Agilent 1220 Infinity II Used High-Performance Liquid Chromatograph

Overview

The Agilent 1220 Infinity II Used High-Performance Liquid Chromatograph is a rigorously validated, field-proven HPLC system engineered for robust routine analysis in regulated and non-regulated laboratory environments. Based on Agilent’s Infinity II platform architecture, it implements conventional high-pressure liquid chromatography (HPLC) principles with compatibility for ultra-high-performance liquid chromatography (UHPLC)-capable columns—leveraging 600 bar pressure capability to exploit sub-2 µm particle-packed stationary phases for enhanced resolution, peak capacity, and reduced analysis time. Designed for operational continuity, the system features a modular, serviceable architecture that supports long-term reliability with minimal downtime. Its mechanical and electronic subsystems—including dual-piston reciprocating pump, thermally stabilized column compartment, and high-speed variable wavelength detector—are calibrated and verified per Agilent’s factory acceptance test (FAT) protocols prior to recommissioning. This unit is particularly suited for laboratories requiring FDA 21 CFR Part 11–compliant data integrity frameworks, GLP/GMP-aligned workflows, or mobile analytical deployment where space, power, and environmental stability constraints apply.

Key Features

• 600 bar binary or quaternary solvent delivery system with low dwell volume and pulse-dampened flow for high reproducibility across gradient methods
• Thermally controlled column oven maintaining ±0.1 °C stability over 5–85 °C range, minimizing retention time drift
• Variable wavelength UV-Vis detector with 190–700 nm spectral range, 80 Hz acquisition rate, and <0.0005 AU noise specification (typ.) at 254 nm
• Integrated autosampler supporting up to 132 vials (2 mL), with needle wash station, pressure-tolerant injection valve (600 bar), and programmable partial-loop and full-loop modes
• Modular detector expansion support: compatible with Agilent 1200-series RID, FLR, ELSD, and direct coupling to Agilent 6100/6200 series single-quadrupole mass spectrometers
• Pre-commissioned configuration: each refurbished unit undergoes functional verification including leak testing, baseline noise assessment, retention time precision (RSD < 0.15%), and integration accuracy per ASTM D7217 and ISO 17025 traceable procedures

Sample Compatibility & Compliance

The Agilent 1220 Infinity II accommodates standard 4.6 mm × 150 mm HPLC columns as well as narrow-bore (2.1 mm i.d.) UHPLC columns packed with 1.7–1.8 µm particles. It supports aqueous-organic mobile phases (e.g., water/acetonitrile, water/methanol), ion-pair reagents, and volatile buffers compatible with downstream MS detection. System validation documentation includes IQ/OQ reports aligned with USP , EP 2.2.46, and ICH Q2(R2) guidelines. All firmware versions are locked to Agilent-recommended revision levels for regulatory consistency; no unauthorized patches or third-party modifications are present. The instrument meets CE marking requirements (2014/30/EU EMC Directive, 2014/35/EU LVD) and complies with RoHS 2011/65/EU material restrictions.

Software & Data Management

Two certified software environments are supported: Agilent ChemStation Rev. C.01.09 or later, and EZChrom Elite v.3.3.2 SP1 or higher. Both platforms provide audit-trail-enabled operation, electronic signature support (per 21 CFR Part 11 Annex 11), and raw data export in vendor-neutral formats (e.g., .cdf, .csv). EZChrom Elite enables centralized method management, sequence scheduling, and real-time chromatogram review across networked workstations. ChemStation offers backward compatibility with legacy Agilent 1100/1200 methods and integrates with LIMS via ODBC or Agilent OpenLab CDS interfaces. All software installations include licensed dongles or node-locked activation; no trial or unlicensed modules are deployed.

Applications

This system is routinely deployed for pharmaceutical assay and impurity profiling (ICH Q5, Q6A), food safety testing (pesticide residue screening per AOAC 2012.01), environmental contaminant quantification (EPA Method 8330B for PAHs), and quality control of excipients and active pharmaceutical ingredients (APIs). Its thermal and pressure stability make it suitable for method transfer from R&D to QC labs, while its compact footprint (<0.5 m² floor space) and low power draw (<300 W) enable integration into mobile analytical units—such as containerized field labs or vehicle-mounted environmental monitoring stations.

FAQ

Is this unit covered by Agilent’s original manufacturer warranty?
No. This is a professionally refurbished unit backed by a 6-month limited hardware warranty covering defects in materials and workmanship under normal laboratory use.
Can this system be upgraded to support GPC/SEC or chiral separations?
Yes—via installation of Agilent 1200 Series refractive index detector (RID) or chiral-specific columns with compatible mobile phase delivery; column oven temperature programming ensures optimal enantioselectivity.
Does the system include validated IQ/OQ documentation?
Yes—each unit ships with a complete, instrument-specific IQ/OQ package compliant with ISO/IEC 17025 and GxP expectations, including calibration certificates traceable to NIST standards.
What maintenance history is available for this specific unit?
Full service logs—including pump seal replacements, lamp usage hours, detector cell cleaning records, and firmware update timestamps—are provided upon request prior to purchase.
Is remote diagnostics or Agilent CrossLab support available post-purchase?
Yes—optional annual CrossLab Care contracts provide 24/7 remote troubleshooting, priority spare parts dispatch, and on-site engineer dispatch within 48 business hours in North America and EU regions.