Chubai DG-24 Automated Solid Phase Extraction System

Overview

The Chubai DG-24 Automated Solid Phase Extraction System is an engineered solution for high-throughput, reproducible sample preparation in analytical laboratories. Based on membrane-supported solid-phase extraction (SPE) principles, the system leverages controlled vacuum-driven flow through chemically functionalized membranes or packed sorbent cartridges to selectively retain target analytes while removing matrix interferences. Unlike conventional column-based SPE, the DG-24’s membrane architecture enables rapid equilibration, reduced channel clogging, and consistent flow dynamics across all ports—critical for method robustness in regulated environments. Designed for routine use in environmental monitoring, food safety testing, pharmaceutical QC, and clinical toxicology workflows, the instrument supports standardized protocols aligned with EPA Method 525.3, ISO 17025-compliant validation requirements, and GLP-aligned documentation practices.

Key Features

• Fully automated operation with programmable vacuum pressure regulation and timed step sequencing (conditioning, loading, washing, elution, and drying)
• Multi-channel configuration (standard 12-port; optional 24/36-port trays) enabling parallel processing of multiple samples without manual intervention
• Membrane-based SPE compatibility—optimized for hydrophilic-lipophilic balanced (HLB), C18, silica, and ion-exchange membranes as well as traditional packed-bed cartridges
• Integrated flow path isolation to prevent cross-contamination between channels; each port features independent sealing and pressure feedback
• Compact benchtop footprint (165 × 125 × 175 mm) with lightweight aluminum alloy chassis and chemical-resistant fluidic pathways
• Universal sample vessel support—including 12–16 mm OD glass/test tubes, 1.5–15 mL centrifuge tubes, and standard autosampler vials—eliminating need for adapter kits
• Single-solvent elution module with precise metering and dwell-time control, suitable for gradient-compatible methods when paired with external solvent switching valves

Sample Compatibility & Compliance

The DG-24 accepts liquid samples directly—including aqueous extracts, plasma supernatants, urine digests, and food homogenates—as well as semi-solid matrices pre-dissolved in compatible solvents. Solid samples must undergo prior homogenization and solvent extraction per AOAC 2007.01 or EN 15662 guidelines before SPE application. The system complies with general laboratory safety standards (IEC 61010-1) and supports audit-ready workflows under FDA 21 CFR Part 11 when operated with validated third-party LIMS or ELN software. All wetted materials—including tubing, manifolds, and sealing gaskets—are certified non-leaching and inert to common organic solvents (methanol, acetonitrile, ethyl acetate, dichloromethane) and aqueous buffers (pH 2–10). No internal pumps or moving parts contact solvents—vacuum is supplied externally, preserving long-term calibration stability.

Software & Data Management

The DG-24 operates via front-panel push-button interface with LED status indicators for real-time monitoring of vacuum level, elapsed time per step, and cycle completion. While the base unit does not include embedded PC software, it provides TTL-level trigger outputs and RS-232 serial communication for integration into centralized lab automation systems. Users may log run parameters manually or export timestamped cycle logs via optional USB data logger modules. For full electronic recordkeeping, the system interfaces seamlessly with commercial chromatography data systems (CDS) such as Thermo Fisher Chromeleon, Agilent OpenLab CDS, or Waters Empower—enabling traceable linkage between SPE run IDs and subsequent LC-MS/MS or GC-FID analyses. Audit trails, user access levels, and electronic signatures are implemented at the host software layer per ALCOA+ principles.

Applications

• Environmental analysis: Extraction and concentration of PAHs, PCBs, phthalates, and organochlorine pesticides from drinking water, wastewater, and soil leachates
• Food safety: Multi-residue screening of neonicotinoid insecticides, triazole fungicides, and glyphosate metabolites in fruits, vegetables, and infant formula
• Pharmaceutical QC: Clean-up of dissolution media and stability samples prior to HPLC-UV quantification of active pharmaceutical ingredients (APIs)
• Clinical toxicology: Isolation of benzodiazepines, opioids, and stimulants from human serum and urine using mixed-mode cation-exchange SPE membranes
• Forensic chemistry: Pre-concentration of cannabinoids and synthetic cathinones from oral fluid specimens in accordance with SWGTOX recommendations

FAQ

What types of SPE sorbents are compatible with the DG-24?
The system supports all standard 1-mL and 3-mL SPE cartridges and disk formats—including bonded silica phases (C8, C18, NH₂, CN), polymeric resins (HLB, MAX, MCX), and inorganic media (silica, Florisil)—provided they fit within the specified height and diameter constraints.
Can the DG-24 perform sequential solvent elution?
The base configuration uses a single eluent line; sequential or gradient elution requires external valve switching (e.g., Rheodyne 7725i) connected via the system’s vacuum manifold outlet.
Is method validation support available?
Chubai provides basic IQ/OQ documentation templates and performance verification checklists (e.g., recovery tests using spiked water standards); full PQ validation must be conducted by the end user per ISO/IEC 17025 Section 5.4.5.
What maintenance is required?
Routine maintenance includes periodic inspection of O-rings, cleaning of the vacuum manifold with isopropanol, and replacement of the external vacuum pump oil every 500 operating hours.
Does the DG-24 meet regulatory requirements for GMP labs?
As a Class I laboratory device, it qualifies as ancillary equipment under EU Annex 11 and FDA guidance when integrated into a validated workflow with appropriate change control, calibration records, and operator training documentation.