High-Fidelity Skin-on-a-Chip Testing System
| Brand | RELEGO |
|---|---|
| Model | RELEGO |
| Origin | Singapore |
| Import Status | Imported |
| Distribution Type | Authorized Distributor |
| Pricing | Upon Request |
Overview
The RELEGO High-Fidelity Skin-on-a-Chip Testing System is an integrated microphysiological platform engineered for next-generation in vitro human skin modeling. It combines 3D bioprinted or scaffold-based full-thickness human skin tissue constructs with a precisely controlled microfluidic perfusion circuit to emulate key physiological parameters—including interstitial flow, barrier integrity, metabolic activity, and immune cell trafficking—under dynamic culture conditions. Unlike static transwell-based skin equivalents, this system applies physiologically relevant shear stress (0.1–5 dyn/cm²), enables continuous nutrient/waste exchange via endothelialized microchannels, and supports long-term maintenance (>21 days) of epidermal stratification, dermal fibroblast quiescence, and functional tight junction formation. The platform operates on established principles of organ-on-a-chip biomechanics and is validated for quantitative assessment of percutaneous absorption kinetics, cytotoxicity endpoints (e.g., IL-1α, LDH, MTT), and active ingredient penetration profiling using confocal microscopy and mass spectrometry–compatible sampling.
Key Features
- Modular dual-chamber architecture integrating epidermal and dermal compartments with optically transparent PDMS microfluidic channels for real-time imaging and TEER monitoring
- Integrated peristaltic pump system delivering tunable flow rates (0.5–50 µL/min) with pressure feedback control to maintain physiological perfusion pressure (5–25 mmHg)
- Compatible with primary human keratinocytes, melanocytes, fibroblasts, and co-cultured endothelial cells (HUVEC/HMVEC) on collagen IV/fibronectin-coated membranes
- Standardized 24-well plate footprint enabling high-throughput screening (HTS) integration with automated liquid handlers and plate readers
- Temperature- and CO₂-controlled incubation interface supporting long-term culture at 37°C and 5% CO₂ without medium evaporation or channel clogging
- Pre-validated assay protocols aligned with OECD TG 439 (skin irritation), TG 431 (skin corrosion), and ISO 10993-10 (irritation/sensitization)
Sample Compatibility & Compliance
The system accommodates both commercially available and user-differentiated 3D skin equivalents—including reconstructed epidermis (EpiDerm™, SkinEthic™), full-thickness models (Phenion® FT, EpiSkin™), and custom vascularized constructs incorporating CD31⁺ endothelial networks. All chip substrates are sterilized via gamma irradiation and certified endotoxin-free (<0.03 EU/mL). Device compliance includes adherence to ISO 13485:2016 for medical device quality management systems, CE marking under Directive 2014/31/EU for in vitro diagnostic devices, and compatibility with GLP-compliant study execution per OECD Principles of Good Laboratory Practice. Data traceability meets FDA 21 CFR Part 11 requirements when used with validated LIMS or ELN software integrations.
Software & Data Management
The RELEGO Control Suite (v3.2+) provides closed-loop hardware orchestration, including real-time flow rate calibration, temperature stabilization logs, and pressure transient analysis. All experimental metadata—including passage number, donor ID, matrix batch, and perfusion history—is automatically embedded into exported CSV and HDF5 files. Time-synchronized image acquisition supports FIJI/ImageJ macro automation and integrates natively with commercial analysis platforms (e.g., Imaris, HALO) for 3D reconstruction and fluorescence quantification. Audit trails record operator actions, parameter modifications, and firmware updates with digital signatures, satisfying regulatory audit requirements for preclinical dossier submissions.
Applications
- Quantitative evaluation of topical drug permeation kinetics (e.g., corticosteroids, retinoids, peptides) using Franz diffusion cell–compatible sampling ports
- Toxicity screening of cosmetic actives and preservatives under dynamic exposure conditions mimicking daily use regimens
- Mechanistic studies of nanoparticle transdermal delivery and follicular targeting via multiphoton microscopy coupling
- Immunomodulatory profiling of contact allergens through dendritic cell migration assays and cytokine multiplexing (IL-18, TNF-α, IFN-γ)
- Regulatory submission support for REACH, Cosmetics Regulation (EC) No 1223/2009, and FDA IND-enabling nonclinical data packages
FAQ
Is the RELEGO Skin-on-a-Chip system compatible with existing 3D skin tissue suppliers?
Yes—standardized membrane dimensions (12 mm diameter, 0.4 µm pore size) ensure interoperability with major commercial tissue providers and academic biobanks.
Can the system be operated under sterile conditions for extended co-culture experiments?
Absolutely—the chip housing is autoclavable, and all fluidic paths support aseptic connection via Luer-lock fittings and 0.22 µm in-line filters.
Does RELEGO provide validation reports for OECD guideline alignment?
Yes—comprehensive technical dossiers including protocol verification, inter-laboratory reproducibility data (n=5 sites), and benchmarking against rabbit skin and human volunteer studies are available under NDA.
What level of technical support is offered post-purchase?
RELEGO provides remote troubleshooting, on-site installation and training (optional), annual performance verification, and access to a dedicated application scientist team for assay development collaboration.
