HUANKONG JHK-3000 Air Microbial Sampler

Overview

The HUANKONG JHK-3000 Air Microbial Sampler is a precision-engineered, portable impaction-based instrument designed for quantitative collection and enumeration of viable airborne microorganisms—including bacteria, fungi, and viral particles—in controlled and uncontrolled environments. It operates on the principle of inertial impaction: air is drawn at a calibrated flow rate through precisely engineered sieve-like nozzles; microbial-laden particles with sufficient aerodynamic mass accelerate and impact onto the surface of nutrient agar in Petri dishes. Following incubation, colony-forming units (CFUs) are enumerated, enabling calculation of viable microbial concentration per unit volume of air (e.g., CFU/m³). Unlike passive or filtration-based methods, impaction preserves biological viability while minimizing desiccation stress—critical for downstream culturing, strain isolation, and phenotypic characterization. The JHK-3000 meets the metrological and procedural requirements defined in JJF 1826–2020 (Calibration Specification for Air Microbial Samplers) and supports compliance with national ambient and indoor air quality standards including GB/T 18883–2022 and GB/T 18204.3–2013.

Key Features

• Portable, lightweight chassis with ergonomic carry handle—optimized for field deployment, cleanroom audits, and mobile environmental monitoring.
• Brushless high-vacuum sampling pump paired with digital mass flow control ensures stable, low-drift flow performance across the full 5–30 L/min range, meeting ISO 8573-1 purity class requirements for compressed air systems used in pharmaceutical manufacturing.
• Integrated lithium-ion battery provides ≥8 hours of uninterrupted operation under typical sampling conditions (20 L/min, 25 °C), eliminating dependency on external power sources during facility walkthroughs or emergency response.
• Onboard environmental sensors continuously monitor ambient temperature and atmospheric pressure, enabling real-time correction of sampled volume to standard conditions (25 °C, 101.325 kPa) per GB/T 18204.3–2013 Annex A.
• Large-capacity non-volatile memory stores ≥8,000 complete sampling records—including date/time stamp, flow setpoint, actual flow, duration, temperature, pressure, and calculated standard-volume—supporting traceability and GLP-aligned data integrity.
• Programmable sampling modes: single-run, timed start/stop, and multi-cycle interval sampling with user-defined intervals and repetitions—ideal for time-series exposure assessment in HVAC validation or hospital ward surveillance.
• Robust firmware architecture includes automatic power-failure recovery: upon restoration of supply, the device resumes sampling from the exact point of interruption, preserving chronological continuity and audit readiness.

Sample Compatibility & Compliance

The JHK-3000 supports multiple sampling modalities via interchangeable head configurations. Standard operation employs a single-stage sieve-plate impactor compatible with 90 mm Petri dishes. Optional accessories include Andersen-type multi-stage impactors (2-, 6-, or 8-stage) for size-resolved aerosol fractionation—enabling particle-size distribution analysis aligned with ISO 14644-1 particle counting protocols. A liquid impinger adapter is available for collecting fragile or hydrophilic bioaerosols (e.g., enveloped viruses, thermolabile spores) into sterile collection fluid for PCR or qPCR analysis. All hardware and firmware comply with Chinese national standards for public health microbiology sampling, including GB/T 18204.5–2013 (HVAC system inspection) and JJF 1826–2020 calibration traceability requirements. While not CE-marked or FDA 510(k)-cleared, its operational parameters and documentation structure align with foundational elements of ISO/IEC 17025 and EU GMP Annex 1 (2022) recommendations for environmental monitoring in aseptic processing.

Software & Data Management

Data retrieval is performed via standard USB-A interface using commercially available USB flash drives (FAT32 formatted). Exported files are comma-separated values (CSV) with UTF-8 encoding, containing all recorded metadata and calculated parameters. No proprietary software installation is required—data are directly importable into Excel, MATLAB, or LIMS platforms. Each record includes unique session ID, operator code (user-definable), and checksum-verified timestamps compliant with ALCOA+ principles (Attributable, Legible, Contemporaneous, Original, Accurate, Complete, Consistent, Enduring, Available). Audit trail functionality is embedded at firmware level: all parameter changes, mode transitions, and power events are logged with millisecond-resolution timestamps—facilitating regulatory review under GLP or GMP frameworks.

Applications

• Hospital infection control: routine monitoring of operating theaters, isolation rooms, and immunocompromised patient wards per GB/T 18204.3–2013 thresholds.
• Pharmaceutical manufacturing: environmental monitoring of Grade A–D cleanrooms during media fill simulations and routine qualification per EU GMP Annex 1 and USP .
• Food processing facilities: verification of air sanitation efficacy before and after fogging or UVGI interventions.
• Public health laboratories: outbreak investigation support, including pathogen source tracking in schools, nursing homes, and transportation hubs.
• Academic research: aerosol generation studies, filter efficiency testing, and bioaerosol transport modeling under controlled climatic conditions.
• HVAC system validation: airflow pattern assessment and microbial load mapping across ductwork, AHUs, and terminal HEPA filters per GB/T 18204.5–2013.

FAQ

What sampling standards does the JHK-3000 support?
It complies with JJF 1826–2020 (calibration), GB/T 18204.3–2013 (general microbiological sampling), GB/T 18883–2022 (indoor air quality), and GB/T 18204.5–2013 (HVAC systems).
Can the instrument be used for virus sampling?
Yes—when configured with the optional liquid impinger adapter and appropriate collection media (e.g., viral transport medium), it enables recovery of culturable and molecularly detectable viral aerosols.
Does the device correct for temperature and pressure automatically?
Yes—integrated sensors measure ambient temperature and barometric pressure in real time, and the firmware applies ISO 10780-compliant algorithms to calculate standard-condition sampling volume.
Is remote data transmission supported?
No—the JHK-3000 does not feature Wi-Fi, Bluetooth, or cellular connectivity. Data export is USB-only, prioritizing security and regulatory simplicity.
What is the recommended maintenance interval?
Calibration verification is advised every 6 months or after 200 operating hours, per JJF 1826–2020; pump filter replacement is recommended quarterly under continuous use.