Aolong ChiRad225 Pro X-ray Irradiator for Radiobiological Research

Overview

The Aolong ChiRad225 Pro X-ray Irradiator is a purpose-built, regulatory-compliant irradiation platform engineered for precision radiobiological research in academic, pharmaceutical, and preclinical laboratories. Operating at a maximum tube voltage of 225 kV, it delivers medium-energy X-rays optimized for uniform dose deposition across small animal models (mice, rats), tissue explants, cell monolayers, microbial cultures, and insect populations. Unlike low-energy (300 kV) radiotherapy-grade units, the 225 kV configuration achieves an optimal depth-dose profile—depositing >90% of its energy within the first 20 mm of biological tissue—making it especially suitable for bone marrow ablation, hematopoietic stem cell transplantation, immunosuppression modeling, and radiosensitivity assays without excessive attenuation or scatter-induced heterogeneity. The system employs a tungsten-target X-ray tube with 2 mm beryllium inherent filtration to harden the beam spectrum and minimize low-energy components that contribute disproportionately to skin dose while offering negligible penetration. All radiation generation, collimation, and dosimetric control are housed within a fully shielded stainless-steel chamber meeting IEC 61010-1 and IEC 62461 safety standards for laboratory X-ray equipment.

Key Features

• Regulatory-ready safety architecture: Dual interlocked doors, emergency stop button, electromagnetic + mechanical locking mechanisms, and real-time leakage monitoring (<0.5 µSv/h at 10 cm).
• Motorized sample handling: Programmable vertical lift and 2 RPM rotation stage ensures dose uniformity across multi-well plates, Petri dishes, and rodent restraint tubes.
• Integrated ionization chamber-based dosimetry: Traceable to national standards; enables position-specific dose verification and real-time exposure feedback during irradiation.
• PLC-controlled operation with Windows-based HMI: Intuitive touchscreen interface supports multi-user password authentication, unlimited protocol storage, and audit-trail-capable session logging.
• Configurable irradiation geometry: Adjustable source-to-object distance (13–80 cm) and interchangeable collimators support both broad-field (40–50 cm Ø) and focused irradiation modes.
• Closed-loop thermal management: Maintains stable tube operating temperature under prolonged duty cycles—critical for reproducible output stability over sequential experiments.

Sample Compatibility & Compliance

The ChiRad225 Pro accommodates diverse biological specimens without modification: standard 6–96-well microplates, T-25/T-75 flasks, 10 cm Petri dishes, mouse/rat restraining tubes (up to 30 cm length), insect cages (e.g., for SIT programs), and cryovial racks. Its chamber interior (75 × 55 × 55 cm) permits simultaneous irradiation of up to 20 mice or 48 T-25 flasks. The system conforms to ISO 4037-1 (X-ray reference radiation fields), ASTM F2582 (radiation sterilization of biomedical products), and supports GLP/GMP-aligned workflows via configurable user access levels, electronic signatures, and 21 CFR Part 11–compliant data export (CSV, XML). Optional calibration certificates traceable to NIM (China) or PTB (Germany) are available upon request.

Software & Data Management

The embedded Windows OS hosts proprietary irradiation control software featuring protocol-driven operation, time/dose/energy parameter locking, and automated sequence execution. Each irradiation session logs operator ID, timestamp, exposure parameters, chamber temperature, and measured dose rate—retained for ≥12 months with optional cloud backup. Export functions generate ISO/IEC 17025-compliant reports including uncertainty budgets for dose delivery. Remote monitoring via Ethernet (TCP/IP) allows integration into centralized lab infrastructure management systems. Software updates are delivered via encrypted USB key or authenticated HTTPS download—no internet connectivity required during routine operation.

Applications

• Preclinical oncology: Fractionated tumor irradiation, radioresistance screening, combination therapy (radiosensitizer/radioprotector evaluation).
• Hematopoietic research: Myeloablation prior to bone marrow transplantation, lineage-specific radiosensitivity profiling (e.g., HSC vs. progenitor cells).
• Immunology: T/B lymphocyte depletion kinetics, dendritic cell maturation studies, graft-versus-host disease (GVHD) modeling.
• Genotoxicity & DNA repair: γH2AX foci quantification, comet assay calibration, ATM/ATR pathway activation studies.
• Microbial radiobiology: Attenuated vaccine development, extremophile radiation resistance characterization, food pathogen inactivation validation.
• Insect genetics: Sterile Insect Technique (SIT) optimization, dominant lethal assay, mutagenesis-driven phenotypic screening.

FAQ

Is the ChiRad225 Pro compliant with international radiation safety regulations?
Yes—the system meets IEC 61010-1 (safety of electrical equipment), IEC 62461 (radiation protection for X-ray equipment), and incorporates design features aligned with national regulatory requirements for Class II radiation devices in the US (FDA 21 CFR 1020.30), EU (Council Directive 2013/59/Euratom), and China (GBZ 138–2002).
Can dose uniformity be validated across large sample batches?
Absolutely—motorized rotation (2 RPM) combined with a calibrated ionization chamber allows spatial mapping per IAEA TRS-398 methodology. Uniformity coefficients ≤±3% across 40 cm Ø field are achievable using the standard turntable configuration.
Does the system support automated throughput enhancement?
Yes—OEM automation interfaces (RS-485, Modbus TCP) enable integration with robotic arms, conveyor-based loading, and LIMS synchronization. Custom chamber extensions and multi-tier sample trays are available as factory-installed options.
What maintenance intervals are recommended for long-term operational reliability?
Annual preventive maintenance includes tube warm-up calibration, dosimeter verification against secondary standard, cooling loop fluid analysis, and interlock function testing—all documented in the included service logbook and accessible via the HMI’s maintenance scheduler.
Is remote technical support available outside mainland China?
Yes—Aolong provides English-language engineering support via secure remote desktop, video-assisted diagnostics, and expedited spare-part logistics through regional distribution partners in North America, EMEA, and APAC.