CoMetro R1/R2 Automated Fraction Collector
| Brand | CoMetro |
|---|---|
| Model | R1/R2 |
| Type | Mixed-Mode Fraction Collector |
| Collection Principle | Volume-Based, Drop-Counting, Time-Based, and Peak-Triggered |
| Max Flow Rate | 100 mL/min |
| Dimensions (R1) | 31 × 33 × 35.5 cm |
| Dimensions (R2) | 31 × 53 × 38 cm |
| Weight (R1, excl. rack) | 7.7 kg |
| Weight (R2, excl. rack) | 10.6 kg |
| Power Supply | 115 VAC or 230 VAC, 50/60 Hz |
| Tube Capacity Options | Up to 144 × 13 mm tubes, 100 × 16 mm tubes, 72 × 18 mm tubes, 36 × 25 mm tubes, 60 × 1.5 mL microcentrifuge tubes, 36 × 50 mL centrifuge tubes, or 96-well plates |
| Standard Configurations | R1 — single rack + valve |
Overview
The CoMetro R1/R2 Automated Fraction Collector is a precision-engineered chromatography accessory designed for integration with preparative liquid chromatography (prep-LC), gravity-fed column chromatography, and fast protein liquid chromatography (FPLC) systems. It operates on a modular, programmable collection architecture that supports four independent triggering modes—volume-based, drop-counting, time-based, and peak-triggered collection—enabling robust fractionation across diverse separation workflows. Its core fluidic design employs a high-reproducibility peristaltic or pressure-driven flow path (depending on system configuration), coupled with a low-dead-volume switching valve to minimize carryover and ensure sample integrity. The device is engineered for GLP-compliant environments where traceability, repeatability, and minimal cross-contamination are critical—particularly in natural product isolation, peptide purification, metabolite enrichment, and pharmaceutical process development.
Key Features
- Multi-mode collection logic: configurable by volume (mL), time (s/min), drop count (user-defined drop volume calibration), or external analog/digital signal input (e.g., UV/VIS detector peak output)
- Dual-collection configurations: R1 supports single-rack operation (up to 144 fractions); R2 extends capacity via dual independent rack positioning, enabling staggered or parallel collection strategies
- Low-noise, brushless DC motor drive ensures stable valve actuation and sub-second tube-switching latency (< 300 ms typical), preserving peak resolution and recovery yield
- Integrated electrical safety architecture includes leakage current protection (IEC 61010-1 compliant) and reinforced insulation against moisture ingress—critical for solvent-rich lab environments
- Modular rack system accommodates standardized tube formats: 13 mm, 16 mm, 18 mm, and 25 mm diameter glass or plastic tubes; 1.5 mL and 50 mL microcentrifuge tubes; and ANSI/SLAS-standard 96-well plates
- Front-panel interface with LCD display and rotary encoder allows standalone method setup, real-time status monitoring, and emergency stop activation without PC dependency
Sample Compatibility & Compliance
The R1/R2 collector is compatible with common HPLC/FPLC solvents—including aqueous buffers, acetonitrile, methanol, isopropanol, and chloroform—provided tubing and valve seals are chemically rated (e.g., PTFE, Chemraz®, or EPDM). All wetted materials comply with USP Class VI biocompatibility standards. The unit meets IEC 61326-1 (EMC for laboratory equipment) and IEC 61010-1 (safety requirements for electrical equipment for measurement, control, and laboratory use). Optional audit trail logging (via RS-232 or USB) supports 21 CFR Part 11–aligned data integrity when paired with validated third-party acquisition software.
Software & Data Management
While fully functional in stand-alone mode, the R1/R2 supports bidirectional serial communication (RS-232) and USB virtual COM port protocols for seamless integration with third-party chromatography data systems (CDS), including Chromeleon™, Empower™, and OpenLab CDS. Method files—including collection thresholds, rack mapping, pause/resume logic, and post-collection alerts—are stored in non-volatile memory (≥100 methods). Firmware updates are performed via USB flash drive, with version rollback capability. No proprietary software installation is required; configuration is accessible via ASCII command set documented in the technical manual.
Applications
- Preparative purification of monoclonal antibodies and recombinant proteins under GMP-aligned conditions
- Isolation of bioactive natural compounds from crude plant extracts using gradient elution
- Automated collection of SEC (size-exclusion chromatography) fractions for multi-angle light scattering (MALS) analysis
- Time-resolved metabolite profiling in LC-MS workflows requiring precise fraction timing
- High-throughput screening sample preparation for NMR or HRMS, where consistent volume allocation per well is essential
- Method development support for ion exchange and hydrophobic interaction chromatography (HIC)
FAQ
What types of detectors can trigger peak-based collection?
The R1/R2 accepts TTL-level or 0–10 V analog signals from UV-Vis, conductivity, pH, or mass spectrometry detectors—no signal conditioning required.
Can I use non-standard tube racks?
Yes. Custom rack interfaces are available under OEM agreement; mechanical drawings and mounting specifications are provided upon request.
Is the switching valve compatible with aggressive solvents like DMSO or THF?
Standard valve configuration uses Chemraz® O-rings and PTFE rotor seals, rated for continuous exposure to DMSO, THF, DMF, and dichloromethane at ambient temperature.
Does the device support fractional collection during gradient runs with changing flow rates?
Yes—volume-based collection remains accurate regardless of flow rate fluctuation, as it relies on calibrated pulse counting from an integrated flow sensor (optional accessory) or external flow meter input.
How is calibration traceability maintained?
Each unit ships with a factory calibration certificate referencing NIST-traceable volumetric standards; user recalibration procedures are detailed in the service manual and require only certified syringes and stopwatch verification.
