BenAng UP-15UF Endotoxin-Free Ultra Pure Water System
| Brand | BenAng |
|---|---|
| Origin | Shanghai, China |
| Model | UP-15UF |
| Production Type | Domestic |
| Output Flow Rate | 15 L/h |
| Resistivity | 18.2 MΩ·cm @ 25 °C |
| Endotoxin Level | < 0.001 EU/mL |
| Microbial Count | < 1 CFU/mL |
| Particles (>0.22 µm) | < 1 /mL |
| TOC | < 10 ppb |
| System Configuration | PF + AC + RO + DI + UV (254/185 nm) + UF (5 kDa MWCO) + DMF + 0.22 µm Final Filter |
| Compliance | ASTM D1193 Type I, NCCLS CLSI EP22-A, GB/T 6682–2008 Grade I, GB/T 11446.1–1997 EL-1 |
Overview
The BenAng UP-15UF Endotoxin-Free Ultra Pure Water System is an integrated laboratory water purification platform engineered for applications demanding ultralow endotoxin, bacterial, and organic contamination—particularly in biopharmaceutical development, cell culture, monoclonal antibody production, and molecular diagnostics. It employs a multi-stage purification architecture combining pre-filtration, dual-stage carbon adsorption, high-rejection reverse osmosis (RO), mixed-bed deionization (DI), dual-wavelength UV photooxidation (254 nm for microbial inactivation; 185 nm for TOC reduction), ultrafiltration (UF) with 5,000 Da molecular weight cutoff (MWCO) for pyrogen removal, and final 0.22 µm sterile filtration. The system utilizes NASA-derived RO membrane technology with >99% salt rejection and >99.5% microbial removal efficiency, ensuring consistent delivery of Type I ultrapure water per ASTM D1193, compliant with ISO 3696 Class 1, CLSI EP22-A, and Chinese national standards GB/T 6682–2008 and GB/T 11446.1–1997 EL-1.
Key Features
- Endotoxin-free output (< 0.001 EU/mL) achieved via dedicated 5 kDa ultrafiltration module and terminal 0.22 µm sterilizing filter from Sartorius (Germany)
- Dual-wavelength UV system (254 nm + 185 nm) for simultaneous microbial inactivation and total organic carbon (TOC) photolysis, maintaining TOC < 10 ppb
- Automated RO membrane antiscalant rinse cycle triggered by pressure differential and time-based scheduling to extend membrane life and ensure long-term flux stability
- Intelligent control logic with real-time monitoring of inlet pressure, RO permeate conductivity, UP resistivity, UV lamp status, and tank level—enabling full unattended 24-hour operation
- Integrated recirculation loop with continuous ultrafiltration and UV exposure to maintain reservoir water at specification-grade purity between dispenses
- Backlit LCD resistivity monitor with temperature compensation (25 °C), displaying real-time UP water quality and historical trend logging capability
- Robust mechanical architecture featuring Kflow (USA) pumps and housings, Dow (USA) and FCS (USA) RO membranes, Rohm & Haas (USA) nuclear-grade ion exchange resins, and Pall (USA) UF cartridges
Sample Compatibility & Compliance
The UP-15UF is validated for feed water meeting municipal tap specifications: TDS ≤ 200 ppm, temperature 5–40 °C, and inlet pressure 1.0–3.5 kg/cm² (0.1–0.35 MPa). Its output meets or exceeds the most stringent regulatory benchmarks for critical life science workflows—including USP , FDA 21 CFR Part 11 data integrity requirements (when paired with optional audit-trail-enabled software), ISO/IEC 17025 traceability protocols, and GMP/GLP environmental monitoring validation frameworks. All wetted components comply with USP Class VI biocompatibility standards; tubing and housings are constructed from pharmaceutical-grade polypropylene and PVDF to prevent leachables. The system supports IQ/OQ documentation packages for laboratory qualification under cGMP environments.
Software & Data Management
While the base UP-15UF operates via embedded microcontroller logic with local LCD interface, optional Sciencetool (USA) digital control modules enable Ethernet/Wi-Fi connectivity, remote status monitoring, and automated log export (CSV/Excel) of resistivity, TOC, UV intensity, and alarm history. All stored data include timestamp, operator ID (if configured), and environmental context (e.g., ambient temperature, inlet pressure deviation). Audit trail functionality satisfies FDA 21 CFR Part 11 requirements for electronic records and signatures when deployed with role-based access control and encrypted storage. Calibration events, filter replacement logs, and service alerts are time-stamped and non-erasable.
Applications
- HPLC, UHPLC, and LC-MS mobile phase preparation and instrument rinsing
- Cell culture media formulation, transfection reagent dilution, and stem cell expansion protocols
- ELISA, Western blotting, and qPCR reagent preparation requiring endotoxin-sensitive assays
- Protein crystallization, affinity chromatography, and SEC-MALS buffer preparation
- ICP-MS, GF-AAS, and ICP-OES standard dilution and blank matrix generation
- Semiconductor wafer cleaning, photomask rinsing, and thin-film deposition precursor dilution
- Reference material certification and metrological traceability workflows per ISO/IEC 17025
FAQ
What is the primary mechanism for endotoxin removal in the UP-15UF system?
Endotoxin removal is achieved primarily through the 5,000 Da molecular weight cutoff (MWCO) ultrafiltration (UF) cartridge—designed to retain lipopolysaccharide (LPS) aggregates—and secondarily by the sterile 0.22 µm final filter from Sartorius.
Does the system support continuous recirculation to maintain reservoir water quality?
Yes—the UP-15UF incorporates an internal closed-loop recirculation path that continuously passes stored ultrapure water through the UF and dual-wavelength UV modules to suppress microbial regrowth and minimize TOC accumulation.
Is the resistivity measurement temperature-compensated?
Yes—the built-in resistivity sensor applies automatic temperature compensation to report values normalized to 25 °C, conforming to ASTM D1125 and ISO 7888 standards.
Can the UP-15UF be integrated into a centralized lab water distribution network?
It is designed as a point-of-use system; however, with optional booster pump and pressure-regulated dispensing arm, it may serve as a dedicated feed source for low-flow analytical instruments without compromising purity.
What maintenance intervals are recommended for consumables?
Pre-filters: 12–24 months depending on feed water quality | RO membrane: 2–3 years | UF cartridge: 12–18 months | UP polishing column: 6–12 months (monitor resistivity decay and TOC rise) | Final 0.22 µm filter: replaced per batch or after 1,000 L, whichever occurs first.
