Hujing HD-650 Horizontal Laminar Flow Clean Bench

Overview

The Hujing HD-650 Horizontal Laminar Flow Clean Bench is an ISO Class 5-certified local exhaust ventilation unit engineered for critical aseptic operations in microbiological laboratories, pharmaceutical QC environments, cell culture facilities, and academic research settings. It operates on the principle of horizontal laminar airflow—drawing ambient air through a prefilter, conditioning it via a high-efficiency particulate air (HEPA) filter rated H14 (≥99.995% efficiency at 0.3 µm), and delivering uniform, particle-free airflow across the work surface at controlled velocities between 0.25 and 0.45 m/s. Unlike vertical flow hoods, the horizontal configuration directs filtered air parallel to the work surface—from back to front—minimizing turbulence and cross-contamination risk during open-vessel manipulations such as media pouring, plate streaking, or sterile instrument handling. Its compact footprint and tabletop mounting design make it suitable for space-constrained labs where integration with existing benchtop infrastructure is required without structural modification.

Key Features

• Structural integrity: Fully welded cold-rolled steel chassis with electrostatic epoxy powder coating for corrosion resistance and ease of decontamination.
• Operator-centric ergonomics: Semi-enclosed tempered glass sash with optimized height and tilt angle to maintain laminar integrity while enabling unobstructed visual access and hand entry.
• Dual-speed fan control: Remote infrared switch enables real-time adjustment between low and high airflow modes—ensuring consistent velocity compliance across varying ambient conditions and filter loading states.
• Integrated lighting system: 5 W daylight-balanced fluorescent lamp provides ≥300 lx illumination across the full work area; independently controlled 10 W UV-C (254 nm) lamp supports periodic surface decontamination in accordance with standard microbiological safety protocols.
• Vibration-damped base: Mechanical isolation design limits transmitted vibration to ≤5 µm peak-to-peak in all three orthogonal axes—critical for applications involving sensitive optical alignment or micro-manipulation.

Sample Compatibility & Compliance

The HD-650 is validated for use with non-hazardous biological agents requiring Biosafety Level 1 (BSL-1) containment per CDC/NIH guidelines. It is not designed for toxic, volatile, or radioactive materials—nor for protection against airborne pathogens requiring BSL-2 or higher. The unit complies with ISO 14644-1:2015 for cleanroom classification, and its performance verification aligns with ISO 14644-3:2019 for testing methods—including particle counting (≥0.5 µm), airflow uniformity mapping, and microbial challenge testing (≤0.5 CFU/plate·hour using Φ90 mm settle plates). Documentation supports audit readiness for GLP and GMP environments, including traceable calibration records for airflow velocity and illumination intensity.

Software & Data Management

As a standalone mechanical system, the HD-650 does not incorporate embedded software or digital data logging. However, its dual-speed fan controller includes non-volatile memory to retain last-used mode setting after power cycling. For regulated environments, users may integrate external environmental monitoring systems (e.g., Vaisala, Omega) via analog 4–20 mA or RS-485 interfaces to log airflow velocity, UV exposure duration, and operational uptime—supporting 21 CFR Part 11-compliant electronic records when paired with validated acquisition platforms.

Applications

• Aseptic preparation of microbial culture media and agar plates.
• Inoculation and subculturing of bacterial, fungal, and yeast isolates under BSL-1 conditions.
• Handling of primary cell lines and non-transformed mammalian cells during routine passaging.
• Assembly and inspection of sterile medical device components prior to packaging.
• Quality control testing of pharmaceutical excipients and non-sterile finished dosage forms.
• Teaching laboratory demonstrations requiring reproducible laminar flow conditions for student-based microbiology exercises.

FAQ

Is the HD-650 certified to ISO Class 5 or US FED STD 209E Class 100?
Yes—the unit is tested and verified to meet ISO 14644-1:2015 Class 5 (formerly FED STD 209E Class 100) for particles ≥0.5 µm, with particle concentration ≤3,520/m³.
Can this clean bench be used for handling antibiotics or cytotoxic compounds?
No. The HD-650 provides product protection only—not personnel or environmental protection. Such applications require a Class II biological safety cabinet with inward inflow and exhausted airflow.
What maintenance intervals are recommended for the HEPA filter?
Filter service life depends on ambient air quality and usage frequency; typical replacement interval is 12–24 months. Pre-filter cleaning every 2 weeks is advised to extend HEPA longevity.
Does the unit include validation documentation for GxP environments?
Factory test reports—including airflow velocity profiles, particle count data, and UV irradiance measurements—are provided. IQ/OQ protocols can be supplied upon request for site-specific qualification.
Is electrical certification available for North American or EU markets?
Standard configuration meets CCC and CE marking requirements. UL/cUL or UKCA certification is available under custom order with additional lead time and documentation review.