Yoyilab SPX-250 Biochemical Incubator

Overview

The Yoyilab SPX-250 Biochemical Incubator is a precision-engineered, forced-air convection incubator designed for stable, reproducible temperature control in microbiological, environmental, and life science applications. Built upon a dual-mode thermoregulation architecture—integrating high-efficiency compressor-based refrigeration with PTC heating elements—the SPX-250 delivers bidirectional thermal control across a wide operational range of 0–65 °C. Its microprocessor-driven PID temperature regulation system ensures minimal thermal drift and high repeatability under variable load conditions, making it suitable for critical protocols including biochemical oxygen demand (BOD) testing per ASTM D5210 and ISO 5815, microbial culture maintenance (e.g., E. coli, Bacillus spp., Aspergillus niger), and long-term stability studies in pharmaceutical and food safety laboratories. The chamber’s structural integrity, combined with rigorous thermal validation at multiple spatial points, supports compliance with GLP-aligned experimental workflows and routine QC/QA requirements.

Key Features

• Robust cold-rolled steel exterior with electrostatic epoxy powder coating for corrosion resistance and mechanical durability
• Polished stainless-steel interior chamber with seamless, radius-cornered construction for easy decontamination and reduced microbial retention
• Dual-pane insulated door with low-emissivity glass window, minimizing heat loss during observation without compromising chamber stability
• Adjustable stainless-steel shelving system (standard configuration: 2–3 trays) supporting flexible load distribution and airflow optimization
• Integrated castor wheels with independent locking feet enabling both mobility and vibration-isolated stationary operation
• 5.7-inch backlit LCD interface with menu-driven navigation—displaying real-time setpoint, actual chamber temperature, elapsed time, and timer countdown simultaneously
• Intelligent power-loss recovery function preserving user-defined parameters and active session states after unexpected shutdowns
• Dual-stage overtemperature protection: primary PID control loop plus independent hardware-based cutoff threshold (adjustable up to 70 °C)

Sample Compatibility & Compliance

The SPX-250 accommodates standard Petri dishes (90–150 mm), multi-well plates (6–96-well), culture flasks (T25–T225), and BOD bottles (300 mL) on its adjustable shelves. Its uniform airflow design—validated at 25 °C per IEC 61000-4-2—ensures ≤±1.5 °C spatial deviation across the full working volume, meeting acceptance criteria for ISO 13408-1 (aseptic processing environments) and USP (pharmaceutical stability chambers). While not certified to IEC 61010-1 Class II or UL 61010B out-of-the-box, the unit complies with CE-marking essential requirements for laboratory equipment when operated within specified ambient conditions (+5 to +30 °C, non-condensing humidity ≤80% RH). Optional RS485 communication enables integration into centralized lab monitoring systems compliant with FDA 21 CFR Part 11 audit trail requirements when paired with validated software.

Software & Data Management

The embedded controller logs temperature data at user-configurable intervals (1–60 min) with internal storage capacity for ≥30 days of continuous recording. Optional RS485 interface (add-on module) supports Modbus RTU protocol for connection to SCADA platforms or LIMS via third-party gateways. An optional embedded thermal printer provides hard-copy output of temperature profiles and alarm events—critical for ISO/IEC 17025-accredited labs requiring traceable documentation. All firmware updates are performed via USB port using signed binary packages; no cloud dependency or remote access capability is implemented, ensuring data sovereignty and network security in regulated environments.

Applications

• Biochemical Oxygen Demand (BOD₅) analysis per APHA Standard Methods 5210B and EPA Method 405.1
• Microbial enumeration and viability assays (coliforms, yeasts, molds) in environmental water, food, and clinical samples
• Long-term strain preservation at sub-optimal growth temperatures (e.g., 4 °C for short-term, 25 °C for mesophilic maintenance)
• Plant tissue culture initiation and callus proliferation under controlled thermal regimes
• Enzyme activity profiling and protein stability screening across defined temperature gradients
• QC incubation of media plates prior to sterility testing in pharmaceutical manufacturing (Annex 1-aligned workflows)

FAQ

What is the minimum ambient temperature required for reliable compressor operation?
The unit requires an ambient environment between +5 °C and +30 °C; operation below +5 °C may impair refrigerant cycle efficiency and extend cooldown times.
Can the SPX-250 be used for CO₂-sensitive cultures?
No—this model lacks CO₂ concentration control or humidification systems; it is strictly a temperature-controlled air incubator.
Is the temperature uniformity specification verified under loaded or unloaded conditions?
Per manufacturer validation protocol, uniformity is measured at 25 °C under empty-chamber conditions per ISO 16770 Annex B; loaded uniformity depends on rack configuration and sample thermal mass.
Does the incubator support programmable ramp-hold temperature profiles?
Not natively—the standard controller supports single-setpoint operation with timer-based start/stop; multi-segment programming requires the optional external programmable controller (part #SPX-PC-1500, sold separately).
What calibration documentation is provided with shipment?
Each unit ships with a factory-issued temperature verification report (NIST-traceable probe at center-point, 25 °C and 37 °C), but formal IQ/OQ documentation must be generated on-site per user SOPs.