Shanghai Youyi LRH-150CL Low-Temperature Incubator & Storage Chamber

Overview

The Shanghai Youyi LRH-150CL is a precision-engineered low-temperature incubator and controlled-environment storage chamber designed for stable, long-term sample preservation and temperature-sensitive biological or material conditioning. It operates on a dual-mode thermodynamic principle—combining vapor-compression refrigeration with intelligent PID-controlled heating—to maintain setpoints across an exceptionally wide operational range of −40 °C to +65 °C. Unlike standard ambient or refrigerated incubators, the LRH-150CL integrates cryogenic capability with precise thermal stability, enabling applications spanning cryopreservation of sera and vaccines, low-temperature microbial cultivation, accelerated stability testing per ICH Q1 guidelines, and environmental stress screening of electronic components and polymer-based materials. Its robust architecture supports uninterrupted operation in laboratory environments where reliability, repeatability, and regulatory traceability are essential.

Key Features

• Compressor-driven refrigeration system using environmentally compliant R134a/R406a blended refrigerant—meeting global ozone depletion potential (ODP = 0) and low global warming potential (GWP) standards.
• Microprocessor-based temperature control with 5.7-inch LCD touchscreen interface; real-time display of setpoint, actual chamber temperature, elapsed time, and programmable hold duration.
• High-efficiency air circulation system featuring a branded centrifugal blower and optimized duct geometry—ensuring ≤ ±0.5 °C uniformity across the full 150 L working volume under no-load conditions (per ISO 15330:2021 test protocol).
• Double-glazed, low-emissivity inner door with magnetic seal and anti-fog coating—minimizing thermal intrusion during observation without compromising internal stability.
• Electrostatically coated cold-rolled steel exterior and mirror-finish stainless-steel interior (SUS304); fully adjustable, corrosion-resistant shelving with removable trays (standard configuration: 3 shelves, up to 19 positions).
• Power-loss recovery function with non-volatile memory—automatically restoring pre-interruption parameters upon re-energization, including temperature setpoint, timer status, and alarm thresholds.
• Integrated safety protections: independent over-temperature cut-off, compressor overheat lockout, door-open alarm, and audible/visual fault indication.

Sample Compatibility & Compliance

The LRH-150CL accommodates a broad spectrum of sample formats—including serum vials (2–50 mL), culture flasks (T25–T225), Petri dishes, multiwell plates, and sealed environmental test chambers—within its 480 × 390 × 780 mm working chamber. Its design conforms to key international laboratory equipment standards: IEC 61010-1 (safety requirements for electrical equipment), ISO 13485 (for medical device-related storage validation), and ASTM E2875-22 (standard guide for qualification of environmental chambers). While not certified as a medical device under FDA 21 CFR Part 820, it supports GLP/GMP-aligned workflows when paired with optional data logging modules and audit-trail-capable software (e.g., optional RS485/USB interface with timestamped CSV export).

Software & Data Management

The base unit operates via embedded firmware supporting manual mode, timed hold, and continuous operation. Optional connectivity packages extend functionality: USB/RS485 interfaces enable integration with LabVIEW, MATLAB, or custom SCADA systems; bundled PC software provides real-time graphing, configurable alarm thresholds, and exportable .csv logs compliant with ALCOA+ principles (Attributable, Legible, Contemporaneous, Original, Accurate, Complete, Consistent, Enduring, Available). An optional embedded thermal printer (25 mm paper width) delivers hard-copy records suitable for audit submission. All firmware versions include firmware signature verification and password-protected parameter editing to meet basic 21 CFR Part 11 readiness requirements when deployed in validated environments.

Applications

• Cryostorage of biological reagents: human serum, monoclonal antibodies, lyophilized enzymes, and reference standards at −20 °C to −40 °C.
• Vaccine stability assessment per WHO TRS 999 Annex 5 and USP , including freeze-thaw cycling and real-time/accelerated shelf-life studies.
• Low-temperature microbiology: psychrophilic bacterial culture (e.g., Pseudomonas fluorescens, Colwellia psychrerythraea) and fungal strain maintenance.
• Material science testing: thermal aging of adhesives, encapsulants, and PCB substrates per IPC-TM-650 2.6.7.
• Environmental simulation for electronics: cold-soak testing of sensors, MEMS devices, and battery modules prior to functional validation.
• Pharmaceutical QC labs: intermediate storage of HPLC mobile phases, reference standards, and stability batch samples under controlled temperature regimes.

FAQ

What is the minimum achievable temperature, and how long does it take to reach −40 °C from ambient?
The LRH-150CL achieves −40 °C within approximately 120 minutes under no-load conditions at 25 °C ambient, per manufacturer test report SHYY-LRH-150CL-TP-2023-08.
Does the unit support multi-segment temperature programming?
Yes—when equipped with the optional programmable controller (part #LRH-PROG-150), users can define up to 30 segments with ramp rates, dwell times, and cycle repeats.
Is calibration documentation provided with shipment?
Each unit ships with a factory-as-tested certificate listing temperature uniformity, stability, and sensor offset values at three points (top/middle/bottom) across −40 °C, 4 °C, and 37 °C.
Can the chamber be validated for GxP use?
Yes—the LRH-150CL is IQ/OQ-ready; Shanghai Youyi provides URS templates, DQ checklists, and optional PQ protocol support through authorized third-party validation partners.
What maintenance intervals are recommended for the refrigeration system?
Compressor oil and refrigerant levels should be verified annually; condenser coil cleaning is advised every six months in dust-prone environments. Full service is recommended every 24 months by certified SHYY field engineers.