Hanuo SPE-12 Manual Solid Phase Extraction System

Overview

The Hanuo SPE-12 Manual Solid Phase Extraction System is a precision-engineered benchtop manifold designed for reproducible, low-background sample preparation in analytical laboratories. It operates on the fundamental principle of solid-phase extraction—selective retention of analytes on a sorbent-packed cartridge via reversible physicochemical interactions (e.g., reversed-phase, ion exchange, or hydrophilic-lipophilic balance), followed by controlled elution under optimized solvent strength and pH conditions. Unlike automated platforms, the SPE-12 delivers full operator control over flow dynamics and timing, making it particularly suitable for method development, validation studies, and laboratories requiring flexibility across diverse matrix types (e.g., aqueous environmental extracts, biological fluids, food homogenates). Its fully borosilicate glass construction ensures chemical inertness toward aggressive solvents—including acetonitrile, methanol, dichloromethane, and dilute acids/bases—eliminating leachable contaminants that compromise LC-MS or GC-MS trace analysis.

Key Features

• Twelve independently controllable extraction channels, each equipped with a high-precision进口 (imported) PTFE-tipped flow regulation valve for fine-grained manual adjustment across the 0.1–30 mL/min range.
• Modular spiral support rack with height-adjustable geometry and swappable orifice plates (square and round configurations supported), enabling compatibility with standard 13 mm, 16 mm, and 25 mm diameter SPE cartridges as well as wide-bore collection tubes (12 × 15 mL or 12 × 50 mL formats).
• Vacuum chamber fabricated from thick-walled, annealed borosilicate glass with seamless one-piece molding—providing superior resistance to thermal shock, mechanical stress, and prolonged exposure to halogenated and polar organic solvents.
• Dual-seal vacuum槽 design incorporating anti-misting baffles and channel-isolated gas pathways to prevent cross-contamination between adjacent cartridges during simultaneous processing.
• Integrated analog vacuum gauge calibrated to –0.1 to 0 MPa (absolute), enabling real-time monitoring and stabilization of vacuum pressure at optimal levels (typically –0.06 to –0.08 MPa) for consistent breakthrough-free loading and washing steps.
• Chemically resistant high-molecular-weight polymer cartridge tray—dimensionally stable under sustained vacuum loads (>72 h continuous operation) and resistant to deformation or creep even at elevated ambient temperatures (up to 40 °C).

Sample Compatibility & Compliance

The SPE-12 supports all commercially available 1–6 mL format SPE cartridges, including silica-based C18, C8, SCX, SAX, Florisil®, and mixed-mode sorbents. Its glass-only fluid path meets stringent requirements for trace-metal analysis (EPA Method 1694, ISO 21675:2019) and avoids metal ion leaching associated with polymeric manifolds. The system is compatible with GLP-compliant workflows when used in conjunction with documented SOPs and calibrated vacuum sources. While not inherently 21 CFR Part 11 compliant (as a manual, non-data-logging device), it integrates seamlessly into validated sample prep sequences aligned with USP , ASTM D7237, and EN ISO 17892-9 protocols.

Software & Data Management

As a manually operated platform, the SPE-12 does not incorporate embedded firmware or digital interfaces. All operational parameters—including vacuum setpoint, flow rate per channel, solvent sequence, and dwell times—are recorded externally in laboratory notebooks or LIMS-integrated electronic lab notebooks (ELNs). This architecture aligns with audit-trail requirements for regulated environments where procedural transparency and operator accountability are prioritized over automation-driven data capture.

Applications

The system is routinely deployed in environmental testing labs for the cleanup of pesticide residues (e.g., organophosphates, neonicotinoids) from surface water per EPA Method 525.3; in clinical toxicology for opioid and benzodiazepine isolation from plasma prior to UHPLC-MS/MS quantification; in food safety laboratories for mycotoxin enrichment (e.g., aflatoxins B1/B2/G1/G2) from cereal extracts; and in pharmaceutical QC for residual solvent removal from active pharmaceutical ingredient (API) filtrates. Its robustness and material compatibility also support method transfer from R&D to routine QA/QC settings without revalidation.

FAQ

Is the SPE-12 compatible with positive-pressure nitrogen manifold conversion kits?
Yes—its standardized 1/4″ NPT vacuum inlet port allows direct integration with external nitrogen pressure regulators and gas distribution manifolds for pressure-assisted SPE, provided appropriate back-pressure regulation is implemented.
Can I use it with 6 mL cartridges containing >1 g sorbent mass?
Yes—the adjustable support rack accommodates cartridges up to 100 mm in height and 25 mm in diameter, supporting high-capacity formats used in large-volume environmental sampling (e.g., 1 L water extracts).
Does the glass vacuum chamber require periodic calibration?
No—the chamber itself is a passive structural component; only the analog vacuum gauge requires annual verification against a NIST-traceable reference standard.
What maintenance is required for long-term reliability?
Routine cleaning with methanol/water (1:1) followed by air-drying; inspection of valve O-rings every 6 months; replacement of PTFE valve tips only if visible wear or inconsistent flow regulation occurs.
Is technical documentation available in English with CE or RoHS declarations?
Yes—full English-language user manual, material compliance certificates (RoHS 2011/65/EU, REACH SVHC), and dimensional drawings are supplied upon request for import clearance and internal quality audits.