DRETOP DZYL.CP-7145 CO₂ Incubator Shaker

Overview

The DRETOP DZYL.CP-7145 CO₂ Incubator Shaker is an integrated bioprocessing platform engineered for precise simultaneous control of temperature, CO₂ concentration, and orbital agitation. It operates on the principle of forced convection heating combined with infrared-based CO₂ sensing and microprocessor-regulated motor-driven oscillation—enabling stable physiological conditions for aerobic cell culture, microbial fermentation, hybridization assays, and enzymatic reaction kinetics under controlled gaseous environments. Designed specifically for mammalian cell suspension cultures, bacterial expression systems, and tissue-derived primary cultures requiring both gas-phase regulation and mechanical stimulation, this instrument bridges the functional gap between standalone CO₂ incubators and benchtop shakers—eliminating cross-contamination risks associated with external transfer and reducing footprint by up to 40% compared to modular setups.

Key Features

• Microprocessor-controlled PID regulation for temperature (±0.1°C accuracy) and rotational speed (±1 rpm stability), with real-time display of setpoints and actual values on a backlit LCD interface;
• Dual-tier stainless steel shelves (710 × 455 mm each), compatible with standard Erlenmeyer flasks, culture bottles, and multi-well plates—supporting scalable workflows from R&D to pilot-scale cultivation;
• Infrared CO₂ sensor (0–20% range, ±0.1% resolution) paired with a high-efficiency 0.3 µm particulate filter at the gas inlet, ensuring sterility and long-term calibration stability;
• Brushless DC motor drive system delivering consistent torque across the full 0–300 rpm range, with soft-start acceleration to prevent splashing and maintain sample integrity during initiation;
• Three-dimensional eccentric drive mechanism with reinforced bearing assembly, enabling uniform orbital motion (26 mm diameter) and minimizing vibration transmission to adjacent equipment;
• Integrated safety architecture including over-temperature cutoff, overspeed shutdown, door-open interlock, automatic restart after power interruption, and user-configurable parameter lockout;
• Optional UV germicidal lamp (254 nm) for periodic decontamination of chamber air and interior surfaces, compliant with ISO 14644-1 Class 5 cleanroom maintenance protocols.

Sample Compatibility & Compliance

The DZYL.CP-7145 accommodates common laboratory vessels—including 250 mL to 5000 mL glass or PETG flasks, roller bottles, and stackable culture containers—without compromising gas exchange or thermal homogeneity. Its chamber design conforms to ASTM E1545-21 guidelines for incubator performance verification and supports GLP-compliant operation when used with optional data logging modules. The CO₂ delivery system meets ISO 8573-1:2010 Class 2 purity standards for compressed gases in life science applications. All internal surfaces are electropolished 304 stainless steel, facilitating cleaning validation per FDA 21 CFR Part 11 requirements when paired with audit-trail-enabled software.

Software & Data Management

While the base unit features embedded firmware with non-volatile memory for parameter retention, optional USB data export enables traceable recordkeeping of temperature, CO₂ concentration, RPM, and runtime. Exported CSV files are compatible with LIMS integration and support statistical process control (SPC) analysis. For regulated environments, the optional DRETOP LabLink™ software package provides electronic signature capability, change control logs, and 21 CFR Part 11–compliant audit trails—including operator ID, timestamped events, and modification history. Remote monitoring via Ethernet is available through OEM-integrated IoT gateways supporting MQTT/OPC UA protocols.

Applications

• Mammalian cell line expansion under 5% CO₂ for monoclonal antibody production;
• High-density E. coli fermentation with pH-coupled DO control (when interfaced with external probes);
• Yeast two-hybrid screening requiring synchronized shaking and ambient CO₂ stabilization;
• Stem cell differentiation assays where mechanical stimulation synergizes with gas-regulated metabolic priming;
• Environmental microbiology studies involving CO₂-responsive soil isolates or methanogenic consortia;
• Biopharmaceutical process development adhering to ICH Q5D and Q5A quality-by-design (QbD) frameworks.

FAQ

What CO₂ concentration ranges can the DZYL.CP-7145 maintain, and how is calibration performed?

The unit maintains CO₂ levels from 0% to 20% using factory-calibrated NDIR sensors; field recalibration is supported via zero-air and span-gas procedures per ISO 13485 Annex A.
Is the chamber humidity controlled?

No—this model does not include active humidification; users requiring >60% RH should consider the ZHL-RH18 series or integrate external humidified gas lines.
Can the dual shelves operate at independent temperatures or speeds?

No—both platforms share identical setpoints for temperature, CO₂, and oscillation parameters to ensure chamber-wide uniformity.
What certifications does the device hold for international shipment?

CE marking (2014/30/EU EMC Directive and 2014/35/EU LVD Directive), RoHS 2011/65/EU compliance, and CB Scheme test reports per IEC 61010-1:2010.
Are third-party validation documents available for IQ/OQ/PQ protocols?

Yes—DRETOP provides customizable URS templates, FAT/SAT checklists, and protocol-ready test scripts upon request for GMP-aligned installations.