DRETOP ZYL.CP-5036R CO₂-Enabled Refrigerated Orbital Shaker Incubator

Overview

The DRETOP ZYL.CP-5036R is a dual-platform, refrigerated orbital shaker incubator engineered for precise, long-term cultivation of mammalian cells, suspension cultures, and CO₂-dependent microorganisms under tightly controlled environmental conditions. Unlike conventional shakers or standalone CO₂ incubators, this integrated system combines active temperature regulation (including sub-ambient cooling), programmable orbital agitation, and real-time CO₂ concentration control—enabling simultaneous maintenance of physiological pH (via bicarbonate buffering) and optimal metabolic activity during dynamic culture processes. Its operation relies on Peltier-assisted refrigeration coupled with high-efficiency compressor-based cooling (R-series configuration), PID-controlled heating, and non-dispersive infrared (NDIR) CO₂ sensing to sustain stable gas composition (0–20% v/v) across the chamber volume. Designed for GLP-compliant labs, it supports reproducible growth kinetics studies, bioprocess optimization, and routine cell passaging where thermal stability, mechanical consistency, and atmospheric fidelity are critical.

Key Features

• Dual independent stainless-steel shaking platforms (500 × 360 mm each), enabling parallel experiments with differential speed or timing protocols
• PID-controlled temperature regulation from 4 °C to 60 °C with ±0.1 °C accuracy and <±0.3 °C uniformity across platform surface
• Orbital motion driven by brushless DC motor with 0–300 rpm range, ±1 rpm speed stability, and soft-start acceleration to prevent sample splashing
• Infrared CO₂ sensor (NDIR type) with 0–20% measurement range, ±0.2% full-scale accuracy, and automatic zero-point compensation
• Integrated 0.3 µm HEPA-grade microbial filter on CO₂ inlet line, achieving ≥99.99% removal efficiency for airborne contaminants ≥0.3 µm
• Large-angle tempered glass observation window with internal LED illumination and UV sterilization option (254 nm, 15 W, timer-controlled)
• Electrostatically coated steel chassis and 304 stainless-steel interior chamber; fully autoclavable platform inserts and removable drip trays
• Intelligent safety suite: over-temperature cutoff, over-speed shutdown, door-open interruption, power-failure auto-restart with parameter retention

Sample Compatibility & Compliance

The ZYL.CP-5036R accommodates standard laboratory vessels—including Erlenmeyer flasks (up to 2000 mL), test tubes (13–25 mm diameter), microtiter plates (96-/384-well), and custom bioreactor bags—via interchangeable clamping systems (fixed flask holders, universal spring clips, tube racks, and irregular-bottle adapters). Its dual-door design minimizes thermal and CO₂ loss during access, while optional O₂-enrichment ports support aerobic high-density cultures. The unit complies with IEC 61010-1:2010 for laboratory equipment safety and meets electromagnetic compatibility requirements per EN 61326-1. For regulated environments, audit trail functionality (via optional USB data logger) supports 21 CFR Part 11 readiness when paired with validated software; temperature and CO₂ logs are timestamped, user-identified, and exportable in CSV format without modification.

Software & Data Management

A built-in microprocessor controller features a backlit 5.7-inch LCD interface with intuitive menu navigation and real-time graphical display of temperature, CO₂ concentration, RPM, and elapsed time. All operational parameters—including ramp profiles, multi-step timers, and alarm thresholds—are stored in non-volatile memory with password-protected editing. Optional USB interface enables direct download of continuous 1-second-interval datasets covering up to 30 days of operation. Exported files include metadata headers (instrument ID, operator, session start/end timestamps) and are compatible with MATLAB, Python pandas, and LIMS integration via serial command protocol (Modbus RTU). Firmware updates are performed offline using FAT32-formatted USB drives, ensuring continuity during GxP audits.

Applications

• Mammalian cell expansion in DMEM/F12 media with 5% CO₂ supplementation for monoclonal antibody production
• Suspension culture of insect cells (Sf9, Sf21) under low-shear orbital agitation for baculovirus expression systems
• Microaerophilic bacterial fermentation (e.g., Campylobacter jejuni) requiring precise O₂/CO₂ balance
• Enzymatic assays involving thermostable polymerases or dehydrogenases under controlled thermal cycling
• Stem cell differentiation protocols demanding synchronized mechanical stimulation and gas-phase regulation
• Environmental microbiology studies assessing pollutant degradation kinetics in mixed consortia under variable pCO₂

FAQ

Does the ZYL.CP-5036R support humidification?
No—this model is not equipped with active humidity control. For applications requiring >60% RH, consider the ZHL-RH18 series.
Can CO₂ concentration be calibrated in-house?
Yes—calibration is performed using certified reference gas (e.g., 5.00 ± 0.05% CO₂ in N₂) via the service menu; calibration coefficients are retained after power cycles.
Is the shaker compatible with sterile filtration manifolds?
Yes—the rear-mounted CO₂ and air inlet/outlet ports accept standard 1/4″ Swagelok fittings for integration into closed-loop perfusion systems.
What is the maximum load per platform?
Each platform supports up to 15 kg evenly distributed; total chamber capacity is 30 kg including vessel weight and medium volume.
How frequently should the CO₂ sensor be serviced?
NDIR sensor drift is typically <0.05% per year; annual verification against traceable gas standard is recommended for ISO 17025 compliance.