitrile, 1 M NaOH, 6 M guanidine HCl) and aqueous buffers across pH 0–14. Its modular design supports scalable flow and pressure configurations, enabling seamless integration into both benchtop purification systems and automated multi-column platforms.
Key Features
- Chemically inert fluid path constructed entirely from PEEK, sapphire, and zirconia ceramic—eliminating metal ion contamination and preserving bioactivity of proteins, antibodies, and oligonucleotides
- Three pressure tiers (0–2 MPa, 0–6 MPa, 0–10 MPa) and four flow ranges (0.1–10, 0.1–50, 0.1–100, and 1–200 mL/min) configurable per application requirements
- High-precision stepper motor control with real-time feedback loop ensures flow accuracy ≤ ±0.5% RSD and pressure stability within ±0.05 MPa
- PEEK housing and tubing resist hydrolysis, fatigue, and solvent swelling—even under prolonged exposure to DMSO, DMF, or high-concentration chaotropic agents
- Low dead volume design (< 15 µL per check valve assembly) minimizes sample dilution and carryover between runs
- Front-panel digital interface with programmable start/stop, flow ramping, and pressure limit override; RS-232 and optional USB/Ethernet connectivity for system-level integration
Sample Compatibility & Compliance
The 0506 is validated for use with native and denatured proteins (including monoclonal antibodies, His-tagged enzymes, and membrane protein complexes), plasmid DNA, siRNA, and glycoproteins. Its biocompatible fluid path meets ISO 10993-5 cytotoxicity requirements and exhibits no detectable leachables per USP . The pump conforms to mechanical safety standards IEC 61010-1 and is engineered to support workflows compliant with FDA 21 CFR Part 11 when paired with audit-trail-enabled chromatography software. It is routinely deployed in laboratories adhering to GLP and GMP guidelines for process development, QC release testing, and early-stage bioprocess validation.
Software & Data Management
The pump operates natively with Sanotac’s ChromaControl Suite (v3.2+), which provides method-driven operation, real-time pressure/flow logging, event-triggered data export (CSV/Excel), and electronic signature support for regulated environments. When integrated into third-party platforms—including GE Cytiva ÄKTA systems, Bio-Rad NGC, or custom LabVIEW-based controllers—the 0506 responds to TTL and analog 0–10 V signals for synchronized gradient generation and fraction collection. All firmware updates are digitally signed and version-locked to maintain traceability during regulatory audits.
Applications
- Preparative ion-exchange and size-exclusion chromatography of therapeutic proteins
- Buffer exchange and desalting of purified enzymes prior to crystallization trials
- Gradient-elution purification of CRISPR-Cas9 ribonucleoprotein complexes
- Continuous-flow synthesis of peptide conjugates using immobilized reagents
- Process-scale polishing steps in downstream bioprocessing (up to 200 mL/min at ≤2 MPa)
- Method development for monoclonal antibody aggregate removal under native conditions
FAQ
What solvents are compatible with the PEEK fluid path?
All common HPLC-grade organic solvents (acetonitrile, methanol, isopropanol), aqueous acids (0.1–6 M HCl, HNO₃), bases (0.1–2 M NaOH, NH₄OH), chaotropes (guanidine HCl, urea), and detergents (Triton X-100, CHAPS) are fully compatible. Avoid concentrated sulfuric acid (>80%) and molten phenol.
Can the pump be used in sterile filtration or cell culture media delivery?
Yes—the PEEK/sapphire/zirconia construction is autoclavable (121°C, 20 min, 15 psi) and certified non-cytotoxic. However, sterilization must be performed without assembled tubing; reassembly requires aseptic technique.
Is remote monitoring supported for 21 CFR Part 11 compliance?
When operated with ChromaControl Suite and configured with network-enabled hardware, the system logs all user actions, parameter changes, and alarm events with time-stamped, immutable records—including operator ID and digital signature fields.
What maintenance intervals are recommended for extended service life?
Sapphire check valves should be inspected every 500 hours; PEEK seals replaced every 1,000 hours under continuous operation. Full recalibration is advised annually or after any pressure/flow deviation exceeding ±1% of setpoint.
Does the pump support gradient mixing via dual-channel configuration?
The 0506 itself is a single-channel pump. Gradient formation requires external low-dead-volume mixing tees or integration with a dedicated gradient module (e.g., Sanotac GM-12).
