DLAB TopPette Single-Channel Adjustable-Volume Manual Pipette

Overview

The DLAB TopPette Single-Channel Adjustable-Volume Manual Pipette (Model: 5–50 µL) is an ISO 8655-compliant precision liquid handling instrument engineered for reproducible volumetric delivery in routine and regulated laboratory environments. Utilizing an air displacement principle with a high-stability piston-cylinder mechanism, this pipette delivers consistent aspiration and dispensing across its full range—optimized for applications requiring trace-level accuracy in molecular biology, clinical diagnostics, pharmaceutical QC, and academic research. Its design adheres to internationally recognized metrological standards for gravimetric performance verification (e.g., ISO 8655-2, ISO 8655-5), ensuring traceability and compatibility with GLP/GMP documentation workflows. The instrument features a fully autoclavable lower assembly—including tip cone, sleeve, and O-rings—validated for repeated sterilization without degradation of sealing integrity or dimensional stability.

Key Features

• Ergonomic Actuation System: Optimized plunger spring force and tactile feedback reduce hand fatigue during high-frequency operations; average actuation force is ≤1.8 N at full volume, meeting ISO 8655-6 ergonomic thresholds.
• High-Contrast Volume Display: Dual-scale digital window enables rapid verification of set volume and fine adjustment increments (0.5 µL resolution), with parallax-free viewing from multiple angles.
• Chemical Resistance & Sterilization: Lower assembly constructed from PTFE-coated stainless steel and chemically inert thermoplastics; withstands ≥20 cycles of steam sterilization at 121 °C/103 kPa without seal swelling or calibration drift.
• Contamination Control: Optional integrated aerosol barrier filter (replaceable polypropylene hydrophobic membrane) prevents sample carryover and protects internal mechanisms from corrosive vapors or viscous liquids.
• Modular Service Architecture: Fully serviceable design with tool-free disassembly of tip cone, piston, and seal housing; individual channel components are interchangeable across the TopPette series (0.1–5000 µL).
• Volume Lock Mechanism: Mechanical lock prevents accidental volume changes during transport or multi-step protocols, enhancing procedural consistency in shared lab environments.

Sample Compatibility & Compliance

The TopPette 5–50 µL pipette is validated for aqueous solutions, buffers, diluted acids/bases (pH 2–12), and common organic solvents including ethanol, isopropanol, and acetonitrile (with appropriate tip selection). It complies with ISO 8655-2 (volumetric performance), ISO 8655-5 (calibration procedures), and supports audit-ready documentation per FDA 21 CFR Part 11 when used with DLAB’s optional calibration certificate package (including as-found/as-left data, uncertainty budget, and technician signature). All materials meet USP Class VI biocompatibility requirements. The pipette is CE-marked under Directive 2014/30/EU (EMC) and 2014/35/EU (LVD), and conforms to RoHS 2011/65/EU restrictions on hazardous substances.

Software & Data Management

While the TopPette 5–50 µL is a manual pipette and does not incorporate embedded electronics or connectivity, DLAB provides a comprehensive offline calibration management suite—TopCal™ v3.2—for laboratories maintaining electronic records. TopCal supports creation of calibration schedules, generation of ISO/IEC 17025-compliant certificates (with expanded uncertainty calculations per GUM), and export of audit logs in CSV/PDF formats compatible with LIMS integration. Each pipette ships with a unique serial number engraved on the body, enabling asset-level tracking and traceability across calibration cycles. For labs operating under 21 CFR Part 11, TopCal offers user authentication, electronic signatures, and immutable audit trails for all calibration events.

Applications

• Preparation of qPCR master mixes and NGS library dilutions requiring sub-microliter precision
• Serial dilution workflows in ELISA and cell-based assay development
• Standard curve preparation for HPLC and LC-MS quantitation
• Reagent dispensing in microplate-based high-content screening (HCS)
• Quality control testing of raw materials and finished products in ISO 13485-certified medical device manufacturing
• Teaching laboratories where robustness, ease of maintenance, and student-safe ergonomics are prioritized

FAQ

Is the TopPette 5–50 µL pipette suitable for use with volatile organic solvents?
Yes—when equipped with the optional solvent-resistant seal kit (DLAB P-SEAL-SOLV), it maintains accuracy and longevity with solvents such as acetone, chloroform, and DMSO. Standard seals are rated for alcohols and aqueous media only.
Can this pipette be calibrated in-house without specialized equipment?
Basic field calibration is possible using DLAB’s certified calibration kit (Cat. No. CAL-KIT-TOP), which includes gravimetric standards, distilled water, analytical balance (0.01 mg resolution), and step-by-step SOPs aligned with ISO 8655-5.
What is the recommended recalibration interval?
DLAB recommends quarterly calibration for daily use in regulated environments; semi-annual calibration is acceptable for low-throughput academic settings. Frequency must be justified by risk assessment per ISO/IEC 17025 Clause 7.7.
Does the pipette include a certificate of conformity?
Each unit ships with a factory-issued CoC referencing ISO 8655 compliance and material declarations. A UKAS-accredited calibration certificate is available upon request at time of order.
Are replacement parts available globally?
Yes—DLAB maintains a Tier-1 global spare parts network with 48-hour dispatch for critical components (plungers, seals, tip cones) from regional hubs in Rotterdam, Chicago, and Singapore.