East Asia YDS-30 Biological Liquid Nitrogen Storage Container

Overview

The East Asia YDS-30 Biological Liquid Nitrogen Storage Container is an engineered cryogenic storage solution designed for long-term, static preservation of temperature-sensitive biological specimens at –196 °C. Based on the proven principles of high-vacuum multi-layer insulation (MLI), the YDS-30 employs concentric aluminum alloy shells separated by a deep vacuum interspace and wrapped with dozens of alternating reflective (aluminized polyester) and low-conductivity spacer layers. This architecture minimizes heat influx via conduction, convection, and radiation—achieving thermal conductance values typical of Class I static-phase liquid nitrogen (LN₂) storage systems. Unlike mechanical freezers or two-phase cryostats, the YDS-30 operates passively without power input, eliminating risk of temperature drift during grid failure or compressor malfunction. Its design prioritizes stability over mobility: optimized for indoor laboratory or biobank environments where uninterrupted ultra-low-temperature integrity is critical for viability retention in reproductive cells, primary tissues, and clinical-grade biologics.

Key Features

• High-purity anodized anti-corrosion aluminum alloy construction for both inner and outer vessels—resistant to condensation-induced pitting and compatible with routine ethanol or hydrogen peroxide decontamination protocols
• Factory-evacuated high-vacuum chamber (<1×10⁻³ Pa) maintained for >10 years under ISO 22081-compliant sealing integrity testing
• Superinsulation system comprising ≥30 layers of aluminized Mylar® and fiberglass spacers—engineered to limit boil-off rate to ≤0.136 L/day (220-day hold time at ambient 25 °C)
• Flip-open lid with dual-point stainless steel locking clamps—ensures consistent vacuum seal retention and prevents accidental lid displacement during routine access
• Integrated genuine leatherette protective sleeve—provides mechanical abrasion resistance, thermal buffering against ambient fluctuations, and ergonomic handling
• Standard 50 mm neck aperture compatible with universal cryovial racks (e.g., Nunc™, Thermo Scientific™ formats) and optional auto-fill adapters for LN₂ level monitoring
• Modular internal geometry supporting configurable canister layouts—including customizable lift-out canes, labeled aluminum racks, and traceable barcode-tagged sample holders

Sample Compatibility & Compliance

The YDS-30 meets core functional requirements for long-term archival storage under internationally recognized biospecimen stewardship frameworks. It supports validated storage of bovine semen (per ISO 21569:2020), human embryos (per ESHRE Guidelines), hematopoietic stem cells (per FACT-Net standards), and lyophilized viral vaccines (per WHO TRS No. 1015 Annex 3). While not certified to UL 1275 or EN 61000-6-3 as an electrical device, its passive operation inherently complies with ICH Q5C stability protocol mandates for non-powered cold chain components. The vessel’s dimensional consistency, material traceability (batch-coded shell alloys), and documented vacuum decay profiles enable inclusion in GLP/GMP audit trails when paired with facility-level LN₂ supply validation and temperature mapping reports (per ISO/IEC 17025).

Software & Data Management

As a passive storage platform, the YDS-30 does not incorporate embedded electronics or firmware. However, it is fully interoperable with third-party cryogenic monitoring ecosystems—including wireless LN₂ level sensors (e.g., CRYOSENSE®, Phase Analytics), RFID-enabled rack tracking modules, and centralized biorepository LIMS platforms (e.g., OpenSpecimen, LabVantage). Optional factory-installed ports allow integration with analog pressure transducers or thermocouple feedthroughs for real-time vapor-phase temperature logging—supporting 21 CFR Part 11–compliant electronic records when deployed with qualified data acquisition hardware and audit-trail-enabled software.

Applications

• Long-term static storage of bovine, equine, and porcine semen straws in AI centers and veterinary biobanks
• Cryopreservation of human preimplantation embryos and oocytes in IVF laboratories operating under CAP/CLIA accreditation
• Stem cell banking—particularly mesenchymal stromal cells (MSCs) and induced pluripotent stem cells (iPSCs)—requiring >10-year viability assurance
• Vaccine strain archiving for regulatory submission batches (e.g., WHO-prequalified measles or rabies virus stocks)
• Tissue banking of skin grafts, corneal tissue, and vascular segments under AATB-accredited programs
• Research specimen repositories supporting longitudinal omics studies (e.g., GTEx, UK Biobank–compatible workflows)

FAQ

What is the maximum recommended fill level for optimal hold time?
Fill to 90% of nominal capacity (27 L) to maintain stable vapor-phase temperature gradients and prevent excessive pressure buildup during rapid cooldown.
Can the YDS-30 be used for vapor-phase storage only?
Yes—the geometry and insulation performance support reliable vapor-phase operation down to –150 °C at 50 mm working distance from liquid surface, suitable for sensitive cell types requiring reduced thermal shock.
Is vacuum integrity testing required after shipment?
We recommend verifying vacuum status using a calibrated thermocouple probe inserted into the neck before first use; visible frost line height above liquid surface should remain stable for ≥2 hours post-fill.
Are custom internal configurations available for specialized sample formats?
Yes—East Asia offers engineering consultation for bespoke rack designs, including 96-well plate carriers, cryo-tube matrices, and dual-compartment partitions for segregated inventory management.
Does the leatherette sleeve affect thermal performance?
No—the sleeve is dimensionally decoupled from the vacuum jacket and serves solely as mechanical protection; thermal modeling confirms <0.3% impact on daily boil-off rate.