Agilent 1200 Series HPLC System with Multi-Wavelength Detector (MWD)

Overview

The Agilent 1200 Series HPLC System with Multi-Wavelength Detector (MWD) is a high-performance, modular liquid chromatography platform engineered for precision separation, quantification, and spectral characterization of small- to medium-molecular-weight compounds. Built upon Agilent’s proven quaternary pump architecture and low-dispersion flow path design, this system employs high-pressure gradient elution (up to 400 bar) and supports isocratic or multi-step gradient methods with exceptional baseline stability and retention time reproducibility. The integrated G1365B Multi-Wavelength Detector enables simultaneous acquisition of UV-Vis absorbance spectra across 190–950 nm at up to 80 Hz sampling rate, supporting peak purity assessment, spectral library matching, and method development for complex matrices. As a refurbished instrument certified by SpectraLab Scientific, each unit undergoes full functional validation—including pump linearity, detector noise/drift, autosampler precision, and column oven temperature uniformity—ensuring compliance with routine QC, pharmaceutical stability testing, and academic research requirements.

Key Features

• G1311A Quaternary Pump: Delivers precise, pulse-free solvent delivery with flow accuracy ±0.2% and flow precision RSD < 0.07% at 1 mL/min.
• G1379B Vacuum Degasser: Four-channel membrane-based degassing with real-time pressure monitoring and automatic purge cycle initiation.
• G1329A/G1367B Autosampler: Supports 100-position vial capacity, injection volume range 0.1–100 µL, and carryover < 0.01% with needle wash function.
• G1316A Column Compartment: Maintains temperature control from 4 °C to 80 °C (±0.15 °C stability), compatible with columns up to 250 mm in length.
• G1365B Multi-Wavelength Detector: Dual-beam optics with deuterium and tungsten lamps; configurable wavelength windows, photodiode array resolution ≤ 1.2 nm, and spectral bandwidth ≤ 4 nm.
• G1330B Thermostat: Provides independent temperature regulation for detector flow cell and auxiliary modules, minimizing thermal drift during extended runs.
• Refurbishment Protocol: Includes replacement of critical wear components (seals, pistons, lamp housings), recalibration against NIST-traceable standards, and full system IQ/OQ documentation per ISO/IEC 17025 guidelines.

Sample Compatibility & Compliance

The Agilent 1200 MWD system accommodates reversed-phase, normal-phase, ion-exchange, and size-exclusion chromatographic modes using standard 3.9–4.6 mm ID analytical columns. It is routinely deployed in regulated environments where data integrity and audit readiness are essential. The system supports 21 CFR Part 11-compliant software configurations (e.g., OpenLab CDS) when paired with appropriate access controls, electronic signatures, and audit trail activation. Method validation parameters—including specificity, linearity (r² ≥ 0.999), LOD/LOQ, and robustness—meet ASTM E2656 and ICH Q2(R2) expectations. All refurbished units conform to Agilent’s original equipment specifications and are verified against USP , EP 2.2.46, and ISO 17025-accredited performance criteria.

Software & Data Management

Data acquisition and processing are performed via Agilent OpenLab CDS (ChemStation Edition or modern CDS 2.x), enabling automated peak integration, spectral deconvolution, library search (e.g., Wiley UV Libraries), and report generation compliant with GLP and GMP documentation standards. Raw data files (.D directories) are stored in vendor-neutral formats supporting long-term archival and third-party reprocessing. Audit trails record all user actions—including method edits, integration parameter changes, and result approvals—with immutable timestamps and operator identification. Optional network licensing allows centralized instrument management across multi-user laboratories while maintaining individual accountability per FDA and EMA guidance.

Applications

• Pharmaceutical QC: Assay, related substances, and dissolution testing per USP monographs.
• Food & Beverage Safety: Pesticide residue screening, mycotoxin quantification, and additive profiling.
• Environmental Analysis: PAHs, PCBs, and emerging contaminants in water and soil extracts.
• Academic Research: Natural product isolation, metabolite identification, and polymer characterization.
• Biopharmaceutical Support: Small-molecule excipient analysis and stability-indicating assays for biologics formulations.

FAQ

Is this system fully validated and ready for regulated use?
Yes—each unit undergoes IQ/OQ verification per ISO/IEC 17025 and includes a Certificate of Conformance with test records covering pump performance, detector linearity, autosampler precision, and temperature stability.
What warranty coverage is provided?
A comprehensive 6-month parts-and-labor warranty is included, extendable to 12 months with optional service agreement. Coverage applies globally under Agilent-authorized refurbishment terms.
Can the system be integrated into an existing OpenLab network?
Absolutely—the refurbished 1200 MWD retains full compatibility with Agilent OpenLab CDS 2.x and legacy ChemStation platforms, including secure LDAP authentication and centralized license management.
Are consumables and spare parts available?
Yes—SpectraLab Scientific maintains inventory of genuine Agilent seals, lamps, fuses, and column hardware, with same-day shipping for North America and EU destinations.
Does the refurbishment include software licensing?
The system ships with pre-installed, activated OpenLab CDS software; perpetual licenses are included for the base configuration, with upgrade paths available through Agilent’s authorized channel.