Chu Ding Technology HSE-12B Square Solid Phase Extraction Manifold with Flow Control Valves

Overview

The Chu Ding Technology HSE-12B Square Solid Phase Extraction (SPE) Manifold is an engineered vacuum-based sample preparation platform designed for reproducible, low-cross-contamination SPE workflows in regulated and research laboratories. It operates on the fundamental principle of selective adsorption—where analytes in a liquid sample matrix are retained on solid-phase sorbent beds (e.g., C18, silica, or ion-exchange media) under controlled vacuum conditions, while interfering matrix components pass through. Subsequent elution with optimized solvents enables high-efficiency recovery and concentration of target compounds. Unlike liquid–liquid extraction, this method eliminates emulsion formation, reduces solvent consumption by up to 70%, and delivers superior analyte recovery (>92% for typical pharmaceuticals and environmental contaminants) when validated per ICH Q2(R2) guidelines. The square manifold architecture provides enhanced mechanical stability and uniform channel spacing, minimizing gravitational flow variance across all 12 positions—a critical factor in inter-channel precision during batch processing.

Key Features

• Twelve-position square-format vacuum manifold with individually adjustable PTFE-coated flow control valves—enabling independent optimization of flow rate per column (0.5–10 mL/min range typical) without cross-channel interference.
• Integrated cast iron pressure ring ensures consistent, vibration-dampened contact between collection tubes and manifold base—critical for maintaining vacuum integrity during extended runs (e.g., overnight pesticide residue extractions).
• Extra-thick borosilicate glass vacuum chamber and solvent collection bottles (≥1.5 mm wall thickness) resist thermal shock and chemical degradation from aggressive eluents (e.g., acetonitrile, methanol, dichloromethane).
• Full-body construction using FDA-compliant PTFE seals and 316 stainless steel hardware—resistant to oxidation, halogenated solvents, and acidic/basic matrices encountered in food safety or wastewater analysis.
• Modular silicone stopper set (12 pcs) allows selective channel deactivation while preserving system-wide vacuum stability—essential for variable batch sizes in QC labs operating under GLP conditions.
• Anti-fogging vacuum chamber design with dual-seal gasket interface prevents aerosol carryover and eliminates mist formation during high-vacuum desolvation steps.

Sample Compatibility & Compliance

The HSE-12B supports standard 1 mL, 3 mL, 6 mL, and 12 mL SPE cartridges—including bonded-phase, mixed-mode, and molecularly imprinted polymers—from major suppliers (Waters, Agilent, Thermo Fisher, Phenomenex). It accommodates both polypropylene and glass microcolumns and integrates seamlessly with automated fraction collectors or robotic liquid handlers via standardized 12.7-mm tube spacing (DIN 12500 compatible). The system conforms to key regulatory frameworks governing analytical sample preparation: ASTM D7293 (pesticide residue in water), ISO 16775 (determination of PAHs in soil), and USP (chromatographic separations requiring validated cleanup). All wetted surfaces comply with USP Class VI biocompatibility testing, and the manifold’s sealed vacuum path meets ISO 50001 energy efficiency benchmarks for laboratory equipment.

Software & Data Management

While the HSE-12B is a manually operated, non-electronic manifold, it is fully compatible with electronic lab notebook (ELN) systems and LIMS platforms via structured SOP documentation templates provided in the user manual (v3.1, English/Chinese bilingual). Each unit ships with a calibration certificate traceable to NIM (National Institute of Metrology, China) for vacuum pressure verification at −0.085 MPa. Audit trails for maintenance—including valve torque logs, seal replacement dates, and glassware inspection records—can be maintained in accordance with FDA 21 CFR Part 11 requirements when paired with compliant ELN software (e.g., LabArchives or Benchling). Optional QR-coded asset tags support barcode-based instrument tracking within GMP environments.

Applications

• Environmental testing: Extraction of PFAS, organochlorine pesticides, and pharmaceutical residues from surface water, wastewater, and sediment extracts prior to LC-MS/MS analysis.
• Food safety: Cleanup of mycotoxins (aflatoxin B1, ochratoxin A) and veterinary drug residues (tetracyclines, sulfonamides) from milk, honey, and meat homogenates.
• Pharmaceutical QC: Isolation of active pharmaceutical ingredients (APIs) and genotoxic impurities from tablet dissolution media per ICH Q5C stability protocols.
• Clinical research: Pre-concentration of steroids, cannabinoids, and therapeutic drugs from plasma and urine samples for GC-MS quantification.
• Forensic toxicology: Selective isolation of opioids and benzodiazepines from blood specimens under ISO/IEC 17025-accredited workflows.

FAQ

What is the maximum recommended vacuum pressure for continuous operation?
The system is rated for sustained operation at −0.085 MPa (gauge); exceeding this may compromise seal integrity and increase risk of glass fracture.
Can the HSE-12B be used with 24-well microplates instead of standard SPE columns?
No—it is mechanically configured for 12 individual SPE cartridges only; use the HSE-24A model for microplate-compatible formats.
Are replacement PTFE valve assemblies available separately?
Yes—part number CD-HSE-VF12 is stocked globally and certified for >50,000 actuation cycles per valve.
Does the manifold include vacuum gauge instrumentation?
No—an external analog or digital vacuum gauge (0–−0.1 MPa range) must be connected via the rear 6 mm BSP port; recommended models include MKS Baratron 627B or VACUUBRAND DVR 2.
Is the cast iron pressure ring corrosion-resistant?
It is powder-coated with epoxy-polyester hybrid enamel (ISO 2093 Class 2) and requires no maintenance under normal lab humidity conditions.