Agilent 1220 Infinity Liquid Chromatograph (Refurbished)

Overview

The Agilent 1220 Infinity Liquid Chromatograph is a rigorously refurbished, fully validated benchtop HPLC system engineered for high-throughput routine analysis in regulated and non-regulated laboratory environments. Based on Agilent’s proven 1200 Infinity platform architecture, this system employs high-pressure liquid chromatography (HPLC) separation principles—utilizing precise solvent delivery, reproducible sample introduction, temperature-controlled column retention, and UV-Vis absorbance detection—to resolve complex mixtures with robust method transfer capability. Designed for compatibility with both conventional 3–5 µm particle columns and sub-2-µm UHPLC-grade packings (up to 400 bar), the 1220 Infinity delivers enhanced resolution, reduced analysis time, and improved peak shape without requiring full UHPLC infrastructure. Its modular design supports seamless integration into existing workflows, making it especially suitable for QC/QA labs, academic core facilities, contract research organizations (CROs), and mobile analytical units where reliability, operational uptime, and cost-efficiency are critical.

Key Features

• High-stability quaternary or binary gradient pumping system with active solvent selection and integrated degasser, delivering flow accuracy ≤ ±0.2% RSD and pressure pulsation < 0.1% RMS
• 600-bar capable autosampler with low carryover (< 0.005%) and programmable needle wash, supporting 100-position 1.5 mL vial trays and injection volumes from 1–50 µL with 0.1 µL increment precision
• Thermostatically controlled column compartment maintaining ±0.15 °C stability across 10–60 °C range, minimizing retention time drift and improving inter-day reproducibility
• Variable wavelength UV-Vis detector with dual-lamp source (D₂/W), 190–700 nm spectral range, 100 Hz data acquisition rate, and noise level ≤ ±0.75 × 10⁻⁵ AU at 254 nm
• Pre-installed Agilent Instrument Utilities software for instrument control, basic data acquisition, and diagnostic monitoring—fully compatible with Agilent OpenLab CDS and ChemStation platforms
• Factory-refurbished and performance-verified per Agilent’s certified refurbishment protocol, including leak testing, pressure calibration, baseline noise validation, and gradient accuracy verification
• Modular expandability: supports optional detectors (RID, FLR, ELSD), fraction collectors, and direct coupling to Agilent 6100 Series single-quadrupole mass spectrometers via standard LC/MS interfaces

Sample Compatibility & Compliance

The Agilent 1220 Infinity accommodates a broad spectrum of sample matrices—including pharmaceutical actives, environmental extracts, food additives, polymer degradation products, and natural product isolates—when paired with reversed-phase, normal-phase, HILIC, or ion-exchange columns. Its hardware and firmware comply with key regulatory expectations for analytical instrumentation used in GxP environments. The system supports audit trail generation, electronic signatures, and user-access controls when operated under Agilent OpenLab CDS Enterprise Edition (21 CFR Part 11 compliant). Method parameters, sequence logs, and raw data files adhere to ASTM E2524-21 and ISO/IEC 17025:2017 documentation requirements. All refurbished units undergo IQ/OQ documentation support upon request, aligning with GLP and GMP laboratory validation practices.

Software & Data Management

Out-of-the-box operation utilizes Agilent Instrument Utilities—a lightweight, Windows-based application enabling real-time pump control, autosampler programming, detector configuration, and basic chromatogram viewing. For advanced processing and compliance-ready reporting, the system integrates natively with Agilent ChemStation (Rev. B.04.03 or later) and OpenLab CDS (v2.5+). These platforms provide peak integration algorithms compliant with USP , customizable report templates, multi-user permission hierarchies, and secure database archiving. Raw data (.ch files) are stored in vendor-neutral formats compatible with third-party review tools (e.g., ACD/Labs, SimGlycan). All software installations include license keys validated for the specific instrument serial number; no dongles or network licensing dependencies are required.

Applications

• Pharmaceutical quality control: assay, related substances, dissolution testing, and stability-indicating methods per ICH Q2(R2)
• Environmental analysis: PAHs, pesticides, and endocrine disruptors in water and soil extracts (EPA Methods 8330B, 8081B)
• Food and beverage testing: preservatives (sorbates, benzoates), sweeteners (aspartame, sucralose), and mycotoxins (aflatoxins, ochratoxin A)
• Academic research: natural product purification monitoring, metabolite profiling, and synthetic reaction tracking
• Contract laboratory services: method development, validation support, and batch release testing under ISO/IEC 17025 accreditation scope

FAQ

Is this unit covered by an Agilent warranty?
Yes—each refurbished Agilent 1220 Infinity system includes a 12-month limited hardware warranty covering parts and labor, backed by Agilent-authorized service engineers.
Can I upgrade to a PDA or MS detector later?
Absolutely—the system features standardized optical and electrical interfaces compatible with Agilent 1260 Infinity II DAD and 6100 Series LC/MS systems; upgrade kits and integration support are available.
Does it meet FDA 21 CFR Part 11 requirements?
The base configuration does not include Part 11 functionality, but full compliance is achieved when deployed with Agilent OpenLab CDS Enterprise Edition and properly configured server infrastructure.
What documentation is provided with the system?
You receive a Certificate of Refurbishment, IQ/OQ execution records (optional add-on), complete operation manuals, software installation media, and a traceable calibration certificate for all critical modules.
Is remote diagnostics supported?
Yes—Agilent Remote Monitoring Services (RMS) can be enabled via secure VPN connection, allowing predictive maintenance alerts and real-time performance telemetry.