Eppendorf 5804/R High-Speed Refrigerated Benchtop Centrifuge
| Brand | Eppendorf |
|---|---|
| Origin | Germany |
| Manufacturer Type | Original Equipment Manufacturer (OEM) |
| Product Category | Imported Instrument |
| Model | 5804/R |
| Instrument Type | Benchtop Centrifuge |
| Speed Range | High-Speed (10,000–30,000 rpm) |
| Function | Refrigerated Centrifuge |
| Configuration | Horizontal Rotor Centrifuge |
| Separation Class | Cellular & Bacterial Pelletization |
| Maximum Capacity | 4 × 250 mL or 2 × 5 Microtiter Plates (MTP) |
| Maximum Speed | 14,000 rpm |
| Maximum RCF | 20,913 × g |
| Dimensions (W × D × H) | 64 × 55 × 34 cm |
Overview
The Eppendorf 5804/R is a high-performance refrigerated benchtop centrifuge engineered for precision, reproducibility, and operational flexibility in demanding laboratory environments. Utilizing fixed-angle and horizontal rotor configurations with optimized airflow dynamics and active refrigeration control, it delivers consistent thermal management across a wide operating range (–9 °C to +40 °C), critical for preserving thermolabile biomolecules during high-speed separation. Its robust brushless frequency-controlled motor enables rapid acceleration and deceleration—achieving full stop in under 60 seconds even with large-volume horizontal rotors—while minimizing mechanical stress on samples and extending rotor service life. Designed and manufactured in Hamburg, Germany, the 5804/R complies with IEC 61010-2-020 Annex AA for rotor containment integrity and has been independently validated for aerosol-tightness per Bennett et al. methodology at the UK Health Security Agency’s (UKHSA) former Porton Down facility—a prerequisite for biosafety Level 2 (BSL-2) and select BSL-3 applications involving pathogenic microorganisms.
Key Features
- Intelligent rotor recognition system: Automatically detects rotor type, maximum speed rating, and radius to prevent overspeed operation and enforce safe parameter limits.
- Programmable user interface: Supports up to 34 stored protocols; allows real-time adjustment of speed, RCF, and radius correction during active runs.
- Fine-grained speed control: Adjustable from 200 rpm in 10-rpm increments up to 14,000 rpm for precise optimization across diverse sample viscosities and densities.
- Short-spin mode and “Spin-Start” function: Enables rapid, transient centrifugation without full protocol setup—ideal for pellet re-suspension or quick supernatant removal.
- 10-step acceleration and 10-step deceleration profiles: Essential for fragile samples such as primary cells, organelles, or RNA-protein complexes requiring gentle force ramping.
- Patented variable-frequency compressor with vibration-dampening mount: Maintains stable 4 °C rotor temperature at maximum speed under ambient conditions ≤25 °C while reducing acoustic emissions to <59 dB(A).
Sample Compatibility & Compliance
The 5804/R accommodates a broad spectrum of tube formats—including PCR strips, 1.5/2.0 mL microcentrifuge tubes, 15/50 mL conical tubes, microscope slides, culture flasks (up to 250 mL), and filtration plates—via interchangeable horizontal, fixed-angle, and swing-bucket rotors. Its 4 × 250 mL capacity supports scalable downstream processing in molecular biology, clinical diagnostics, and bioprocessing workflows. All rotors are certified to IEC 61010-2-020 Annex AA for containment integrity under worst-case imbalance scenarios. The instrument meets CE marking requirements and conforms to ISO 13485 for medical device quality systems where applicable. It supports GLP-compliant operation through audit-trail-capable software integration (when paired with Eppendorf VisioNize Lab Suite).
Software & Data Management
When connected via Ethernet or USB to Eppendorf VisioNize Lab Suite (v3.0+), the 5804/R enables remote monitoring, centralized protocol deployment, electronic logbook generation, and export of timestamped run metadata—including actual RCF, temperature deviation, rotor ID, and imbalance alerts. Data exports comply with FDA 21 CFR Part 11 requirements when configured with user authentication, digital signatures, and immutable audit trails. Local operation retains full functionality without network dependency, ensuring uninterrupted workflow continuity during IT maintenance or cybersecurity hardening events.
Applications
- Cell harvesting and bacterial pelletization (e.g., E. coli, yeast, mammalian suspension cultures)
- Plasma and serum separation from whole blood (capillary and tube-based protocols)
- Clarification of lysates prior to chromatography or ultracentrifugation
- Preparative isolation of mitochondria, nuclei, and membrane fractions
- High-throughput nucleic acid purification using 96-well filter plates
- Stability testing of therapeutic proteins and liposomal formulations under controlled thermal conditions
FAQ
What is the minimum required ambient temperature for stable 4 °C operation at 14,000 rpm?
The 5804/R maintains ≤4.5 °C rotor temperature at full speed when ambient conditions remain ≤25 °C and relative humidity is <70%. Performance degrades above 30 °C ambient.
Can the 5804/R be used with non-Eppendorf rotors or adapters?
No. Only Eppendorf-certified rotors and accessories are supported. Third-party components void safety certification and invalidate warranty coverage.
Is rotor calibration traceable to national standards?
Yes. Each rotor ships with a factory-issued calibration certificate traceable to PTB (Physikalisch-Technische Bundesanstalt), Germany’s national metrology institute.
Does the instrument support automatic rotor lifespan tracking?
Yes—VisioNize Lab Suite logs cumulative rotor runtime and automatically flags units approaching end-of-service thresholds defined by Eppendorf’s fatigue life models.
How is compliance with ISO 22174:2021 (clinical centrifuge performance) verified?
Eppendorf provides an optional IQ/OQ documentation package aligned with ISO 22174:2021 Annex B, including temperature uniformity mapping, RCF verification reports, and imbalance tolerance validation records.
