English Product Name

Overview

The Jihepu SPH-3222 Dual-Shelf High-Capacity Orbital Shaker is an engineered solution for scalable, reproducible suspension culture and fermentation processes in bioprocessing laboratories, pilot-scale bioreactor development, and industrial microbiology applications. Designed for integration into temperature-controlled environments—such as walk-in incubators or climate-stabilized rooms—the SPH-3222 operates as an air-bath orbital shaker, delivering consistent, low-shear rotational motion across two independently supported shelves. Its core mechanism employs a patented single-axis orbital drive system, eliminating mechanical backlash and minimizing vibration transmission to adjacent equipment. This architecture ensures long-term mechanical stability under continuous duty cycles, supporting uninterrupted operation for up to 500 hours per program sequence. Unlike conventional multi-motor or eccentric-drive shakers, the SPH-3222 achieves uniform amplitude distribution (Φ50 mm) across all positions on both shelves without calibration drift, making it suitable for comparative strain screening, media optimization, and high-throughput seed train expansion where inter-batch consistency is critical.

Key Features

• PID-controlled microprocessor system with LCD interface enabling precise, repeatable setting of speed (50–300 rpm) and time (0–500 h), with ±1 rpm frequency accuracy verified under load.
• Eight-segment programmable logic allows users to define sequential ramp-up, hold, and ramp-down phases—each with independent speed and duration parameters—for complex culture protocols such as fed-batch inoculation or thermal shock adaptation studies.
• Soft-start circuitry prevents abrupt acceleration, reducing liquid splashing and meniscus disruption during flask loading—critical for maintaining sterility and preventing cross-contamination in aerobic cultures.
• Dual-shelf configuration supports simultaneous processing of up to 108 × 500 mL Erlenmeyer flasks (or equivalent vessel formats), with standardized shelf dimensions (1108 × 740 mm) optimized for ISO-standard lab bench integration and ergonomic access.
• Integrated safety architecture includes independent over-speed detection (audible/visual alert), real-time motor temperature monitoring with automatic power cutoff, and galvanically isolated leakage current protection meeting IEC 61010-1 Class I requirements.
• Structural frame fabricated from high-tensile cold-rolled steel, reinforced with cast iron base plates and precision-machined chromium-alloy bearing housings; surface-treated with epoxy-polyester electrostatic coating for corrosion resistance in humid incubator environments.

Sample Compatibility & Compliance

The SPH-3222 accommodates standard laboratory vessels—including glass and polycarbonate Erlenmeyer flasks (50–1000 mL), baffled culture bottles, and custom bioreactor sleeves—without requiring adapter kits. Its orbital motion profile complies with ASTM D4064-20 for suspension homogeneity testing and aligns with USP recommendations for agitation consistency in cell culture process development. While not certified for GMP manufacturing use out-of-the-box, the device’s parameter memory, audit-ready timer logs, and non-volatile program storage support GLP-compliant documentation workflows. All electrical subsystems conform to CE marking directives (2014/30/EU EMC and 2014/35/EU LVD), and grounding integrity is validated per EN 61000-6-3 for laboratory electromagnetic compatibility.

Software & Data Management

The SPH-3222 operates via embedded firmware without external PC dependency. All operational data—including elapsed runtime, setpoint history, alarm events, and last-used parameters—are retained in non-volatile memory and recover automatically after power interruption (“power-loss recovery” function). The eight-segment program editor supports timestamped execution logging visible on the LCD display; however, raw data export requires manual transcription. For regulated environments, optional RS-232 or USB-to-serial adapters (sold separately) enable connection to LIMS or ELN systems, facilitating traceability under FDA 21 CFR Part 11 when paired with validated third-party software solutions.

Applications

• Microbial strain selection and high-density cultivation in antibiotic production, enzyme expression, and recombinant protein synthesis.
• Seed train scaling from shake flasks to benchtop bioreactors, ensuring hydrodynamic similarity via matched k_La and P/V ratios.
• Cell line development workflows involving CHO, HEK293, and insect cell suspension cultures requiring low-shear, oxygen-transfer-optimized agitation.
• Environmental microbiology studies involving soil slurry enrichment, wastewater microbial consortia propagation, and biodegradation assay standardization.
• Academic teaching labs conducting kinetics experiments, growth curve analysis, and mass transfer coefficient determination.

FAQ

Is the SPH-3222 compatible with CO₂ incubators?

Yes—its air-bath design and absence of internal heating elements allow safe operation inside CO₂ incubators with ambient temperature control only. Ensure proper ventilation clearance (≥10 cm on all sides) to prevent heat buildup.

Does it support external temperature feedback control?

No—the SPH-3222 is a non-thermostatic shaker; temperature regulation must be provided by the host environment (e.g., incubator chamber). It does not include integrated heaters, coolers, or PT100 probe inputs.

Can I run different speeds on each shelf?

No—both shelves operate synchronously at the same programmed speed. Independent dual-zone control is not supported.

What maintenance is required for long-term reliability?

Annual inspection of drive belt tension (if equipped), lubrication of main shaft bearings using ISO VG 68 mineral oil, and verification of ground continuity are recommended. No scheduled motor servicing is needed due to brushless AC induction design.

Is calibration documentation available?

Factory calibration certificates for speed accuracy (±1 rpm) and timer deviation (<±0.5%) are provided with each unit. On-site recalibration services are available through authorized Jihepu service partners in APAC regions.