DELEITE V8-10 8-Channel Manual Pipette

Overview

The DELEITE V8-10 is an 8-channel manual pipette engineered for high-reproducibility liquid handling in standardized microplate workflows—particularly 96-well and 384-well formats. It operates on the air displacement principle, where mechanical plunger actuation creates a controlled vacuum within a sealed piston chamber, drawing and dispensing liquid through disposable polypropylene tips. Designed for routine molecular biology, ELISA, cell culture seeding, and assay setup applications, the V8-10 delivers trace-level precision in the sub-10 µL range—a critical performance tier where surface tension, vapor pressure, and tip geometry exert dominant influence on volumetric fidelity. Its all-metal gear-driven mechanism ensures consistent plunger resistance and tactile feedback across thousands of cycles, minimizing operator-induced variability. The device complies with ISO 8655-2 (2022) requirements for manual piston-operated pipettes, including specifications for calibration methodology, error tolerance definition, and metrological traceability.

Key Features

• Eight independently aligned channels with synchronized tip ejection and volume adjustment—ensuring parallel aspiration/dispense across full rows of standard microplates.
• Precision-machined stainless-steel plunger system with dual O-ring sealing, rated for ≥100,000 operational cycles under GLP-compliant usage conditions.
• Volume adjustment dial calibrated to 0.1 µL increments over the 0.5–10 µL range, enabling fine-tuned optimization for low-volume PCR master mixes or antibody dilutions.
• Ergonomic, low-force activation lever (≤1.8 N at full stroke) reduces repetitive strain during high-throughput plate filling protocols.
• Interchangeable tip cone design compatible with universal 10 µL low-retention filter tips meeting ISO 8655-6 specifications for residual volume and hydrophobicity control.
• Integrated volume lock prevents accidental setting drift during transport or multi-user lab environments.

Sample Compatibility & Compliance

The V8-10 supports aqueous buffers, saline solutions, glycerol-diluted reagents (up to 15% v/v), and low-viscosity cell suspensions (e.g., PBS-washed mammalian cells at ≤1×10⁶/mL). It is not intended for viscous, volatile, or corrosive liquids (e.g., concentrated acids, DMSO >30%, or organic solvents). All models are manufactured in accordance with GB/T 27476.5–2014 (Chinese national standard equivalent to ISO/IEC 17025 for calibration laboratories) and include factory calibration certificates traceable to NIM (National Institute of Metrology, China). While not certified for IVD or clinical diagnostics use, the pipette meets general laboratory quality requirements under ISO/IEC 17025:2017 Annex A.2 for equipment verification prior to first use and periodic recalibration every 6–12 months depending on usage frequency and criticality of application.

Software & Data Management

As a fully manual instrument, the V8-10 does not incorporate onboard electronics or digital interfaces. However, it integrates seamlessly into digitally governed lab environments via external documentation systems. Each unit ships with a unique serial number and accompanying calibration record (PDF format), supporting integration into LIMS platforms such as LabVantage or STARLIMS through manual import or barcode-scanned metadata capture. For FDA 21 CFR Part 11 compliance, users may assign electronic signatures to calibration logs using validated PDF annotation tools; audit trails must be maintained separately per institutional SOPs. Routine performance verification (e.g., gravimetric testing per ISO 8655-5) is recommended before each shift when used in regulated workflows requiring documented accuracy assurance.

Applications

• High-throughput qPCR and RT-qPCR plate setup, including template, primer, and master mix dispensing at volumes between 1–10 µL.
• ELISA plate coating and detection antibody dilution series generation with ≤2.5% coefficient of variation across replicates.
• CRISPR guide RNA complex assembly where stoichiometric ratios demand sub-microliter precision.
• Microbial colony picking and suspension normalization in 96-deep-well plates for antibiotic susceptibility screening.
• Cell-based assay seeding consistency validation—e.g., uniform delivery of 5 µL of 2×10⁴ cells/mL suspension into assay plates.

FAQ

Is the V8-10 suitable for use with filtered tips?
Yes—the tip cone accepts standard 10 µL universal filtered tips conforming to ISO 8655-6 dimensional tolerances and hydrophobic barrier integrity specifications.
What is the recommended recalibration interval?
Every 6 months for daily high-volume use (>50 plates/day); annually for intermittent use (<5 plates/week), provided gravimetric verification passes per ISO 8655-5 Annex B.
Can this pipette be sterilized in an autoclave?
No—only the removable tip cones may be autoclaved at 121°C for 20 minutes; the main body contains lubricants and elastomers incompatible with steam sterilization.
Does DELEITE provide ISO 17025-accredited calibration services?
Yes—third-party accredited calibration is available upon request through authorized service centers in Shanghai, Beijing, and Guangzhou, with certificates issued against CNAS-CL01:2018 requirements.
How is accuracy verified at the 5 µL test point?
Via gravimetric method using Class A analytical balance (±0.001 mg resolution), temperature-controlled distilled water (20 ± 0.5°C), and ISO 8655-5 defined procedure—including 10 replicate measurements, outlier rejection per Grubbs’ test, and expanded uncertainty calculation (k=2).