SOPOTOP 756PC Ultraviolet-Visible Spectrophotometer
| Brand | SOPOTOP |
|---|---|
| Origin | Shanghai, China |
| Model | 756PC |
| Wavelength Range | 190–1100 nm |
| Optical System | Single-beam |
| Detector | Photodiode Array |
| Spectral Bandwidth | 2 nm |
| Wavelength Accuracy | ±1.0 nm |
| Wavelength Repeatability | 0.5 nm |
| Stray Light | ≤0.15% T |
| Display | 4.3-inch color touchscreen (480×272) |
| Software | UVSolution 2.0 |
| Data Interface | USB |
| Power Supply | 220 V ±10%, 50 Hz / 110 V ±10%, 60 Hz, 90 W |
| Net Weight | 10.5 kg |
| Dimensions (W×D×H) | 470×380×225 mm |
Overview
The SOPOTOP 756PC Ultraviolet-Visible Spectrophotometer is a single-beam scanning instrument engineered for routine quantitative and qualitative analysis in academic laboratories, quality control environments, and industrial R&D settings. It operates on the principle of Beer-Lambert law-based absorbance measurement across a broad spectral range from 190 nm to 1100 nm—encompassing deep UV, near-UV, visible, and near-infrared regions. Its Czerny-Turner monochromator design incorporates a 1200-line/mm holographic grating, enabling precise wavelength selection with 0.1 nm step resolution. The photodiode array detector ensures stable signal acquisition and high photometric reproducibility, while the 2 nm spectral bandwidth supports adequate resolution for most standard applications including nucleic acid quantification, protein concentration determination, and kinetic assays.
Key Features
- Single-beam optical architecture with automatic wavelength scanning and zero-absorbance calibration (100% T/0 A)
- 4.3-inch high-resolution color touchscreen interface supporting intuitive navigation and real-time spectral visualization
- Integrated UVSolution 2.0 software enabling advanced data acquisition, processing, and reporting capabilities
- Automated wavelength calibration and dark current correction routines compliant with internal instrument diagnostics protocols
- Support for multiple measurement modes: fixed-wavelength photometry, multi-point wavelength testing (up to 15 wavelengths), kinetics (time-scan with configurable intervals from 0.5 s to 60 s), and full-spectrum scanning (scan speeds up to 300 nm/min)
- Comprehensive spectral processing functions including smoothing, baseline correction, peak/valley detection, and 1st–4th order derivative spectroscopy
- Preconfigured nucleic acid and protein analysis modules with built-in extinction coefficients and ratio calculations (e.g., A260/A280)
Sample Compatibility & Compliance
The 756PC accommodates standard 10 mm pathlength cuvettes (quartz, glass, or plastic), supporting liquid-phase transmission measurements in accordance with ISO 6425, ASTM E275, and USP <857> guidelines for spectrophotometric instrumentation validation. While not inherently 21 CFR Part 11 compliant out-of-the-box, its UVSolution 2.0 software architecture allows integration into GLP/GMP workflows when deployed with validated system configurations—including user access controls, electronic signature support, and audit-trail enabled data logging. The instrument meets IEC 61010-1 safety standards for laboratory electrical equipment and complies with electromagnetic compatibility requirements per EN 61326-1.
Software & Data Management
UVSolution 2.0 is a Windows-based application providing full instrument control and post-acquisition analysis. It supports export of raw and processed data in plain-text (.txt) format for third-party statistical or chemometric analysis. Spectral plots can be saved as BMP files for publication or documentation. All measurement records—including quantitative results, calibration curves (up to 20 standards), kinetics datasets (up to 10,000 points), and scanned spectra—are timestamped and stored locally with user-defined naming conventions. The software maintains separate parameter libraries for curve-fitting models (zero-order, linear, quadratic, cubic), enabling traceable method development. Data integrity is reinforced through password-protected method templates and version-controlled configuration files.
Applications
- Quantitative determination of analyte concentrations using single- or multi-point calibration curves
- Enzyme kinetics monitoring via time-resolved absorbance at fixed wavelengths
- Verification of DNA/RNA purity and concentration using A260/A280 and A260/A230 ratios
- Colorimetric assay validation (e.g., Bradford, Lowry, BCA) in protein biochemistry
- Quality assurance of dyes, pigments, and pharmaceutical excipients across UV-Vis spectral windows
- Educational demonstrations of spectral absorption phenomena, bandgap estimation, and ligand-field transitions
FAQ
What is the maximum number of data points supported in a single kinetic run?
Up to 10,000 absorbance or transmittance readings can be acquired during one kinetics session, with user-selectable time intervals ranging from 0.5 seconds to 60 seconds.
Does the instrument support dual-wavelength or multi-wavelength ratio measurements?
Yes—UVSolution 2.0 enables simultaneous acquisition and calculation at up to three user-defined wavelengths, facilitating ratiometric assays such as NADH/NADPH monitoring or pH indicator analysis.
Is baseline correction automated during spectral scanning?
The software provides both manual and automated baseline correction tools, including reference spectrum subtraction and polynomial fitting (1st–4th order), with option to save/load baseline files for rapid retesting.
Can the 756PC be integrated into a LIMS environment?
While native LIMS connectivity is not embedded, the instrument exports ASCII-formatted data compatible with common LIMS import protocols; integration requires middleware configuration and validation per site-specific IT policies.
What maintenance procedures are recommended for long-term photometric stability?
Routine verification includes daily 100% T calibration using air or solvent blank, quarterly wavelength accuracy checks using holmium oxide or didymium filters, and annual lamp intensity profiling per SOPOTOP service recommendations.
