Agilent 8453 UV-Vis Spectrophotometer (Refurbished)
| Brand | Agilent Technologies |
|---|---|
| Origin | USA |
| Model | 8453 |
| Detector Type | Photodiode Array (PDA) |
| Wavelength Range | 190–1100 nm |
| Wavelength Accuracy | < ±0.2 nm |
| Spectral Bandwidth | 1 nm |
| Stray Light | ≤ 0.03% T |
| Automation Level | Automatic Wavelength Scanning |
| Instrument Classification | Imported High-Performance UV-Vis Spectrophotometer |
Overview
The Agilent 8453 UV-Vis Spectrophotometer is a high-precision, diode-array-based absorption spectrometer engineered for quantitative and qualitative analysis across pharmaceutical, environmental, food safety, and academic research laboratories. Utilizing a fixed grating monochromator coupled with a 1024-element photodiode array detector, the system acquires full UV-Vis spectra (190–1100 nm) in under one second—enabling real-time kinetic monitoring, spectral library matching, and high-throughput method development. Its optical design minimizes wavelength drift and photometric noise, delivering robust baseline stability and high reproducibility essential for GLP-compliant workflows. As a refurbished instrument, each unit undergoes comprehensive factory-level recalibration, optical alignment verification, lamp intensity profiling, and electronic diagnostics per Agilent’s original service specifications—ensuring performance equivalence to new-unit operational thresholds.
Key Features
- Photodiode array (PDA) detection architecture enabling simultaneous multi-wavelength acquisition without mechanical scanning
- Optimized optical path with quartz-coated optics and thermally stabilized cuvette compartment for < ±0.002 AU/h baseline drift
- Wavelength accuracy certified to < ±0.2 nm (verified against NIST-traceable holmium oxide and didymium filters)
- Spectral bandwidth of 1 nm (FWHM), supporting resolution-critical applications such as overlapping peak deconvolution in complex matrices
- Stray light performance ≤ 0.03% T at 220 nm (NaI) and 340 nm (NaNO₂), meeting ASTM E275 and ISO 9001 spectral purity requirements
- Integrated tungsten-halogen and deuterium dual-source lamp system with automatic source switching and lifetime monitoring
- USB and RS-232 interfaces compatible with legacy and modern laboratory data systems
Sample Compatibility & Compliance
The Agilent 8453 supports standard 10-mm pathlength quartz, glass, or plastic cuvettes—including matched pairs for ratio-based quantitation—and accommodates microvolume adapters (e.g., 50–200 µL) via optional flow cells or capillary holders. It complies with key regulatory frameworks for analytical instrumentation: its firmware architecture supports audit trail generation, user access levels, and electronic signature capability when operated with Agilent ChemStation Rev. A.09.03 or later—facilitating adherence to FDA 21 CFR Part 11, EU Annex 11, and ISO/IEC 17025 documentation standards. All refurbished units include calibration certificates traceable to NIST SRM 2034 (UV-Vis absorbance) and SRM 2035 (wavelength), issued post-reconditioning.
Software & Data Management
Controlled exclusively via Agilent ChemStation software (included with validated license), the system provides method-driven operation, automated validation protocols (e.g., wavelength accuracy, photometric linearity, stray light), and integrated reporting templates aligned with USP , EP 2.2.25, and JP 17 guidelines. Raw spectral data are stored in vendor-neutral .DAD format; export options include ASCII, CSV, and JCAMP-DX for third-party chemometric tools (e.g., MATLAB, Unscrambler). The software enforces role-based permissions (Administrator, Analyst, Reviewer), timestamps all method changes and measurement events, and logs instrument status parameters (lamp hours, temperature, voltage) to support GMP audit readiness.
Applications
- Pharmaceutical QC: Assay determination of active pharmaceutical ingredients (APIs) per USP using fixed-wavelength or multi-point regression
- Environmental analysis: Nitrate/nitrite quantification in water per EPA Method 353.2 and HACH DR3900 cross-validation
- Food & beverage: Color index (AOAC 996.05), anthocyanin profiling, and preservative (e.g., sorbic acid) stability testing
- Academic research: Ligand-binding kinetics, enzyme reaction monitoring (e.g., NADH oxidation), and nanoparticle surface plasmon resonance characterization
- Method transfer: Seamless integration into existing Agilent GC/MS or HPLC-UV workflows via shared ChemStation platform infrastructure
FAQ
Is this instrument supplied with a valid calibration certificate?
Yes—each refurbished Agilent 8453 includes a NIST-traceable calibration report covering wavelength accuracy, photometric accuracy, stray light, and baseline flatness, performed immediately prior to shipment.
What is the expected remaining lamp life after refurbishment?
Deuterium and tungsten-halogen lamps are replaced during refurbishment; typical operational life exceeds 1,000 hours under standard usage conditions.
Does the system support GLP/GMP-compliant data handling?
When operated with ChemStation A.09.03+ and configured with appropriate user roles and audit trail settings, it meets core requirements of 21 CFR Part 11 for electronic records and signatures.
Can I integrate this unit with my existing LIMS or ELN?
Yes—via ChemStation’s OLE/COM interface or ASCII file export, enabling automated data ingestion into most LIMS platforms including LabWare, STARLIMS, and Thermo Fisher SampleManager.
What warranty and technical support are provided?
A 12-month parts-and-labor warranty is included, backed by remote diagnostics and on-site service coordination through Agilent-authorized field engineers in North America and EMEA.
