Hanbon Bio-Lab30 Laboratory Chromatography System

Overview

The Hanbon Bio-Lab30 Laboratory Chromatography System is an engineered low-to-medium pressure liquid chromatography platform designed for robust, reproducible protein purification in academic research labs, biotech R&D facilities, and QC/QA environments. Built upon a modular architecture, the system implements standard chromatographic separation principles—including size-exclusion (SEC), ion-exchange (IEX), hydrophobic interaction (HIC), and affinity chromatography—using precisely controlled mobile phase gradients, real-time multi-parameter detection, and programmable fraction collection. Its core fluidic design adheres to LPLC/MPLC operational constraints (≤5 MPa), ensuring compatibility with common prepacked and self-packed columns (e.g., 10–26 mm ID, up to 20 cm bed height), while maintaining biomolecular integrity through minimized dwell volume, low-shear flow paths, and bio-inert wetted materials (e.g., PEEK, stainless steel 316L, and fluoropolymer seals).

Key Features

• High-precision dual-piston reciprocating pump with pulse-dampening and flow accuracy ±1% across 0.01–30 mL/min range, supporting gradient formation with ≤1% compositional error
• Multi-channel real-time detection: UV-Vis absorbance (200–400 nm, 1 nm resolution, ±0.002 AU baseline noise), differential pH measurement (±0.02 pH units), and conductivity monitoring (0.01–999.9 mS/cm, ±1% full scale)
• Stacked-tube fluidic layout reducing system dead volume to <120 µL (pump-to-detector), minimizing band broadening and improving resolution for dilute or labile samples
• Fully bio-compatible flow path: All sample-contact surfaces comply with USP Class VI and ISO 10993-5 cytotoxicity standards; no leachable metals or plasticizers detected under ICH Q5E stress conditions
• Modular valve manifold supporting up to 8 inlet solvents, 4 column positions, and automated column switching—enabling tandem, serial, or parallel purification workflows
• Integrated fraction collector with 96-well plate, rack, or tube-based options; trigger modes include time, UV threshold, peak apex, or conductivity inflection point

Sample Compatibility & Compliance

The Bio-Lab30 accommodates a broad spectrum of biological macromolecules—including monoclonal antibodies (mAbs), recombinant enzymes, fusion proteins, and viral vectors—without compromising structural stability or functional activity. Column compatibility spans resin chemistries from GE Cytiva, Tosoh, Bio-Rad, and custom-packed media (e.g., Ni-NTA, Protein A, Q Sepharose). The system meets essential regulatory prerequisites for non-GMP research use: full audit trail logging (user actions, method changes, run history), electronic signature support per FDA 21 CFR Part 11 (when deployed with validated software configuration), and traceability aligned with GLP documentation requirements. It supports method validation protocols per ICH Q2(R2) for parameters including precision, linearity, and robustness during process development.

Software & Data Management

Control and data acquisition are managed via Hanbon ChromoControl v3.x—a Windows-based application featuring intuitive drag-and-drop method editor, real-time overlay of UV/pH/conductivity traces, and automatic peak integration using second-derivative algorithms. Raw data are stored in vendor-neutral HDF5 format with embedded metadata (timestamp, operator ID, instrument configuration, calibration logs). Export options include CSV, PDF report generation (with customizable templates), and direct import into third-party analysis tools (e.g., GraphPad Prism, UNICORN 7.0 via XML export). Role-based user management supports ≥10 concurrent accounts with configurable permissions (operator, supervisor, administrator), and all method files are digitally signed to prevent unauthorized modification.

Applications

• Early-stage purification of His-tagged proteins using immobilized metal affinity chromatography (IMAC)
• Buffer exchange and aggregate removal via size-exclusion chromatography (SEC) under native conditions
• Polishing steps in mAb downstream processing—including cation-exchange (CEX) flow-through or bind-elute modes
• Process characterization studies requiring high-resolution fraction collection for mass spec or activity assay follow-up
• Method scouting and optimization for novel expression constructs or bispecific formats
• Training platform for chromatography fundamentals in university teaching laboratories

FAQ

Is the Bio-Lab30 compatible with prepacked columns from major suppliers such as Cytiva and Tosoh?
Yes—the system’s standard fittings (1/16″ Swagelok) and pressure rating (≤5 MPa) ensure seamless integration with most analytical and preparative-scale prepacked columns.
Can UV detection be calibrated traceably to NIST standards?
Yes—integrated holmium oxide and neutral density filters enable wavelength and photometric calibration per ASTM E275 and ISO 9001 internal calibration procedures.
Does the system support method transfer to AKTA platforms?
Yes—ChromoControl software exports gradient profiles, flow rates, and collection logic in XML format, facilitating rapid adaptation to GE Cytiva UNICORN workflows.
What maintenance intervals are recommended for pump seals and detector lamps?
Pump seals require replacement every 12 months or after 2,000 hours of operation; deuterium lamp lifetime is rated at ≥2,000 hours with auto-shutdown on intensity decay detection.
Is remote monitoring supported?
Yes—via optional secure VPN-enabled web interface (HTTPS/TLS 1.2), allowing real-time status viewing and alarm notification without direct network exposure.