MKN DHP-25 Electric Constant Temperature Incubator

Overview

The MKN DHP-25 Electric Constant Temperature Incubator is a compact, benchtop-grade incubation system engineered for precise and stable thermal environments in life science laboratories. Designed around resistive external-sheath heating architecture and microprocessor-based PID temperature regulation, the DHP-25 maintains consistent chamber conditions without thermal overshoot or localized hot spots—critical for sensitive microbial cultures, cell line maintenance, enzyme assays, and short-term biological storage. Its 25-liter stainless-steel inner chamber (304 grade), combined with ≥50 mm thick polyurethane insulation and dual-layer tempered glass door, ensures high thermal retention and minimal disturbance during visual monitoring. The unit operates within a usable range of ambient +5°C to 65°C, with verified stability of ±0.3°C and uniformity of ±0.5°C at standard operating setpoints (e.g., 37°C), meeting baseline performance expectations for ISO 13485-aligned quality control labs and academic research facilities.

Key Features

• Microprocessor-controlled digital PID temperature regulation with dual LED display (setpoint and real-time chamber temperature)
• External sheath-type heating system eliminates internal radiant heat sources, preventing thermal shock to samples and ensuring linear ramp profiles
• Double-layer tempered glass observation door with silicone gasket seal—enables non-invasive visual inspection without compromising thermal integrity
• Stainless steel (AISI 304) interior chamber and rounded corners for easy cleaning and compliance with GLP hygiene protocols
• Independent mechanical over-temperature safety cutoff (non-resettable at >70°C) and electronic thermal limiter for redundant protection
• Benchtop footprint (W×D×H: 400 × 400 × 500 mm) optimized for space-constrained labs and laminar flow hood integration

Sample Compatibility & Compliance

The DHP-25 accommodates standard petri dishes (up to 150 mm), multi-well plates (6–96-well), culture flasks (T25/T75), and sterile sample tubes—compatible with routine bacteriology (e.g., E. coli, S. aureus), fungal propagation, and plant tissue explant incubation. It conforms to IEC 61010-1:2010 for electrical safety in laboratory equipment and supports basic traceability requirements under ISO/IEC 17025:2017 for calibration-aware workflows. While not certified for GMP manufacturing environments, its design aligns with FDA-recommended practices for equipment used in USP / microbiological testing support and CLIA-waived lab operations. No integrated humidity control or CO₂ regulation is provided; users requiring such functionality should consider dedicated humidified or tri-gas incubators.

Software & Data Management

The DHP-25 operates as a standalone analog-digital hybrid instrument with no embedded data logging or network interface. All operational parameters are configured manually via front-panel keypad. For laboratories requiring audit trails or electronic records, external validation-compatible temperature loggers (e.g., compliant with FDA 21 CFR Part 11 when paired with validated software platforms such as LogTag Analyzer or DicksonWare) may be deployed inside the chamber. The unit includes a factory calibration certificate traceable to NIM (National Institute of Metrology, China), and users are advised to perform periodic verification using an accredited Class II thermometer (e.g., Fluke 1523 with probe) per ASTM E2874–13 guidelines for incubator performance qualification.

Applications

• Maintenance of bacterial and yeast stock cultures at defined temperatures (e.g., 4°C for short-term refrigerated storage simulation, 30–37°C for active growth phases)
• Enzyme activity assays requiring strict thermal consistency across reaction timepoints
• Pre-incubation of agar plates prior to streaking or pour-plating in microbiology QC workflows
• Germination studies in agricultural research under controlled thermal regimes
• Stability testing of diagnostic reagents and biologics during early-phase development
• Supporting ISO 11133:2014-compliant culture media performance verification

FAQ

Does the DHP-25 include humidity control?

No. This model provides only dry-air thermal incubation. Humidity management requires optional external humidification systems or upgrade to a dedicated humidified incubator series.
Is the chamber volume adjustable or expandable?

No. The internal chamber is fixed at 25 L with non-modular shelving (one adjustable stainless-steel shelf included).
What is the recommended calibration frequency?

Annual calibration is advised for regulated environments; semi-annual verification is recommended for high-throughput academic or clinical labs per ISO/IEC 17025 Clause 6.5.2.
Can this incubator be used for mammalian cell culture?

It is suitable for short-term holding or non-CO₂-dependent cell lines (e.g., some insect or adherent immortalized lines), but lacks CO₂ regulation, humidity control, and HEPA filtration required for long-term mammalian monolayer culture.
Is the unit supplied with a calibration certificate?

Yes—a factory-issued certificate of conformance with temperature accuracy verification at 37°C is included, traceable to national metrological standards.