Chengmeng HYL-B Reciprocating Full-Temperature Orbital Shaking Incubator

Overview

The Chengmeng HYL-B Reciprocating Full-Temperature Orbital Shaking Incubator is an integrated benchtop-to-floor-standing platform engineered for precise, reproducible cultivation of microbial, mammalian, and suspension cell cultures under controlled temperature and dynamic agitation conditions. Unlike standard orbital shakers, the HYL-B employs a reciprocating (linear back-and-forth) motion mechanism—distinct from rotational or elliptical orbits—optimized for high-oxygen-transfer applications such as aerobic fermentation, protein expression in E. coli, and mycelial growth in fungal bioprocessing. Its full-temperature range (5–60 °C), coupled with a high-efficiency Tecumseh compressor and PID-controlled refrigeration-heating system, enables stable thermal regulation across ambient fluctuations, supporting both cold-adapted psychrophilic strains and thermophilic cultures. The unit complies with fundamental mechanical safety standards for laboratory equipment (IEC 61010-1) and meets structural requirements for continuous-duty operation in ISO 17025-accredited QC labs and GLP-compliant R&D environments.

Key Features

• Reciprocating oscillation drive with continuously adjustable frequency (40–280 rpm) and programmable auto-hold functionality ensures consistent shear profile across extended runs.
• Selectable stroke amplitude (50–80 mm) allows optimization of oxygen transfer rate (OTR) and mixing efficiency for diverse vessel geometries—from narrow-neck Erlenmeyer flasks to wide-mouth bioreactor bottles.
• Dual-mode temperature control: Peltier-assisted cooling and resistive heating maintain ±0.3 °C stability at setpoint across the full 5–60 °C range, verified per ASTM E2500 Annex A guidelines for incubator qualification.
• Microprocessor-based LED controller with real-time display of setpoint/actual temperature, speed, and remaining time; supports parameter locking to prevent accidental modification during unattended operation.
• Brushless DC drive motor delivers low-vibration, low-noise performance (<55 dB(A) at 1 m), extends service life beyond 20,000 hours, and eliminates carbon brush wear—critical for GMP-aligned cleanroom-adjacent installations.
• Single-tier reinforced stainless-steel shaking platform (1300 × 800 mm) accommodates up to 70 × 250 mL flasks or 18 × 1000 mL vessels; platform surface features non-slip rubber matting and universal flask clamps compatible with standard ISO 4796-1 neck dimensions.

Sample Compatibility & Compliance

The HYL-B accommodates standard glass and PETG culture vessels ranging from 50 mL test tubes to 1000 mL baffled flasks, with optional custom fixture kits available for microplate carriers (96-/384-well) and roller bottle adapters. Its mechanical design conforms to ISO 22196 for antimicrobial surface testing protocols when used with sterile enclosures. While not certified for IEC 61326-1 EMC compliance out-of-box, it operates within typical lab electromagnetic noise thresholds and may be deployed in shared instrumentation rooms when installed ≥1 m from sensitive analytical devices (e.g., mass spectrometers). For regulated biomanufacturing workflows, the timer function supports ALCOA+ data integrity principles when paired with external audit-trail logging via RS-485 interface (optional module).

Software & Data Management

The HYL-B operates as a standalone instrument with embedded firmware; no proprietary PC software is required for basic operation. However, its RS-485 serial port (Modbus RTU protocol) enables integration into centralized Lab Information Management Systems (LIMS) or SCADA platforms for remote monitoring of temperature, speed, and runtime. Time-stamped operational logs—including start/stop events, parameter changes, and fault codes—are retained in non-volatile memory for up to 30 days. When connected to validated third-party data acquisition software compliant with FDA 21 CFR Part 11 (e.g., LabArchives or DeltaV), the unit supports electronic signatures, role-based access control, and immutable audit trails—facilitating regulatory submissions for clinical trial material production.

Applications

• Aerobic microbial culture expansion for recombinant protein production (e.g., BL21(DE3) in TB medium).
• Secondary metabolite screening in actinomycetes and filamentous fungi requiring high dissolved oxygen tension.
• Seed train preparation for single-use bioreactors, including inoculum scaling from shake flasks to 5-L wave bags.
• Environmental microbiology studies involving soil slurry enrichment or wastewater bioaugmentation trials.
• Cell line adaptation protocols where gradual shear stress modulation improves suspension viability (e.g., CHO-S passaging).
• Standardized method development per USP Sterility Tests and EP 2.6.27 Microbial Enumeration Tests, where consistent agitation is critical for recovery efficiency.

FAQ

What is the minimum ambient temperature required for stable operation at 5 °C?
The unit achieves and maintains 5 °C only when ambient room temperature does not exceed 25 °C; at higher ambient temperatures, the lowest achievable setpoint increases due to compressor thermal load limitations.
Can the HYL-B accommodate stacked multi-tier configurations?
No—the HYL-B is engineered exclusively for single-shelf operation. Multi-tier stacking is not supported structurally or thermally; alternative models (e.g., HYL-C series) are available for vertical capacity expansion.
Is the shaking platform autoclavable?
The stainless-steel platform itself is not autoclavable due to integrated wiring and sensor components; however, it is fully cleanable with 70% ethanol, isopropyl alcohol, or 0.5% sodium hypochlorite solution per ISO 14644-1 Class 5 cleaning protocols.
Does the timer support repeat-cycle programming?
No—the built-in timer is a single-interval countdown (0–999 min); for multi-stage protocols (e.g., heat-shock + recovery), external programmable logic controllers (PLCs) or LIMS-triggered relay modules are recommended.
What calibration documentation is provided upon shipment?
Each unit ships with a factory-verified temperature and speed calibration certificate traceable to NIM (National Institute of Metrology, China), including as-found/as-left data points at three setpoints (10 °C, 37 °C, 55 °C) and two speeds (60 rpm, 200 rpm).